ID A1SKL4_NOCSJ Unreviewed; 426 AA.
AC A1SKL4;
DT 06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2007, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE SubName: Full=3-ketoacyl-CoA thiolase {ECO:0000313|EMBL:ABL82349.1};
DE EC=2.3.1.16 {ECO:0000313|EMBL:ABL82349.1};
GN OrderedLocusNames=Noca_2847 {ECO:0000313|EMBL:ABL82349.1};
OS Nocardioides sp. (strain ATCC BAA-499 / JS614).
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=196162 {ECO:0000313|EMBL:ABL82349.1, ECO:0000313|Proteomes:UP000000640};
RN [1] {ECO:0000313|Proteomes:UP000000640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-499 / JS614 {ECO:0000313|Proteomes:UP000000640};
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J.,
RA Richardson P.;
RT "Complete sequence of chromosome 1 of Nocardioides sp. JS614.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ABL82349.1, ECO:0000313|Proteomes:UP000000640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-499 / JS614 {ECO:0000313|Proteomes:UP000000640};
RX PubMed=21551312; DOI=10.1128/JB.05109-11;
RA Coleman N.V., Wilson N.L., Barry K., Brettin T.S., Bruce D.C., Copeland A.,
RA Dalin E., Detter J.C., Del Rio T.G., Goodwin L.A., Hammon N.M., Han S.,
RA Hauser L.J., Israni S., Kim E., Kyrpides N., Land M.L., Lapidus A.,
RA Larimer F.W., Lucas S., Pitluck S., Richardson P., Schmutz J., Tapia R.,
RA Thompson S., Tice H.N., Spain J.C., Gossett J.G., Mattes T.E.;
RT "Genome Sequence of the ethene- and vinyl chloride-oxidizing actinomycete
RT Nocardioides sp. strain JS614.";
RL J. Bacteriol. 193:3399-3400(2011).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|RuleBase:RU003557}.
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DR EMBL; CP000509; ABL82349.1; -; Genomic_DNA.
DR RefSeq; WP_011756287.1; NC_008699.1.
DR AlphaFoldDB; A1SKL4; -.
DR STRING; 196162.Noca_2847; -.
DR KEGG; nca:Noca_2847; -.
DR eggNOG; COG0183; Bacteria.
DR HOGENOM; CLU_031026_2_0_11; -.
DR OrthoDB; 4440515at2; -.
DR Proteomes; UP000000640; Chromosome.
DR GO; GO:0003988; F:acetyl-CoA C-acyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR42689; ACETYL-COA ACYLTRANSFERASE FADA2 (3-KETOACYL-COA THIOLASE) (BETA-KETOTHIOLASE)-RELATED; 1.
DR PANTHER; PTHR42689:SF1; ACETYL-COA ACYLTRANSFERASE FADA2 (3-KETOACYL-COA THIOLASE) (BETA-KETOTHIOLASE)-RELATED; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:ABL82349.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000640};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:ABL82349.1}.
FT DOMAIN 8..276
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 284..426
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 92
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 383
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 413
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 426 AA; 44916 MW; 9546950FDAE1224B CRC64;
MAANTRRVAV LGGNRIPFAR SNTVYAEASN QDMLTAAIDG LVERFGLQGE RLGEVVAGAV
LKHARDFNLT RESVLGSKLA PETPATDLQQ ACGTGLQAAI QVANKIALGQ IEAGIAGGTD
TTSDAPVAIS DRLRKKLMKV NAARDTKGKL LALGAIRPGD IGLEIPQNGE PRTRLSMGEH
AALTALEWQI GREEQDELAA ASHQHLAAAY DRGWLDDQIT PFRGVERDNN LRPDSTAEKL
AKLKPVFGKG EAATMTAGNS TPLTDGASAV LLASEEWADE HGLAPLAYFV DSETAAVDYV
HGGEGLLMAP AYAVPRMLAR NGLTLQDFDF YEIHEAFASQ VLATLKAWED PIFCKERLGL
DAPLGSIDRS KLNVNGSSLA AGHPFAATGG RILNVAAKLL AEKGSGRALI SICAAGGQGV
VAILER
//
