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Database: UniProt
Entry: A1UHM8_MYCSK
LinkDB: A1UHM8_MYCSK
Original site: A1UHM8_MYCSK 
ID   A1UHM8_MYCSK            Unreviewed;       580 AA.
AC   A1UHM8;
DT   06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2007, sequence version 1.
DT   12-SEP-2018, entry version 86.
DE   RecName: Full=Malto-oligosyltrehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE            Short=MTHase {ECO:0000256|PIRNR:PIRNR006337};
DE            EC=3.2.1.141 {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=Maltooligosyl trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
GN   OrderedLocusNames=Mkms_3142 {ECO:0000313|EMBL:ABL92336.1};
OS   Mycobacterium sp. (strain KMS).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=189918 {ECO:0000313|EMBL:ABL92336.1, ECO:0000313|Proteomes:UP000000638};
RN   [1] {ECO:0000313|EMBL:ABL92336.1, ECO:0000313|Proteomes:UP000000638}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KMS {ECO:0000313|EMBL:ABL92336.1,
RC   ECO:0000313|Proteomes:UP000000638};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Miller C.D., Richardson P.;
RT   "Complete sequence of chromosome of Mycobacterium sp. KMS.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of (1->4)-alpha-D-glucosidic
CC       linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to
CC       yield trehalose and (1->4)-alpha-D-glucan.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|PIRNR:PIRNR006337, ECO:0000256|SAAS:SAAS00964676}.
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DR   EMBL; CP000518; ABL92336.1; -; Genomic_DNA.
DR   RefSeq; WP_011560475.1; NC_008705.1.
DR   ProteinModelPortal; A1UHM8; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblBacteria; ABL92336; ABL92336; Mkms_3142.
DR   GeneID; 32419424; -.
DR   KEGG; mkm:Mkms_3142; -.
DR   HOGENOM; HOG000155669; -.
DR   KO; K01236; -.
DR   OMA; FTPMLFM; -.
DR   OrthoDB; POG091H0CAL; -.
DR   UniPathway; UPA00299; -.
DR   Proteomes; UP000000638; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033942; F:4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR022567; DUF3459.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR012768; Trehalose_TreZ.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF11941; DUF3459; 1.
DR   PIRSF; PIRSF006337; Trehalose_TreZ; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02402; trehalose_TreZ; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000000638};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR006337,
KW   ECO:0000313|EMBL:ABL92336.1};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR006337,
KW   ECO:0000313|EMBL:ABL92336.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000638}.
FT   DOMAIN       76    448       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    248    248       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   ACT_SITE    285    285       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   SITE        381    381       Transition state stabilizer.
FT                                {ECO:0000256|PIRSR:PIRSR006337-3}.
SQ   SEQUENCE   580 AA;  63917 MW;  BF90731B18B08ADC CRC64;
     MPEFAVWAPL PERVRLDVEG SLHPMTCGDD GWWRIEVDAA PDARYGFVLD DDPKVLPDPR
     SPRQPDGVHE RSQLWQPAPD AWTDGAWAGR KIEGSEKGQV IYELHTGTFT PEGTFDAAIG
     KLDHLVDLGV DFVELMPVNA FGGTHGWGYD GVLWYAVHEP YGGPDALIRL IDACHSRGLG
     VLIDAVFNHL GPSGNYLPKF GPYLSSGSNP WGESINIADA GADEVRRYIL DCALRWMRDF
     HADGLRLDAV HALVDTTAIH ILEELSAETD ALADELGRPL SLIAESDMND PRLITPRDQG
     GLGMTAQWDD DIHHAIHAAV SGETQGYYGD WGTVEALAQT LRNGYFHAGT FSSFRNRRHG
     RPLDTATIPA TRLLAYTLTH DQVGNRAVGD RPSQNLDTGQ LAVKAALALG SPYTAMLFMG
     EEWGSSSPFQ FFSSHPEPEL ARATAEGRKR EFAEHGWDAD EIPDPQDPQT FLRSKLKWDE
     IDDGDHGRLR ALYRELIALR HNEPDLADPW LDHLRIDYDE AQRWIVMHRG SLSIACNLGA
     DAVTVPVTGD VVLAWGEPAP EGDGMSLAGH SFAVVRTQSR
//
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