ID A1K595_AZOSB Unreviewed; 158 AA.
AC A1K595;
DT 06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2007, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=Transcription elongation factor GreA {ECO:0000256|ARBA:ARBA00013729, ECO:0000256|HAMAP-Rule:MF_00105};
DE AltName: Full=Transcript cleavage factor GreA {ECO:0000256|HAMAP-Rule:MF_00105};
GN Name=greA {ECO:0000256|HAMAP-Rule:MF_00105,
GN ECO:0000313|EMBL:CAL94000.1};
GN OrderedLocusNames=azo1383 {ECO:0000313|EMBL:CAL94000.1};
OS Azoarcus sp. (strain BH72).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Azoarcus.
OX NCBI_TaxID=418699 {ECO:0000313|EMBL:CAL94000.1, ECO:0000313|Proteomes:UP000002588};
RN [1] {ECO:0000313|EMBL:CAL94000.1, ECO:0000313|Proteomes:UP000002588}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH72 {ECO:0000313|EMBL:CAL94000.1,
RC ECO:0000313|Proteomes:UP000002588};
RX PubMed=17057704; DOI=10.1038/nbt1243;
RA Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT strain BH72.";
RL Nat. Biotechnol. 24:1385-1391(2006).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC {ECO:0000256|ARBA:ARBA00024916, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family.
CC {ECO:0000256|ARBA:ARBA00008213, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
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DR EMBL; AM406670; CAL94000.1; -; Genomic_DNA.
DR RefSeq; WP_011765116.1; NZ_CP016210.1.
DR AlphaFoldDB; A1K595; -.
DR STRING; 62928.azo1383; -.
DR KEGG; aoa:dqs_1506; -.
DR KEGG; azo:azo1383; -.
DR eggNOG; COG0782; Bacteria.
DR HOGENOM; CLU_101379_2_0_4; -.
DR OrthoDB; 9808774at2; -.
DR Proteomes; UP000002588; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR NCBIfam; TIGR01462; greA; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF4; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00829; GREAB_1; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00105}; Elongation factor {ECO:0000313|EMBL:CAL94000.1};
KW Protein biosynthesis {ECO:0000313|EMBL:CAL94000.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002588};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00105};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00105}.
FT DOMAIN 5..74
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 84..157
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
SQ SEQUENCE 158 AA; 17212 MW; 477ABA7E13D598E3 CRC64;
MNKTPLTIAG AEKLRLELHR LKTVDRPNVI AAIAEARSHG DLSENAEYDA AKERQGFIEG
RIAEVEGKLA NAQIIDPTLL DADGRCVFGA TVELEDMDSG QVVTYQIVGE DEADIKEFKI
SVSSPIARAL IGKYAGDIAE VQAPGGIREY EIIDVRYI
//
