ID A0QSH0_MYCS2 Unreviewed; 594 AA.
AC A0QSH0;
DT 09-JAN-2007, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2007, sequence version 1.
DT 27-MAR-2024, entry version 117.
DE SubName: Full=Signal peptide peptidase SppA, 67K type {ECO:0000313|EMBL:ABK69813.1};
DE EC=3.4.-.- {ECO:0000313|EMBL:ABK69813.1};
GN Name=sppA {ECO:0000313|EMBL:ABK69813.1};
GN OrderedLocusNames=MSMEG_1476 {ECO:0000313|EMBL:ABK69813.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196 {ECO:0000313|EMBL:ABK69813.1, ECO:0000313|Proteomes:UP000000757};
RN [1] {ECO:0000313|EMBL:ABK69813.1, ECO:0000313|Proteomes:UP000000757}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155 {ECO:0000313|Proteomes:UP000000757};
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S49 family.
CC {ECO:0000256|ARBA:ARBA00008683}.
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DR EMBL; CP000480; ABK69813.1; -; Genomic_DNA.
DR RefSeq; WP_011727691.1; NZ_SIJM01000016.1.
DR RefSeq; YP_885858.1; NC_008596.1.
DR AlphaFoldDB; A0QSH0; -.
DR STRING; 246196.MSMEG_1476; -.
DR PaxDb; 246196-MSMEI_1440; -.
DR GeneID; 66732933; -.
DR KEGG; msm:MSMEG_1476; -.
DR PATRIC; fig|246196.19.peg.1461; -.
DR eggNOG; COG0616; Bacteria.
DR OrthoDB; 9764363at2; -.
DR Proteomes; UP000000757; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR CDD; cd07018; S49_SppA_67K_type; 1.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR Gene3D; 6.20.330.10; -; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR004634; Pept_S49_pIV.
DR InterPro; IPR004635; Pept_S49_SppA.
DR InterPro; IPR002142; Peptidase_S49.
DR InterPro; IPR047217; S49_SppA_67K_type_N.
DR InterPro; IPR047272; S49_SppA_C.
DR NCBIfam; TIGR00705; SppA_67K; 1.
DR NCBIfam; TIGR00706; SppA_dom; 1.
DR PANTHER; PTHR33209; PROTEASE 4; 1.
DR PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR Pfam; PF01343; Peptidase_S49; 2.
DR PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABK69813.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000000757};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825}.
FT DOMAIN 109..257
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT DOMAIN 374..524
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT ACT_SITE 175
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT ACT_SITE 391
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ SEQUENCE 594 AA; 62715 MW; 624770D69078AA3F CRC64;
MFGFLPGIPG QDDLRALVRR VDTARHNGVP DGCVLELDLM SIPPETTGFD PLAMIAGGGR
PLVLRQAVAA IHRAAEDDRV AGLIARVQLP AAAPGPVQEL RDAIVAFGER KPTVAWAETY
PGTMSYYLAS AFREIWMQPA GSVGLIGFAT NATFLRDALD KAGIEAQFIA RGEYKSAANV
FTEGSYTEPH READARLIES LQEQVWQGIA QSRGLDPDTI NTLADQAPLL REAAVTAGLV
DRIGFRDEAY KRIGELAGGP ENTDADHDDA PPRLFLSRYA KATAPKPAPP LPSLPGRKSK
PTIAVVTLHG AIVSGRGGPQ PLPFGRSSAG GDTIAAALRE AAANDDVAAV VLRVDSPGGS
VTGSETVWRE VVRTRDAGKP VVASMGAVAA SGGYYVSMAA DEIVANAGTI TGSIGVLTGK
FVSRELKDKL GVGSDAVRTN ANADAWSTNA PFTDEQHARV EAEADLLYRD FVERAAEGRK
LPIEEMESLA RGRVWTGSDA KERGLVDHVG GLRAAVTRAK VLAGFDEDAD ARVVAYPGSS
WLDMLRPKPS SQPAAASLPE ALAALLGKSV IGVLDQAERS MTGASALWLG EYRF
//
