ID PDX1_GORGO Reviewed; 284 AA.
AC A1YF08;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=Pancreas/duodenum homeobox protein 1;
DE AltName: Full=Homeodomain protein PDX1;
DE AltName: Full=Insulin promoter factor 1;
DE Short=IPF-1;
GN Name=PDX1; Synonyms=IPF1;
OS Gorilla gorilla gorilla (Western lowland gorilla).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Gorilla.
OX NCBI_TaxID=9595;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Nickel G.C., Tefft D.L., Trevarthen K., Funt J., Adams M.D.;
RT "Positive selection in transcription factor genes on the human lineage.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Activates insulin and somatostatin gene transcription. Key
CC regulator of islet peptide hormone expression but also responsible for
CC the development of the pancreas, most probably by determining
CC maturation and differentiation of common pancreatic precursor cells in
CC the developing gut. Binds the DNA sequence 5'-CC[CT]TAATGGG-3' (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the basic helix-loop-helix domains of TCF3(E47)
CC and NEUROD1 and with HMG-I(Y). Interacts with SPOP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC Cytoplasm, cytosol {ECO:0000250}.
CC -!- DOMAIN: The Antp-type hexapeptide mediates heterodimerization with PBX
CC on a regulatory element of the somatostatin promoter. {ECO:0000250}.
CC -!- DOMAIN: The homeodomain, which contains the nuclear localization
CC signal, not only mediates DNA-binding, but also acts as a protein-
CC protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
CC {ECO:0000250}.
CC -!- PTM: Phosphorylated by the SAPK2 pathway at high intracellular glucose
CC concentration. Phosphorylated by HIPK2 on Ser-269 upon glucose
CC accumulation. This phosphorylation mediates subnuclear localization
CC shifting. Phosphorylation by PASK may lead to translocation into the
CC cytosol (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. IPF1/XlHbox-8
CC subfamily. {ECO:0000305}.
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DR EMBL; DQ976500; ABM46726.1; -; Genomic_DNA.
DR RefSeq; XP_018895105.1; XM_019039560.1.
DR AlphaFoldDB; A1YF08; -.
DR BMRB; A1YF08; -.
DR SMR; A1YF08; -.
DR STRING; 9593.ENSGGOP00000002529; -.
DR Ensembl; ENSGGOT00000002582.3; ENSGGOP00000002529.3; ENSGGOG00000002569.3.
DR GeneID; 101130338; -.
DR KEGG; ggo:101130338; -.
DR CTD; 3651; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000162542; -.
DR InParanoid; A1YF08; -.
DR OMA; HTWKGQW; -.
DR OrthoDB; 728401at2759; -.
DR Proteomes; UP000001519; Chromosome 13.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048565; P:digestive tract development; IEA:Ensembl.
DR GO; GO:0031017; P:exocrine pancreas development; IEA:Ensembl.
DR GO; GO:0042593; P:glucose homeostasis; IEA:Ensembl.
DR GO; GO:0006006; P:glucose metabolic process; IEA:Ensembl.
DR GO; GO:0030073; P:insulin secretion; IEA:Ensembl.
DR GO; GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IEA:Ensembl.
DR GO; GO:0001889; P:liver development; IEA:Ensembl.
DR GO; GO:0016331; P:morphogenesis of embryonic epithelium; IEA:Ensembl.
DR GO; GO:1902236; P:negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; IEA:Ensembl.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:2000675; P:negative regulation of type B pancreatic cell apoptotic process; IEA:Ensembl.
DR GO; GO:0007263; P:nitric oxide mediated signal transduction; IEA:Ensembl.
DR GO; GO:0032024; P:positive regulation of insulin secretion; ISS:UniProtKB.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:1904692; P:positive regulation of type B pancreatic cell proliferation; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0097050; P:type B pancreatic cell apoptotic process; IEA:Ensembl.
DR GO; GO:0003309; P:type B pancreatic cell differentiation; IBA:GO_Central.
DR GO; GO:0044342; P:type B pancreatic cell proliferation; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017995; Homeobox_antennapedia.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR PANTHER; PTHR45664:SF12; PANCREAS_DUODENUM HOMEOBOX PROTEIN 1; 1.
DR PANTHER; PTHR45664; PROTEIN ZERKNUELLT 1-RELATED; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00025; ANTENNAPEDIA.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..284
FT /note="Pancreas/duodenum homeobox protein 1"
FT /id="PRO_0000285452"
FT DNA_BIND 147..206
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 13..74
FT /note="Transactivation domain"
FT /evidence="ECO:0000250"
FT REGION 34..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 119..124
FT /note="Antp-type hexapeptide"
FT MOTIF 198..204
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 37..53
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..251
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 152
FT /note="Phosphothreonine; by PASK"
FT /evidence="ECO:0000250|UniProtKB:P52946"
FT MOD_RES 269
FT /note="Phosphoserine; by HIPK2"
FT /evidence="ECO:0000250|UniProtKB:P52945"
SQ SEQUENCE 284 AA; 30868 MW; 85A93DF1D080BAD8 CRC64;
MNGEEQYYAA TQLYKDPCAF QRGPAPEFSA SPPACLYMGR QPPPPPPPHP FPGALGALEQ
GSPPDISPYE VPPLADDPAV AHLHHHLPAQ LALPHPPAGP FPEGAEPGVL EEPNRVQLPF
PWMKSTKAHA WKGQWAGGAY AAEPEENKRT RTAYTRAQLL ELEKEFLFNK YISRPRRVEL
AVMLNLTERH IKIWFQNRRM KWKKEEDKKR GGGTAVGGGG VAEPEQDCAV TSGEELLALP
PPPPPGGAVP PAAPVAAREG RLPPGLSASP QPSSVAPRRP QEPR
//
