ID LSRA_ENT38 Reviewed; 495 AA.
AC A4WER4;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=Autoinducer 2 import ATP-binding protein LsrA {ECO:0000250|UniProtKB:P77257};
DE Short=AI-2 import ATP-binding protein LsrA {ECO:0000250|UniProtKB:P77257};
DE EC=7.6.2.13 {ECO:0000250|UniProtKB:P77257};
GN Name=lsrA; OrderedLocusNames=Ent638_3536;
OS Enterobacter sp. (strain 638).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Enterobacter.
OX NCBI_TaxID=399742;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=638;
RX PubMed=20485560; DOI=10.1371/journal.pgen.1000943;
RA Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D.,
RA Vangronsveld J., Newman L., Monchy S.;
RT "Genome sequence of the plant growth promoting endophytic bacterium
RT Enterobacter sp. 638.";
RL PLoS Genet. 6:E1000943-E1000943(2010).
CC -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC autoinducer 2 (AI-2) import. Responsible for energy coupling to the
CC transport system. {ECO:0000250|UniProtKB:P77257}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + (2R,4S)-2-methyl-2,3,3,4-
CC tetrahydroxytetrahydrofuran-[AI-2-binding protein]Side 1 = ADP +
CC phosphate + (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuranSide
CC 2 + [AI-2-binding protein]Side 1.; EC=7.6.2.13;
CC Evidence={ECO:0000250|UniProtKB:P77257};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC (LsrB). {ECO:0000250|UniProtKB:P77257}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P77257}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P77257}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. AI-2
CC autoinducer porter (TC 3.A.1.2.8) family. {ECO:0000305}.
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DR EMBL; CP000653; ABP62194.1; -; Genomic_DNA.
DR RefSeq; WP_015960520.1; NC_009436.1.
DR AlphaFoldDB; A4WER4; -.
DR SMR; A4WER4; -.
DR STRING; 399742.Ent638_3536; -.
DR KEGG; ent:Ent638_3536; -.
DR eggNOG; COG1129; Bacteria.
DR HOGENOM; CLU_000604_92_3_6; -.
DR OrthoDB; 9805029at2; -.
DR Proteomes; UP000000230; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd03216; ABC_Carb_Monos_I; 1.
DR CDD; cd03215; ABC_Carb_Monos_II; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43790:SF2; AUTOINDUCER 2 IMPORT ATP-BINDING PROTEIN LSRA; 1.
DR PANTHER; PTHR43790; CARBOHYDRATE TRANSPORT ATP-BINDING PROTEIN MG119-RELATED; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Repeat; Translocase; Transport.
FT CHAIN 1..495
FT /note="Autoinducer 2 import ATP-binding protein LsrA"
FT /id="PRO_0000351289"
FT DOMAIN 5..233
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 256..494
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 37..44
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 495 AA; 54392 MW; D8BC625426C96FE2 CRC64;
MTVLIEAHGI HKQFSGVPVL RGIDFTLLSG QVHALMGGNG AGKSTLMKII AGVETPDSGE
LNVEGKPFAR LKPGQAHQLG IYLVPQEPML FPNLTVRENI LFRLPRGNDP EKRLADKLQQ
LQCQLNLDAA ASTLEVADQQ MVEILRGLMR DAKILILDEP TASLTPGETE RLFRQIRALQ
DLGVGIVFIS HKLPEIRQLA SHVSVMRDGA VVLSGETTQF DDTALITAMT PVSREQGLSD
TQKLWLALPG NRRTQAQDFP VLRVEDLTGE GFIDLSLEIY AGEIVGLAGL VGSGRTEFAE
TLYGLRPTRG GRIWLENQEI GEDSVLTRLE KGLVYLPEDR QVSGLFLDAP IRWNTVALNE
PSLWQQRKRE AAVVERYHRA LGIKLNHPDQ IVRTLSGGNQ QKVLLARCLE ANPLLLIVDE
PTRGVDVSAR ADIYQLIKSV AAQNVAVLMI SSDLDEFPGL ADRVLVMHQG MFSGELPRHA
VSLDRMMALA FGGQS
//
