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Database: UniProt
Entry: A1D1E3
LinkDB: A1D1E3
Original site: A1D1E3 
ID   ROK1_NEOFI              Reviewed;         738 AA.
AC   A1D1E3;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   16-OCT-2019, entry version 69.
DE   RecName: Full=ATP-dependent RNA helicase rok1;
DE            EC=3.6.4.13;
GN   Name=rok1; ORFNames=NFIA_009150;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC
OS   A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 /
RC   NRRL 181 / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P.,
RA   Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A.,
RA   Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A.,
RA   Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R.,
RA   Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M.,
RA   Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R.,
RA   Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R.,
RA   Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal
CC       subunit biogenesis. Required for the processing and cleavage of
CC       35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 snoRNA and is associated with the
CC       90S and 40S pre-ribosomes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1
CC       subfamily. {ECO:0000305}.
DR   EMBL; DS027688; EAW22236.1; -; Genomic_DNA.
DR   RefSeq; XP_001264133.1; XM_001264132.1.
DR   SMR; A1D1E3; -.
DR   STRING; 36630.CADNFIAP00001418; -.
DR   PRIDE; A1D1E3; -.
DR   EnsemblFungi; EAW22236; EAW22236; NFIA_009150.
DR   GeneID; 4591949; -.
DR   KEGG; nfi:NFIA_009150; -.
DR   EuPathDB; FungiDB:NFIA_009150; -.
DR   HOGENOM; HOG000242486; -.
DR   KO; K14779; -.
DR   OMA; HKIKVTD; -.
DR   OrthoDB; 400908at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    738       ATP-dependent RNA helicase rok1.
FT                                /FTId=PRO_0000282702.
FT   DOMAIN      236    448       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      488    656       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     249    256       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       191    219       Q motif.
FT   MOTIF       395    398       DEAD box.
SQ   SEQUENCE   738 AA;  82229 MW;  3C91F9397D9A6872 CRC64;
     MDAFKLLTRA TKLKGGAAPS SAQSSTRLPS TGKAANPQLF RSSEADKVLE EARHGKKRKR
     VAGPEAAESD DDGAELNFFG SSKARRSSAS MAKEQEKEQQ EQQEHRDDTS DADDADEMDE
     VQCRTVLNAH KIKVTDMRDL EEIQTVRVES EEPKKKKKKR KQQEESTPAL TKKEQKKARR
     VYPQPLVSFK ELRTRYKISR RLAENIAEQG FSVPTEVQLG TLPLLLEGFK VSGNSKDAES
     IEPDLLVVAP TGSGKTLSFL IPVINKIVRH HHEQEEERGI FSVVVAPTKE LASQIVNEGR
     KLVHGTGVKI TLMKKGMRVV DREDDDDENS HSEDSEEGSE SEQDEPSTTR KKKGKAPVTK
     SDILVTTPLL LVNALSANRT KPMATLPLVR NIVLDEADVL LDELFRDQTL DIWRACTHPE
     LRASLWSATM GSNIEDLAKS TIKERKQAYD RTNSYPLLRL VVGLKDSAIP NIEHKLVYAA
     TEQGKLLGLR QLLHPAAASA SDVRLRPPFL IFTQTIPRAI ALHSELRYDI PAEAGGSSRI
     AVLHSDLSDG QRSEIMKNFR KGEIWILVTT DLLARGVDFR GINGVVNYDI PNSAAVYVHR
     VGRTGRAGRE GGIAVTYYTK EDIPYVKSIA NIIDVSEKLR GKDGEKSIQK WLLDALPDLS
     KKDKKELKKH GVKARQSNLK SVKDDKEHRK TRISTKSGFD RRMENKKKAL IAASRNRKSK
     TQSTDPGSDN ESWDGLDG
//
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