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Database: UniProt
Entry: A4RSK1_OSTLU
LinkDB: A4RSK1_OSTLU
Original site: A4RSK1_OSTLU 
ID   A4RSK1_OSTLU            Unreviewed;       840 AA.
AC   A4RSK1;
DT   15-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   15-MAY-2007, sequence version 1.
DT   31-JUL-2019, entry version 89.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   Name=URE1 {ECO:0000313|EMBL:ABO94771.1};
GN   ORFNames=OSTLU_30221 {ECO:0000313|EMBL:ABO94771.1};
OS   Ostreococcus lucimarinus (strain CCE9901).
OC   Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC   Bathycoccaceae; Ostreococcus; unclassified Ostreococcus.
OX   NCBI_TaxID=436017 {ECO:0000313|EMBL:ABO94771.1, ECO:0000313|Proteomes:UP000001568};
RN   [1] {ECO:0000313|EMBL:ABO94771.1, ECO:0000313|Proteomes:UP000001568}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCE9901 {ECO:0000313|EMBL:ABO94771.1,
RC   ECO:0000313|Proteomes:UP000001568};
RX   PubMed=17460045; DOI=10.1073/pnas.0611046104;
RA   Palenik B., Grimwood J., Aerts A., Rouze P., Salamov A., Putnam N.,
RA   Dupont C., Jorgensen R., Derelle E., Rombauts S., Zhou K., Otillar R.,
RA   Merchant S.S., Podell S., Gaasterland T., Napoli C., Gendler K.,
RA   Manuell A., Tai V., Vallon O., Piganeau G., Jancek S., Heijde M.,
RA   Jabbari K., Bowler C., Lohr M., Robbens S., Werner G., Dubchak I.,
RA   Pazour G.J., Ren Q., Paulsen I., Delwiche C., Schmutz J., Rokhsar D.,
RA   Van de Peer Y., Moreau H., Grigoriev I.V.;
RT   "The tiny eukaryote Ostreococcus provides genomic insights into the
RT   paradox of plankton speciation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7705-7710(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
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DR   EMBL; CP000582; ABO94771.1; -; Genomic_DNA.
DR   RefSeq; XP_001416478.1; XM_001416441.1.
DR   STRING; 242159.ABO94771; -.
DR   EnsemblPlants; ABO94771; ABO94771; OSTLU_30221.
DR   GeneID; 5000412; -.
DR   Gramene; ABO94771; ABO94771; OSTLU_30221.
DR   KEGG; olu:OSTLU_30221; -.
DR   eggNOG; ENOG410IGM6; Eukaryota.
DR   eggNOG; ENOG410JX8F; Eukaryota.
DR   eggNOG; COG0804; LUCA.
DR   eggNOG; COG0831; LUCA.
DR   eggNOG; COG0832; LUCA.
DR   HOGENOM; HOG000075064; -.
DR   KO; K01427; -.
DR   OMA; GFDSHIH; -.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000001568; Chromosome 2.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000001568};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000001568}.
FT   DOMAIN      403    840       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   REGION      132    151       Disordered. {ECO:0000256|SAM:MobiDB-
FT                                lite}.
FT   ACT_SITE    594    594       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       408    408       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       410    410       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       491    491       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       491    491       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       520    520       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       546    546       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       634    634       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     493    493       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     491    491       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   840 AA;  90206 MW;  618DDD9C7BCCB6DE CRC64;
     MRLTPCEIDK LNILFAAGRV AQRRLARGMR LNHPEAVALI AMQCVELVRD PKFDKDGERR
     ALTSVEVQDL GKRVLGRRHV LDGVPELVGD VQIETTFDDG TKLITIHDAI CADDVDLELA
     LLGSFLPKPS EKAFPPATKE KTPAPGEVRT SKDSLELLAG RPKRKLKVTN TCDRPIQVGS
     HFHLIEANRF LTFDRRRAYG ARLAIPSGTA VRFEPGESKT VNMVNIAGNR VVKGGNNLVN
     GPATADRLDE VMKRVIDGGF GHVDADDLGE GEPLMIPRHK YAHMYGPTIG DRVRLGDTNL
     YITPERDLTM KGEESKFGGG KTLREGMSQQ AGVGDADSLD TIITNALIVD HSGIYKADIG
     IKDGHIVGIG HGGNPDVADV TPGMVVGVNT EAIAAEGCIV TAGALDTHIH YICPQLCTEA
     VASGITTLLG GGTGPASGTC ATTCTPSAAH MQFMLETTDA LPLNFAFTGK GNTASPEGLH
     EIIKAGAVGM KLHEDWGTTP AAIDNCLTIA EEYDVAVTIH TDTLNESCCV EKSIEAFKGR
     TIHTYHSEGA GGGHAPDIIK VCGEKMVLPS STNPTRPYTK NTVDEHLDML MVCHHLDPEI
     PEDVAFAESR IRAETIAAED VLHDMGALSI MASDSQAMGR VGEVIRRTWQ TAHSNKEQRG
     FLEEDANSGA DNVRVKRYVA KYTINPAIAH GMSHKVGSLE VGKFADVVIW KPAFFGAKPE
     IVVKGGQIAW AQMGDPNASI PTPEPVIMRG MFGALKPGKT CIAFVSAAAA AADVGAEYGL
     NKRVEAVVKC RGLSKDDMVL NNACPRIEVD PETYEVRADG VVLKSQPAQE LPLARRYFIV
//
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