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Database: UniProt
Entry: A3AZ88
LinkDB: A3AZ88
Original site: A3AZ88 
ID   MT2C_ORYSJ              Reviewed;          84 AA.
AC   A3AZ88; P93433; Q0DLB2; Q65XV5;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   10-APR-2019, entry version 55.
DE   RecName: Full=Metallothionein-like protein 2C;
DE   AltName: Full=Class I metallothionein-like protein 2C;
DE   AltName: Full=OsMT-I-2c;
DE   AltName: Full=OsMT2b;
GN   Name=MT2C; Synonyms=MTE;
GN   OrderedLocusNames=Os05g0111300, LOC_Os05g02070;
GN   ORFNames=OsJ_016110, P0016H04.13;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Lomello;
RA   Chen P.-W., Chen L.-J.;
RT   "Isolation of a cDNA clone encoding a type 2 metallothionein-like
RT   protein from rice embryo.";
RL   (er) Plant Gene Register PGR97-109(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S.,
RA   Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=15220467; DOI=10.1104/pp.103.036384;
RA   Wong H.L., Sakamoto T., Kawasaki T., Umemura K., Shimamoto K.;
RT   "Down-regulation of metallothionein, a reactive oxygen scavenger, by
RT   the small GTPase OsRac1 in rice.";
RL   Plant Physiol. 135:1447-1456(2004).
RN   [8]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   PubMed=17002881; DOI=10.5483/BMBRep.2006.39.5.595;
RA   Zhou G., Xu Y., Li J., Yang L., Liu J.-Y.;
RT   "Molecular analyses of the metallothionein gene family in rice (Oryza
RT   sativa L.).";
RL   J. Biochem. Mol. Biol. 39:595-606(2006).
RN   [9]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=18258694; DOI=10.1104/pp.107.110304;
RA   Yuan J., Chen D., Ren Y., Zhang X., Zhao J.;
RT   "Characteristic and expression analysis of a metallothionein gene,
RT   OsMT2b, down-regulated by cytokinin suggests functions in root
RT   development and seed embryo germination of rice.";
RL   Plant Physiol. 146:1637-1650(2008).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine
CC       residues that bind various heavy metals (Probable). Acts as
CC       reactive oxygen species (ROS) scavenger in the cytosol. Possesses
CC       superoxide anion and hydroxyl radical scavenging activities in
CC       vitro (PubMed:15220467). Plays a role during root development,
CC       lateral root initiation and seed embryo germination, possibly by
CC       regulating levels of cytokinin (PubMed:18258694).
CC       {ECO:0000269|PubMed:15220467, ECO:0000269|PubMed:18258694,
CC       ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000269|PubMed:15220467}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in inflorescences. Expressed
CC       in roots and leaves (PubMed:15220467, PubMed:17002881). Expressed
CC       in root primordium, pericycle, phloem and in the basal parts of
CC       lateral roots (PubMed:18258694). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:17002881, ECO:0000269|PubMed:18258694}.
CC   -!- DEVELOPMENTAL STAGE: During embryo development, expressed in the
CC       top of the scutellum at 7 days after pollination (DAP). In the
CC       germinating embryos, expressed in the scutellum and the cap of the
CC       radicle at 2 days after germination (DAG). At 4 DAG, still
CC       expressed in scutellum, and appears in the vascular bundle of
CC       shoot. In florets of young panicle, expressed in the pedicel and
CC       the basement of glumes. In florets of older panicle, expressed in
CC       the pedicel, basement of glumes, anthers and pistils. Expressed in
CC       the basal parts of ovaries before pollination and in the basal
CC       parts of stigmas and ovaries after pollination.
CC       {ECO:0000269|PubMed:18258694}.
CC   -!- INDUCTION: By Fe, Zn and auxin in roots, and Cu and Mn in shoots.
CC       Down-regulated by cytokinin and cold stress in roots and shoots.
CC       Down-regulated by Cu and salt stress in roots (PubMed:18258694).
CC       Sinergically down-regulated by RAC1 and sphingolipid elicitor
CC       (PubMed:15220467). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:18258694}.
CC   -!- MISCELLANEOUS: Down-regulation by RAC1 during pathogen infection
CC       may potentiate ROS which function as signals in resistance
CC       response (PubMed:15220467). Plants silencing MT2C show abnormal
CC       plant growth and root formation (PubMed:18258694).
CC       {ECO:0000269|PubMed:15220467, ECO:0000269|PubMed:18258694}.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15
CC       family. {ECO:0000305}.
DR   EMBL; U57638; AAD10192.1; -; mRNA.
DR   EMBL; AF048750; AAD02475.1; -; Genomic_DNA.
DR   EMBL; AC079356; AAU44207.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF16361.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS91926.1; -; Genomic_DNA.
DR   EMBL; CM000142; EAZ32627.1; -; Genomic_DNA.
DR   RefSeq; XP_015638726.1; XM_015783240.1.
DR   UniGene; Os.100198; -.
DR   UniGene; Os.18500; -.
DR   UniGene; Os.73772; -.
DR   UniGene; Os.74845; -.
DR   ProteinModelPortal; A3AZ88; -.
DR   STRING; 4530.OS05T0111300-01; -.
DR   EnsemblPlants; Os05t0111300-01; Os05t0111300-01; Os05g0111300.
DR   GeneID; 4337596; -.
DR   Gramene; Os05t0111300-01; Os05t0111300-01; Os05g0111300.
DR   KEGG; osa:4337596; -.
DR   InParanoid; A3AZ88; -.
DR   OMA; MVIAEAT; -.
DR   OrthoDB; 1648303at2759; -.
DR   Proteomes; UP000059680; Chromosome 5.
DR   ExpressionAtlas; A3AZ88; differential.
DR   Genevisible; A3AZ88; OS.
DR   GO; GO:0005829; C:cytosol; IMP:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0072593; P:reactive oxygen species metabolic process; IMP:UniProtKB.
DR   InterPro; IPR000347; Metalthion_15p.
DR   PANTHER; PTHR33543; PTHR33543; 1.
DR   Pfam; PF01439; Metallothio_2; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; Metal-binding; Metal-thiolate cluster;
KW   Reference proteome; Stress response.
FT   CHAIN         1     84       Metallothionein-like protein 2C.
FT                                /FTId=PRO_0000197408.
SQ   SEQUENCE   84 AA;  7969 MW;  5BF71575A9142311 CRC64;
     MSCCGGNCGC GSGCQCGGGC GGCKMFPDVE ATATTKTFVL AAPSNKASSG GMEMAVESGE
     NGGCGCNTCK CGTSCSGCSC CSCN
//
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