ID   A0YG12_9GAMM            Unreviewed;       941 AA.
AC   A0YG12;
DT   23-JAN-2007, integrated into UniProtKB/TrEMBL.
DT   23-JAN-2007, sequence version 1.
DT   27-MAR-2024, entry version 60.
DE   SubName: Full=Zinc protease {ECO:0000313|EMBL:EAW30262.1};
GN   ORFNames=GP2143_01925 {ECO:0000313|EMBL:EAW30262.1};
OS   marine gamma proteobacterium HTCC2143.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
OC   Spongiibacteraceae; BD1-7 clade.
OX   NCBI_TaxID=247633 {ECO:0000313|EMBL:EAW30262.1, ECO:0000313|Proteomes:UP000004931};
RN   [1] {ECO:0000313|EMBL:EAW30262.1, ECO:0000313|Proteomes:UP000004931}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTCC2143 {ECO:0000313|EMBL:EAW30262.1,
RC   ECO:0000313|Proteomes:UP000004931};
RX   PubMed=20601481; DOI=10.1128/JB.00683-10;
RA   Oh H.M., Kang I., Ferriera S., Giovannoni S.J., Cho J.C.;
RT   "Genome sequence of the oligotrophic marine Gammaproteobacterium HTCC2143,
RT   isolated from the Oregon Coast.";
RL   J. Bacteriol. 192:4530-4531(2010).
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EAW30262.1}.
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DR   EMBL; AAVT01000009; EAW30262.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0YG12; -.
DR   STRING; 247633.GP2143_01925; -.
DR   eggNOG; COG0612; Bacteria.
DR   Proteomes; UP000004931; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:EAW30262.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000004931};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          81..224
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          233..408
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          688..868
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   941 AA;  105230 MW;  3ED675CA5263DA9B CRC64;
     MANAPQSIGR RNIINVLESE LPKGEFVFSL YRVKRLFVIS TLTAVLTPVA ITAQAAAKLQ
     PITSVEGITE YRLDNGLQVL LFPDQTKETV TVNVTYHVGS KHENYGETGM AHLLEHLVFK
     GTPRHKDIPS ELSSHGARPN GSTWTDRTNY FETFSATEEN IEWALDMEAD RMVNSFIAKK
     DLDSEMTVVR NELERGENSP FRVTLQRIMS SAYTWHNYGK STIGARSDLE NVPIDRLQAF
     YRKYYQPDNA TLIVAGKFDN ADMLQRVSKY FGGIPKPVRT LTRTYTEEPA QDGEKMITVR
     RVGDVQLFMS AYHIPAGSHP DYAALDVLSQ VLGDTPSGRL HKQLVEKNLA SRAFASNFQW
     RDPGVAIFGI QIDKEGDLAA SSEHMLSVLE NISTLGITDA EVERVKRNIL KNIELSFNSS
     ERFALNLSEW LGMGDWRLFF MHRDRVEKVT TTDVQRVAEA YLQANNRTAG RFIPTEKPAR
     IAIPMVANID GMLDGYVGKP AIAQGENFEP SFDNIDKRTE ILQLENGVDV ALLAKKTRGE
     TVVVSIQLGL GDEQSLIGMR ATGSAVGAML MRGTNDLSRE QLQIEFDKLK ANVYVGASAS
     SAYAKVTTTR ENLSATLRLV ATIFKNPAFN KDEFSQYKST LEVDIEQNLQ DPQQLAFREY
     ARKQNPFPPE HPLYQPTLEQ ELAAVKALKH EDLGSFHRRF YGASHMQIGL IGDFEKSTVL
     SELEMIFGKW TSTTDYQRIP HPFQRVSTKT SNFNTADKEN AVFLATMAIP IGDDSDDAAA
     LELGNYIFGG GFLNSRLATR LRQQDGLSYS VRSILSQSPF DNRTSIAAFA ICAPQNLSRV
     EQGFKEELLN MLDNGFTEAE IVAAKSGLLQ SKKVSRAQDN ELVEVQVNLL ELGRSMQWSK
     AYEDRLEALS AEDVKTVMNK YLKVEDFSLI KAGDMSKIET P
//