ID A3TNH4_9MICO Unreviewed; 1243 AA.
AC A3TNH4;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 08-NOV-2023, entry version 93.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=JNB_13998 {ECO:0000313|EMBL:EAP98082.1};
OS Janibacter sp. HTCC2649.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Janibacter.
OX NCBI_TaxID=313589 {ECO:0000313|EMBL:EAP98082.1, ECO:0000313|Proteomes:UP000005063};
RN [1] {ECO:0000313|EMBL:EAP98082.1, ECO:0000313|Proteomes:UP000005063}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC2649 {ECO:0000313|EMBL:EAP98082.1,
RC ECO:0000313|Proteomes:UP000005063};
RX PubMed=21075932; DOI=10.1128/JB.01298-10;
RA Thrash J.C., Cho J.C., Bertagnolli A.D., Ferriera S., Johnson J.,
RA Vergin K.L., Giovannoni S.J.;
RT "Genome sequence of the Marine Janibacter Sp. Strain HTCC2649.";
RL J. Bacteriol. 193:584-585(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAP98082.1}.
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DR EMBL; AAMN01000003; EAP98082.1; -; Genomic_DNA.
DR RefSeq; WP_009777087.1; NZ_CH672413.1.
DR AlphaFoldDB; A3TNH4; -.
DR STRING; 313589.JNB_13998; -.
DR eggNOG; COG5013; Bacteria.
DR HOGENOM; CLU_000422_14_1_11; -.
DR Proteomes; UP000005063; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000005063}.
FT DOMAIN 52..116
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
FT REGION 1000..1023
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1243 AA; 136982 MW; C451D02476E7767A CRC64;
MSGIAMDGSF TESLVRSRRF FTKGTVSDDL RTISQVGGRE ADDFYRDRWS HDKVVRSTHG
VNCTGSCSWK VYVKDGIITW ETQQTDYPSV GPDSPEYEPR GCPRGAAFSW YTYSPTRVRY
PYVRGVLLQM FREAKAQHDG DPVRAWEHIV ENPLRAKAYK SARGKGGLVR ATWDEASEII
AAAHVHTIKK YGPDRIAGFS PIPAMSMVSH ASGARFVNLI GGSMLSFYDW YADLPVASPQ
VFGDQTDVPE SGDWWNAGYL IMWGSNLPVT RTPDAHWMVE ARYRGQKVIA VAPDYADNVK
FADEWLAAKP GTDAALAMAM GHVVLREFFV DRQTPYFTEY VKSFTDLPHL VRLEQSADGE
YTAGKFLTAA DLVDHCDEEN AAFKTVLIDS TTGEAVVPNG SLGHRYGEAG VGKWNLDLGE
VSPVLSLLDS ASESVVVRMP RFDTPDGAAA DLPRGVPVRL VDGHLVTTVF DLLLAQYGVG
RSHHGQPLPG AWPTGDNKGY DDAQAPYTPA WQEAITGVPA ATAARIGREF AQNAEESKGR
SMIVMGAGTN HWFHSDTIYR AFLTLTNLTG CQGVNGGGWA HYVGQEKVRP ITGYTQIANA
LDWNRPPRNM IQTAYWYLHT NQFRYDQFGA DTLSATTGKG QLAGRSTADV IAQSARMGWM
PSYPTFDRNP LDLSDDAAAA GKPVGEYVVE QLKSGELDFA GEDPDAPTNY PRILSIWRAN
LLGSSGKGNE YFLKHLLGTD SSVRATETPE AQRPVDVTWR DEAPEGKLDL LMTIDFRQTS
TTIFSDVVLP AATWYEKHDL NTTDMHPFVH SFNPAIAPPW QTRTDWDAWQ TIAEKFSELA
ATHLGVRKDV VAVPLTHDTP DAMANPHGVV RDWKKGECEP IPGVTMPKLV EVERDYAAVA
QKMNALGPLV DTLGATTKGV TFELSKQVDY LRAKNGTVRG GVADGRPSLK KDVNVCEAIL
ALSGTTNGHL ATQGFRTLEK RTGVQLADLA QEHEGKQITF ADTQGPPVPV ITSPEWSGSE
TGGRRYSPFT INVERKKPWH TLSGRMHFYL DHDWMTELGE GLPVYRPPLN MAALFAEPAL
GTVSDGSAGQ VEGLTVRYLT PHNKWSIHSE YQDNLFMLSL SRGGQNIWMS DRDAAKVGIA
DNDWIEAVNR NGVVVARAIV SHRMPEGTVY MYHAQDRLID VPLAETSGKR GGIHNSLTRL
LVKPSHLIGG YAQLTFAFNY LGPTGNQRDE VTIIRKRSQD VTY
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