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Database: UniProt
Entry: A2XKG2
LinkDB: A2XKG2
Original site: A2XKG2 
ID   RH10_ORYSI              Reviewed;         472 AA.
AC   A2XKG2;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   31-JUL-2019, entry version 80.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 10 {ECO:0000305};
DE            EC=3.6.4.13 {ECO:0000269|PubMed:26848586};
DE   AltName: Full=DEAD-box RNA helicase TOGR1 {ECO:0000303|PubMed:26848586};
DE   AltName: Full=Protein THERMOTOLERANT GROWTH REQUIRED1 {ECO:0000303|PubMed:26848586};
GN   Name=RH10 {ECO:0000305}; Synonyms=TOGR1 {ECO:0000303|PubMed:26848586};
GN   ORFNames=OsI_12939 {ECO:0000312|EMBL:EAY91322.1};
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946 {ECO:0000312|EMBL:EAY91322.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, SUBUNIT,
RP   SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION
RP   PHENOTYPE, AND MUTAGENESIS OF GLY-47.
RC   STRAIN=cv. Zhongxian 3037 {ECO:0000303|PubMed:26848586};
RX   PubMed=26848586; DOI=10.1371/journal.pgen.1005844;
RA   Wang D., Qin B., Li X., Tang D., Zhang Y., Cheng Z., Xue Y.;
RT   "Nucleolar DEAD-box RNA helicase TOGR1 regulates thermotolerant growth
RT   as a pre-rRNA chaperone in rice.";
RL   PLoS Genet. 12:E1005844-E1005844(2016).
CC   -!- FUNCTION: Has ATP-dependent RNA helicase activity in vitro. Acts
CC       as a thermosensitive RNA chaperone required for normal processing
CC       of pre-rRNA intermediates. Required for normal cell division at
CC       high temperatures. Required for a primary metabolism adaptation to
CC       high temperatures to support thermotolerant growth by regulating
CC       gene expression. Partially rescues the yeast rRNA helicase RRP3
CC       mutant which has repressed cell proliferation at 38 degrees
CC       Celsius. {ECO:0000269|PubMed:26848586}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000269|PubMed:26848586};
CC   -!- ACTIVITY REGULATION: Helicase activity is activated by temperature
CC       increase. {ECO:0000269|PubMed:26848586}.
CC   -!- SUBUNIT: Interacts with U3 small nucleolar RNA (U3 snoRNA) as part
CC       of the ribosomal small subunit (SSU) processome.
CC       {ECO:0000269|PubMed:26848586}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26848586}.
CC       Nucleus, nucleolus {ECO:0000269|PubMed:26848586}.
CC       Note=Localization is not affected by temperature.
CC       {ECO:0000269|PubMed:26848586}.
CC   -!- DEVELOPMENTAL STAGE: Widely expressed in roots, shoots, leaves,
CC       culms, spikelets and anthers during different developing stages.
CC       {ECO:0000269|PubMed:26848586}.
CC   -!- INDUCTION: By high temperature and the circadian clock. Expression
CC       peaks at zeitgeber time 9 (ZT9) corresponding to afternoon of a
CC       subjective day, the hottest time of daily temperature fluctuation.
CC       {ECO:0000269|PubMed:26848586}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis. {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Thermosensitive dwarf mutant. Reduced plant
CC       height, root length and crown root number at 30, 32.5 and 35
CC       degrees Celsius, whereas they grow similarly to wild-type at 25
CC       degrees Celsius except for a slightly reduced plant height. At
CC       tillering stage, plants grown in a hot climate show narrowed leaf
CC       blades mainly due to a decrease in vascular bundle and lateral
CC       vein numbers, and all internodes are shortened. Furthermore,
CC       cross-sections of leaf sheath, leaf blades, internodes and root
CC       maturation zones do not show a significant difference in cell
CC       elongation, but a reduction in cell numbers compared to wild-type.
CC       {ECO:0000269|PubMed:26848586}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX47/RRP3
CC       subfamily. {ECO:0000305}.
DR   EMBL; CM000128; EAY91322.1; -; Genomic_DNA.
DR   SMR; A2XKG2; -.
DR   EnsemblPlants; BGIOSGA010088-TA; BGIOSGA010088-PA; BGIOSGA010088.
DR   Gramene; BGIOSGA010088-TA; BGIOSGA010088-PA; BGIOSGA010088.
DR   eggNOG; KOG0330; Eukaryota.
DR   eggNOG; ENOG410XQU7; LUCA.
DR   HOGENOM; HOG000268802; -.
DR   OMA; KAKNRSI; -.
DR   Proteomes; UP000007015; Chromosome 3.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; RNA-binding;
KW   rRNA processing; Stress response.
FT   CHAIN         1    472       DEAD-box ATP-dependent RNA helicase 10.
FT                                /FTId=PRO_0000443718.
FT   DOMAIN       72    245       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      256    416       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      85     92       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        41     69       Q motif. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00552}.
FT   MOTIF       193    196       DEAD box. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MUTAGEN      47     47       G->V: In togr1-1; thermosensitive dwarf
FT                                mutant. {ECO:0000269|PubMed:26848586}.
SQ   SEQUENCE   472 AA;  52480 MW;  019998F2508FB19B CRC64;
     MAKKKDVEVE ELDEEVVAAA AAPAADGGEE QEAEPPARRP STFAELGVVP ELVAACDAMG
     WKEPTRIQAE AIPHALEGRD LIGLGQTGSG KTGAFALPII QALLKQDKPQ ALFACVLSPT
     RELAFQIGQQ FEALGSAIGL SCTVLVGGVD RVQQAVSLAK RPHIVVGTPG RLLDHLTDTK
     GFSLNKLKYL VLDEADKLLN VEFQKALDDI LNVIPKERRT FLFSATMTNK VSKLQRACLR
     NPVKVEVASK YSTVDTLRQE FYFVPADYKD CFLVHVLNEL PGSMIMIFVR TCESTRLLAL
     TLRNLRFKAI SISGQMSQDK RLGALNRFKT KDCNILICTD VASRGLDIQG VDVVINYDIP
     MNSKDYVHRV GRTARAGNTG YAVSLVNQYE AMWFKMIEKL LGYEIPDRKV DNAEIMILRE
     RISDSKRIAL TTMKEGGGHK KKRRKNEDDE EEEERNAPVS RKSKSFNKSR RR
//
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