ID A1DAM8_NEOFI Unreviewed; 318 AA.
AC A1DAM8;
DT 23-JAN-2007, integrated into UniProtKB/TrEMBL.
DT 23-JAN-2007, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=nitrilase {ECO:0000256|ARBA:ARBA00039045};
DE EC=3.5.5.1 {ECO:0000256|ARBA:ARBA00039045};
GN ORFNames=NFIA_095380 {ECO:0000313|EMBL:EAW19918.1};
OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=331117 {ECO:0000313|EMBL:EAW19918.1, ECO:0000313|Proteomes:UP000006702};
RN [1] {ECO:0000313|Proteomes:UP000006702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 /
RC NRRL 181 / WB 181 {ECO:0000313|Proteomes:UP000006702};
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00036406};
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS027694; EAW19918.1; -; Genomic_DNA.
DR RefSeq; XP_001261815.1; XM_001261814.1.
DR AlphaFoldDB; A1DAM8; -.
DR STRING; 331117.A1DAM8; -.
DR EnsemblFungi; EAW19918; EAW19918; NFIA_095380.
DR GeneID; 4588848; -.
DR KEGG; nfi:NFIA_095380; -.
DR VEuPathDB; FungiDB:NFIA_095380; -.
DR eggNOG; KOG0805; Eukaryota.
DR HOGENOM; CLU_030130_6_0_1; -.
DR OMA; AWLYHET; -.
DR OrthoDB; 2785533at2759; -.
DR Proteomes; UP000006702; Unassembled WGS sequence.
DR GO; GO:0000257; F:nitrilase activity; IEA:UniProt.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd07564; nitrilases_CHs; 1.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR InterPro; IPR044149; Nitrilases_CHs.
DR PANTHER; PTHR46044:SF1; CN HYDROLASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR46044; NITRILASE; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
DR PROSITE; PS00921; NITRIL_CHT_2; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000006702}.
FT DOMAIN 4..280
FT /note="CN hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS50263"
SQ SEQUENCE 318 AA; 34983 MW; 0128753F0EEBD4A5 CRC64;
MTKVRVGAVQ AEPAWNDLQG GVAKTIKLIK EAGEKGINVL GFPEVWIPGY LWSMWTNSPI
DNVQLFHEYM ANSLARNSPE MDAIRAAVKE AGIFIVLGYS ERDAGSIYMA QSFISPEGEI
VHHRRKLKPT HVERCIWGES QADSLKTVVD SPFGKIGALN CWEHLQPLLR YYEYEQGAQI
HVASWPAMFP MVKSVPWGFS ATGAGCQLMS QFMAIEGQTF VLACTQILTK ENLAKQNLVE
EGIIQVPGGG FAMIFGPDGT PLVEAPPPGV ECILQADIDL QHIDYAKALA DPVGHYSRPD
LLQLKVNKTA AKCVVDME
//
