ID A4FDM8_SACEN Unreviewed; 4132 AA.
AC A4FDM8;
DT 17-APR-2007, integrated into UniProtKB/TrEMBL.
DT 17-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE SubName: Full=Ansamitocin polyketide synthase A {ECO:0000313|EMBL:PFG95888.1};
DE SubName: Full=Modular polyketide synthase {ECO:0000313|EMBL:CAM02153.1};
GN OrderedLocusNames=SACE_2875 {ECO:0000313|EMBL:CAM02153.1};
GN ORFNames=A8924_3267 {ECO:0000313|EMBL:PFG95888.1};
OS Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharopolyspora.
OX NCBI_TaxID=405948 {ECO:0000313|EMBL:CAM02153.1, ECO:0000313|Proteomes:UP000006728};
RN [1] {ECO:0000313|EMBL:CAM02153.1, ECO:0000313|Proteomes:UP000006728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC {ECO:0000313|EMBL:CAM02153.1};
RX PubMed=17369815; DOI=10.1038/nbt1297;
RA Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA Haydock S.F., Leadlay P.F.;
RT "Complete genome sequence of the erythromycin-producing bacterium
RT Saccharopolyspora erythraea NRRL23338.";
RL Nat. Biotechnol. 25:447-453(2007).
RN [2] {ECO:0000313|EMBL:PFG95888.1, ECO:0000313|Proteomes:UP000225825}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG95888.1,
RC ECO:0000313|Proteomes:UP000225825};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
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DR EMBL; AM420293; CAM02153.1; -; Genomic_DNA.
DR EMBL; PDBV01000001; PFG95888.1; -; Genomic_DNA.
DR RefSeq; WP_009946373.1; NZ_PDBV01000001.1.
DR STRING; 405948.SACE_2875; -.
DR KEGG; sen:SACE_2875; -.
DR eggNOG; COG0318; Bacteria.
DR eggNOG; COG0604; Bacteria.
DR eggNOG; COG1086; Bacteria.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000086_0_0_11; -.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000006728; Chromosome.
DR Proteomes; UP000225825; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05195; enoyl_red; 1.
DR CDD; cd08956; KR_3_FAS_SDR_x; 2.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 3.40.50.11460; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF13602; ADH_zinc_N_2; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 5.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000006728};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 970..1048
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1067..1493
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3034..3109
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3132..3556
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4052..4126
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 3561..3583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4132 AA; 435358 MW; 69069AC667062AA5 CRC64;
MLRTELIRPL PELLRSHASR LEDKIAFRDS RLSVSYADLE LRTRRLAGHL ADLRLQPGDR
AAILLGNRVE VVESYLAILR AGAIGVPLNP RVTETELSYL LEDSGARVVI TDEAHVEQVR
AAGGEVRRIV VVGDGPVPSG TVSYAHLAAT DPATPARDDL PLDAPAWMLY TSGTTGRPKG
VLSSQRNCLW SVAACYVPIP GLSEEDRVLW PLPLFHSLSH IACVLGVTAV GATARVLDGF
SADEVLNALR EDSTTFLAGV PTMYHHLVRA AGEQRLSLPD LRMCLVGGAI TTAALRRSFE
QAFGAPLLDA YGSTETCGSI TINWPTGARV EGSCGLPVPG LGVRLVDPET LVDVATGEEG
EVWVQGPSVM AGYHDQPEAT AAVFHDGWYR TGDLARRDES GYFTISGRIK ELIIRGGENI
HPGDVEAVLR SVPGVADAAV VGKPHEVLGE VPVAFLVPGP EGLDPEHVLA VCRQELSYIK
VPEELYEIDR VPRTASGKIT RHVLLDRPAR LRAAGGARHE LLFQLDWLPL PTVPASRSAA
RHWAVVGPDV FGVADSLDSQ GRSVDRYSDA DELVESVDSA EAAPEVTVLQ VAGTTAGGAA
SPAEEARDRV RVVTEQAESW LGDERFAEGT LVVVTRAAVA AGPDDEVSDL VAAPVWGAVR
ALRAAHPGRV VLVDLDDGGS AAALAEAVSS AEPELLLRSG VALRPRANRV AATGGSRRPF
QLTSRDTALV AGADGVVAAT VARHLAAVHD VRRFLLVSRG GDADLAAREL RAELTAAGAS
VALVACDVAD RDALAGLLAG HRPAAVVFAA GAGREALSTM DGALNLHELT LDAGLKAFVL
LSSAAGTLGG AGSADDAAAG VFLDAVARHR AARGLPALSV VFGPGEHEDW SVPAGVGRLT
GREHAAMFDA AHLADRTSVL ALRPDSTAVG SGSLPPALRD LIDVPVQADA PDDGTVSELR
ERLAPLSRDE QDRTLVELVR GEVARLYAMP DGATVPSDRA FRDLGLDSAT AVQLRNRLTA
TTGLDLPLTL AFDHPTPARV GRHLRSLLFG GERAKAGADA RVVSHSGEPI AIVAMGCRLP
GGVSSPEELW DLVAGEGDAV GDFPGDRGWN LEELFDPDPG RAGKSYVRHG GFLDDVAGFD
PEFFGISPRE AEAMDPQQRL LLEVSWEVLE RAAIAPDSLR GADVGVFSGV MYHDYASDLG
PVPEGLEGYL GTGNSGSVAS GRVSYTLGLQ GPAVTVDTAC SSSLVALHVA AQALRDGECE
LALAGGVAVM AKPTSFVEFS RQRALAPDGR CKAFAEAADG TAWAEGVGVL VLERLSQARR
NGHQVLAVVR GSAVNQDGAS NGLTAPSGPA QERVIRRALA SAGLSAAEVD AVEAHGTGTR
LGDPIEAQAL LATYGQGRPA GHPLWLGSLK SNIGHAQAAA GVAGVIKVVQ AMRHGVLPRT
LHVDEPSSRI DWSAGAVELL REARPWPELD RPRRAAVSSF GVSGTNAHVI LEHADEPDAA
PEEAGSTAPV PWVLSARTAE GLRAQAGRWA SFLADRPGHR PADVGFSSVT TRAALEHRAA
VVATDPADAV AALNAFAAGE RPQEVVADSA GVEGKLAMVF PGQGAQWAGM GAALLDSSPV
FAARIAECEQ ALQPHVDWSL RDVLRQREGA PSLERVDVVQ PVSFAVMVSL AELWRSYGIA
PDAVVGHSQG EIAAACVAGA LSIEDAAKVV ALRSQAIRRG LAGRGGMMSV SLPLDRATDR
LRPGLEVAAV NGPTSVVVAG EPGALEELLA ECEAEDVRAR RVPVDYASHT SHVERIREEL
LEVLADVRPR TADVPFFSTV DGRWLDTAAL DGEYWYRNLR RTVGFAAAVD TLAEQGYRAF
VEASSHPVLT TSVQEGLDQR QQPVTAVVTG SLRRDDGGLD RFLASLTRLW AAGVPIDWSA
VFEGTGGRRV ELPTQAFQHR RYWLEASERT GDVSTAGLEE ADHPLVGAVV SLPESGGVLC
TGRLSMRTHP WLADHAVSGT PIVPGAALVE LAVRAGDQVG FGVVEELVIE QPLLLPATGG
VRLQVSVSGV DDGGRRQVAI HSRADDGPSD AWSRHATGFL AATGEAPQES SEQWPPRDAD
PVDVTGFYEN QAAAGYGYGP AFQGVRAAWA RDEEFFAEVS LADAHVAEAQ RFGLHPALLD
AALHSVKLRH DAEGELRLPF AFRGVRLHAS GASQLRVRAV ASGPDEITVG LADPLGHPVA
SIESLVLRPA PDDELATGRS AQRAPMYRTA WTPVPQADGD AATASAVLGT GWGPAELPEH
ESVDALVRAL DSGLPVPEVV LARVEPEADD TDPARVRAVA AEVLERVQRW LAEPVLEPSH
LVVLTRGAVP VHHADEVRDL AAAAVWGLVR SAQSENPGRI TIVDADRLDG RLPPLPADEP
QLALRDGTFH APRLATHSGE LEVPGRSWRL EVTDGGDSPA DLALVALPED PAPLAPGEVR
LAVRAAGVNF KDVLITLDML GVRHGLGGEA AGVVLEVGDG VTDLAPGDRV MGVVGDYGAF
ATEAVTDRRL LARIPRGWSF EQAASVPVVF LTAYYGLRDL AGLSAGQSVL VHAAAGGVGM
AATQLARHWG AEVFGTAGPG KWDALRSLGL TDDHIASSRT AEFGELFRER TGGRGVDVVL
NSLTGELAET SLALLPRGGH FLELGKTETR DAGQVAEAHP GVRYQAYALY EAGPERTGQM
LADLVELFES GAITPSPLSV WDVRRAPEAF RHISQARHVG KVVLSVPRAP EPGGTVLITG
GTGTLGAETA RHLVAEHGVR NLVLCGRQGM AAPGAPELAE ELTRLGARVS IESCDVADRE
SLARLLAAVP PEHPLTGVVH TAGALDDGVI SALDEGRIAT VFRPKVDAAL HLHELTADLD
LAWFVLFSSA SGVFGNPGQG NYAAANAFMD GLAQRRRAAG LPAVSLAWGW WAQTSALTAA
LDDTAAARHR RNGLQPMPTE TGMDLLDSGL DAGHAALVPV SLDLPALRAR ASSEPVPPLL
RGLVRPGRRV ASRAATASDL AGRLANAPAA EQTRMLLELV CKEAAAVLGH ATSDAIDPNR
PFKEAGFDSL TAVEVRNRLN DATKLRLSTT VVFDYPTPAV LAEHIRVQVV GEDAESEKAP
VATAATGGGL SEDPIAIVSI GCRFPGAAEG PEEFWRVLRD EVDVMTDLPD DRGWNHHAFD
PDPDAPGSFY TRRGAFLDAA AFDAEFFGIS PREAVAMDPQ QRLLLEVAWE TVERAGIDPT
ALRGAGVGVF TGVINHDYTK LFVHPPAELE GHLMTGTAGS VASGRIAYTL GLRGPALTVD
TACSSSLVAL HLAMHSLRRG ECELAIAGGA TLMATLDNYV EFSRHRGLAP DGRCKAFAAA
ADGTAWSEGA GLLLLERLSD ARRNGHEVLA VLRGSAVNHD GASNGLTAPN GPAQQDVIRQ
ALADAGLSTS DVDVVEAHGT GTRLGDPIEA DALLATYGRD READRPLWLG SVKSNIGHSQ
AAAGVAGVIK VVQAMRHGLL PRTLHVDEPS EHVDWSSGAM SLLTEPRPWE AGDRPRRAGV
SSFGISGTNA HVVLEEAPAD ARVRGGERTD GSTPVPVPLS ARSAGGLRGQ AEKLAQHMAD
VPEADLADVA FSQAISRAGL ERRAVVLASD RGELLEGLAA LRDGEPGGDL VRGVAGGGGL
ALLFTGQGSQ RIGMGEELHA RFPVYAKAFD EICAALDPHL ERPLREVVAG EPDPGLLDLT
AYTQPALFAV ETALYRLVES WGVRPDFVAG HSLGEIVAAH VSGVLSLEDA ARLVAGRGRL
IQALPAGGAM TAVQAGEEDV AELLAGRERQ VDVAAVNSPT SVVVSGDEDA VAAVAEECAS
RGWKTRRLRV AHAFHSPRMD AMLDDFGAVA ASLTYHRPRI AVLSNVSGKV LSAEELASPE
YWVQHVRRPV RFNDAVRTLW NQGVRTFVEL GPDAALTPMA AESLAELGQD AECVATMRRD
RPAVPTVLTA LSTMYARGAS VDWPEVFAGT GARRVDLPTY AFQHRRYWPE VSGTGEPEVR
ERTAVAVVEG AQDDEPSFRE RWHALAGPER FAALLELVRA EAATTLGNLE SGLATDDAFF
EVGFTSLNAV ELRNRLSGHT GLRLPTTLLF EYPTPQMLAE HLHERMAADQ EA
//
