ID A3VBP6_9RHOB Unreviewed; 549 AA.
AC A3VBP6;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=RB2654_16956 {ECO:0000313|EMBL:EAQ14379.1};
OS Maritimibacter alkaliphilus HTCC2654.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Maritimibacter.
OX NCBI_TaxID=314271 {ECO:0000313|EMBL:EAQ14379.1, ECO:0000313|Proteomes:UP000002931};
RN [1] {ECO:0000313|EMBL:EAQ14379.1, ECO:0000313|Proteomes:UP000002931}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC2654 {ECO:0000313|EMBL:EAQ14379.1,
RC ECO:0000313|Proteomes:UP000002931};
RX PubMed=20729358; DOI=10.1128/JB.00873-10;
RA Thrash J.C., Cho J.C., Ferriera S., Johnson J., Vergin K.L.,
RA Giovannoni S.J.;
RT "Genome sequences of Pelagibaca bermudensis HTCC2601T and Maritimibacter
RT alkaliphilus HTCC2654T, the type strains of two marine Roseobacter
RT genera.";
RL J. Bacteriol. 192:5552-5553(2010).
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAQ14379.1}.
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DR EMBL; AAMT01000002; EAQ14379.1; -; Genomic_DNA.
DR RefSeq; WP_008333765.1; NZ_VNHV01000004.1.
DR AlphaFoldDB; A3VBP6; -.
DR STRING; 314271.RB2654_16956; -.
DR eggNOG; COG0389; Bacteria.
DR HOGENOM; CLU_028184_1_1_5; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000002931; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR045443; DUF6504.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF20114; DUF6504; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50173; UMUC; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002931}.
FT DOMAIN 15..150
FT /note="UmuC"
FT /evidence="ECO:0000259|PROSITE:PS50173"
FT REGION 171..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 549 AA; 59940 MW; F5D20D50131481E0 CRC64;
MPAKRHLSLW FPRLAAERLL RLDRGLPAGP FVVVEDQRGA QVIASMNAEA QAAGLSLGQP
LRDGRAMCPG LTSRPANPVA EAAFLTTLRR WAGRFSPWVS EEAPDGLILD ITGCAHLFGG
EDELAAEIDR ECTTLGLTVR LGLADTLGAA WALARFANVA GSGHRSGDAI DQEARATRSR
AVKRKHWTRG GTAPKPATQS VAPRIAPVGQ TRQAIGPLPV AALRLETETV EGLTRLGLRR
IDDLFGTPRA GLARRFGRHM VRRLDQALGV EPEPVSPARP PVRFAVRMGL PDPIGLEEDL
LACFDRLLPE LCDRLTRAGR GARRVRVEFS RTDHTMQAVE IGLARPTADP HRMRPLLGMK
LSDVDAGFGI DAVRLVVTSH EPVHATQHRG HLEAGATVSN RGPGAALDDL LGRIGTRLGL
EAMVRLHPAE SHIPEKGATV LAAAFSQPAT NWQAPARERP LRMWPPEPVM IPDLPHLPET
FRWRGRDHGV ASAFGPERLA PEWWLDDPAW RTGQRDYWVV TTTRGERLWL YFAHGAAQSA
GWFCHGAFA
//
