ID A3SRD9_ROSNI Unreviewed; 492 AA.
AC A3SRD9;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=ISM_11260 {ECO:0000313|EMBL:EAP75162.1};
OS Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Roseovarius.
OX NCBI_TaxID=89187 {ECO:0000313|EMBL:EAP75162.1, ECO:0000313|Proteomes:UP000005954};
RN [1] {ECO:0000313|EMBL:EAP75162.1, ECO:0000313|Proteomes:UP000005954}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-591 / DSM 15170 / ISM
RC {ECO:0000313|Proteomes:UP000005954};
RA Moran M.A., Ferriera S., Johnson J., Kravitz S., Halpern A., Remington K.,
RA Beeson K., Tran B., Rogers Y.-H., Friedman R., Venter J.C.;
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAP75162.1}.
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DR EMBL; AALY01000004; EAP75162.1; -; Genomic_DNA.
DR RefSeq; WP_009814261.1; NZ_CH724156.1.
DR AlphaFoldDB; A3SRD9; -.
DR STRING; 89187.ISM_11260; -.
DR eggNOG; COG0389; Bacteria.
DR HOGENOM; CLU_028184_1_1_5; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000005954; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000005954}.
FT DOMAIN 37..154
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 240..340
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
SQ SEQUENCE 492 AA; 54859 MW; 323014633A14D23E CRC64;
MFDGLQRRVV SIWFPRLASD RVLRLRPIAG PFVLTLREAN AERLHCLNQA AERQGLHRGM
SFADARAFCP DLQSRPADLQ AEARFLHVLR RWATRYCPWV GLEGDDGLVL DITGSAHLFG
GESQMLANMR QGLGRAGVAA RLGLGDTRGA AWALARHGEG CAAPGAPQAA LADLPVAALR
LDPDLVTGLQ RLGLRRIADL DATPRAPLAR RFGPAPLLRL DQALGRQPEE ITPLAEPPHY
GVRMTLPEPI GLCDDVMAGV SRLLDQLCAK LAHHETGARS LCLTLRRVDQ EARTVELRLA
QPLRDAARIL PLFERGVAAV EAGYGIDQLR LEAVQVERLP ARQITHMEGR DDSQLDDLIT
RIGTRIGLEN VQRFLPADSH IPERGFLISP AAYSRPEGNW SAPRPRPLVL FPPEPIAGHG
PRPPARFRWR RMALSVARLC GPERIAPEWW LSDDNWRRGL RDYWQVQTHQ GRRLWMFYTP
QAPGWFVQGE FA
//
