ID A3TKY3_9MICO Unreviewed; 495 AA.
AC A3TKY3;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 13-SEP-2023, entry version 54.
DE RecName: Full=Glutamate--cysteine ligase {ECO:0008006|Google:ProtNLM};
GN ORFNames=JNB_02680 {ECO:0000313|EMBL:EAP99038.1};
OS Janibacter sp. HTCC2649.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Janibacter.
OX NCBI_TaxID=313589 {ECO:0000313|EMBL:EAP99038.1, ECO:0000313|Proteomes:UP000005063};
RN [1] {ECO:0000313|EMBL:EAP99038.1, ECO:0000313|Proteomes:UP000005063}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC2649 {ECO:0000313|EMBL:EAP99038.1,
RC ECO:0000313|Proteomes:UP000005063};
RX PubMed=21075932; DOI=10.1128/JB.01298-10;
RA Thrash J.C., Cho J.C., Bertagnolli A.D., Ferriera S., Johnson J.,
RA Vergin K.L., Giovannoni S.J.;
RT "Genome sequence of the Marine Janibacter Sp. Strain HTCC2649.";
RL J. Bacteriol. 193:584-585(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029283};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAP99038.1}.
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DR EMBL; AAMN01000002; EAP99038.1; -; Genomic_DNA.
DR AlphaFoldDB; A3TKY3; -.
DR STRING; 313589.JNB_02680; -.
DR eggNOG; COG2170; Bacteria.
DR HOGENOM; CLU_029030_0_0_11; -.
DR Proteomes; UP000005063; Unassembled WGS sequence.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:InterPro.
DR GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.590.20; -; 1.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR016602; UCP012666.
DR PANTHER; PTHR36510:SF3; CONSERVED PROTEIN; 1.
DR PANTHER; PTHR36510; GLUTAMATE--CYSTEINE LIGASE 2-RELATED; 1.
DR Pfam; PF04107; GCS2; 1.
DR PIRSF; PIRSF012666; UCP012666; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000005063}.
SQ SEQUENCE 495 AA; 56031 MW; 554260609C522247 CRC64;
MGVGAEVSAQ SYTREERQRY REKVRQNLDV FERMLNQHSF EFDRPLTGLE IELNLVNSDF
DPHFANAEVL QAIADPGYQT ELAKYNIELN VLPRPLPGDS AIELEHDLRE SLNAADAKAR
EVGSRLVAIG ILPTIMPEHY EGEWISDNNR YTALNDSIFV ARGEDIFLDI TGPQGERVST
YCDSIAPESA CTSVQLHLQV SPSDFADHWN AAQAVSPVQV ALAANSPFFF GKRLHAETRI
ELFKQATDTR PIELKNQGVR PRVFFGERWI TSIFDLFEEN SRYFPALLAE TTDEDPMAKL
EAGEAPDLAE LRLHNGTVYR WNRPIYDIVK GRPHLRVENR VLPAGPTIAD TLANAAFYYG
VVRKLASTER PVWTKMSFAA CEENFHRGAR DGMEARLYWP GFGEVGADEL VLRHLLPLAH
EGLEEWGVSQ AVRDRFLGII EARCQAGVNG ATWQTDAVRR FEERGSDRRT ALHQMLELYT
EHMSTNEPVH TWPLP
//
