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Database: UniProt
Entry: A3LSJ2
LinkDB: A3LSJ2
Original site: A3LSJ2 
ID   DBP6_PICST              Reviewed;         591 AA.
AC   A3LSJ2;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   16-OCT-2019, entry version 68.
DE   RecName: Full=ATP-dependent RNA helicase DBP6;
DE            EC=3.6.4.13;
GN   Name=DBP6; ORFNames=PICST_35694;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 /
OS   NRRL Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Debaryomycetaceae;
OC   Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-
RT   fermenting yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of
CC       60S ribosomal subunits and is required for the normal formation of
CC       25S and 5.8S rRNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Associated with pre-ribosomal particles. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX51/DBP6
CC       subfamily. {ECO:0000305}.
DR   EMBL; CP000497; ABN65577.2; -; Genomic_DNA.
DR   RefSeq; XP_001383606.2; XM_001383569.1.
DR   SMR; A3LSJ2; -.
DR   STRING; 4924.XP_001383606.2; -.
DR   EnsemblFungi; ABN65577; ABN65577; PICST_35694.
DR   GeneID; 4837855; -.
DR   KEGG; pic:PICST_35694; -.
DR   eggNOG; KOG0350; Eukaryota.
DR   eggNOG; ENOG410XRWM; LUCA.
DR   HOGENOM; HOG000203294; -.
DR   InParanoid; A3LSJ2; -.
DR   KO; K14807; -.
DR   OMA; ATMTRDI; -.
DR   OrthoDB; 973872at2759; -.
DR   Proteomes; UP000002258; Chromosome 3.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030687; C:preribosome, large subunit precursor; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    591       ATP-dependent RNA helicase DBP6.
FT                                /FTId=PRO_0000285152.
FT   DOMAIN      195    377       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      414    570       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     208    215       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       153    181       Q motif.
FT   MOTIF       315    318       DEAD box.
SQ   SEQUENCE   591 AA;  66322 MW;  77ED0F3F5DBEA3AF CRC64;
     MFGARFDPTA DDSSSDESEE ESEEEENENE NVVNNDVAKD IDSVESEESE DSDHSNDPND
     AMDVDSEVNT EVDVDYVSKY KSVFDKFKKS VPETKKIDSE SSSEEEDDVE MQDLMPLPQP
     ALPRDKRLIS SNSHLKNLDW LATPIYASPE DSKPFSEFEI SPFLLKNLER DNFTTAFSVQ
     IAIMDILLHD IKRNRLEPDV KGDILVNAAT GSGKTLAYSI PIIEALHNRV VPRVRAIVLV
     PTKPLINQVK ATFVQLSRGT NLSVVSLRND VSIKEEGIKI VNSPPDIIVS TPGRLVEHIS
     NKSINLNSLQ FLVIDEADRL LNQSFQNWCQ VLISSLEGDV NIAEEWKITP QKLIFSATLT
     TDSGKLSALK FQKPRLVIVN DRKQLVNEIF NVPSSLSEYT IQFGTAKASI KPLILAKYLL
     ENNKLSNVLI FTKSNEASIR LCKLLELMFG KLHPSMNIAY INSTNNKSAI RTKILKDFST
     QKINILVATD LIARGIDILS ITDVINYDLP NSSREYVHRV GRTARANQTG HAYTLCFGKG
     EAKWFKKIIS DVGRGDKTIE KVEIELKELI VAEDENVYNE CLESLSKQVF H
//
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