ID A4YQE9_BRASO Unreviewed; 1058 AA.
AC A4YQE9;
DT 29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2007, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE SubName: Full=Putative chemotaxis protein methyltransferase {ECO:0000313|EMBL:CAL76125.1};
GN OrderedLocusNames=BRADO2290 {ECO:0000313|EMBL:CAL76125.1};
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=114615 {ECO:0000313|EMBL:CAL76125.1, ECO:0000313|Proteomes:UP000001994};
RN [1] {ECO:0000313|EMBL:CAL76125.1, ECO:0000313|Proteomes:UP000001994}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278 {ECO:0000313|Proteomes:UP000001994};
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000256|ARBA:ARBA00000085};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-
CC glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:24452, Rhea:RHEA-COMP:10208, Rhea:RHEA-COMP:10311,
CC ChEBI:CHEBI:29973, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:82795; EC=2.1.1.80;
CC Evidence={ECO:0000256|ARBA:ARBA00001541};
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DR EMBL; CU234118; CAL76125.1; -; Genomic_DNA.
DR AlphaFoldDB; A4YQE9; -.
DR STRING; 114615.BRADO2290; -.
DR KEGG; bra:BRADO2290; -.
DR eggNOG; COG1352; Bacteria.
DR eggNOG; COG1579; Bacteria.
DR eggNOG; COG2201; Bacteria.
DR eggNOG; COG3920; Bacteria.
DR HOGENOM; CLU_000892_0_1_5; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0004673; F:protein histidine kinase activity; IEA:InterPro.
DR GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:InterPro.
DR GO; GO:0008983; F:protein-glutamate O-methyltransferase activity; IEA:RHEA.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.155.10; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR Gene3D; 1.10.287.620; Helix Hairpins; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR035909; CheB_C.
DR InterPro; IPR022642; CheR_C.
DR InterPro; IPR000780; CheR_MeTrfase.
DR InterPro; IPR022641; CheR_N.
DR InterPro; IPR036804; CheR_N_sf.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011102; Sig_transdc_His_kinase_HWE.
DR InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR PANTHER; PTHR24422; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR PANTHER; PTHR24422:SF25; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR Pfam; PF01339; CheB_methylest; 1.
DR Pfam; PF01739; CheR; 1.
DR Pfam; PF03705; CheR_N; 1.
DR Pfam; PF07536; HWE_HK; 1.
DR Pfam; PF13596; PAS_10; 1.
DR PRINTS; PR00996; CHERMTFRASE.
DR SMART; SM00911; HWE_HK; 1.
DR SMART; SM00138; MeTrc; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF47757; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50122; CHEB; 1.
DR PROSITE; PS50123; CHER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Chemotaxis {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:CAL76125.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001994};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CAL76125.1}.
FT DOMAIN 22..174
FT /note="CheB-type methylesterase"
FT /evidence="ECO:0000259|PROSITE:PS50122"
FT DOMAIN 213..483
FT /note="CheR-type methyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50123"
FT COILED 654..723
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 28
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 56
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 148
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
SQ SEQUENCE 1058 AA; 118082 MW; 431A4566469ED3DF CRC64;
MQRDMATGGV SQSGDKKRRP LVIGVGASTS HTESLARFLS RAALDQDLAI VLVLRHREGL
DEEWLRQVLQ PLENVRLRAL EDGAEIEGQV IYLCPPDMIA TLHDNRAVLR QSPQAPGERA
TIDSLLVSLA EERAEQAIGV ILAGTGGDGT LGVAALKDHG GLALAERPVG EEEEPQDSSR
AAAIADFVLA AEDIPDHIQV YARHLRRLED RQGFDEVLAA AATSLDRIAD ILRTKTGNDF
HGYKQNTFLR RVQRRMQVAQ IGDVSAYVDF LRNDKDEVQH LFNDLLIGVT EFFRDRREFE
VLEQDVIPQI FAGRQRGHPI RVWVLGCATG EEAYSIAILL REHAARLDAP PPLQIFATDI
DGKALAAARV GRYRTDIEAN ISPERLARWF VREGDTYCVV KELREMCIFS QHNVIKDAPF
SKLDLVSCRN LLIYLNAELQ NRVIPLFHFS LLPGRFLFLG NSENVTRHPK LFAPVDRRAR
IFRKLDGGSR LPPEFPISTA AGRATVDMPA IRPFNADVGL ERKAQRIAER YAPAYVITDS
NFQILHFSGR TGRFIEPTAG VATLDLLGLV HRDLRLELRT ALGHARDFNQ IVHADQVMLG
VNGHTIGVDI TVEPVEDSDN GHRNFVVLFK DGPLRPPATA LASGSSALVR SEHVERLESE
LRATRERLQA TIEELESTNE ELKSSNEEYQ SLNEELQSAN EELETSREEL QSVNEELTTV
NGELAHRVQE LTRATSDLKN FLESTQIATV FVDNDLRVMN FTPAITQVLH LVETDVGRPI
AHIKARIPIK ELYEDIRRVL RTLASAEREL IAPDSGTRFI VRMLPYRSVD NFIAGVVMTF
IDVTAITRAE ERQRLLLAEL QHRVRNTLGV VRSIARRSAQ TSSTVEEYAA HLDGRLGALA
RTQAAVTRDP QAGVDLEYLL ADELMAYKAR EGEQVRISGP SMRLQPKAAE TLSLAIHELA
TNAVKYGALS QPTGRIDISW HIDESVTPKE LVFEWRERGG PEVKPSKRKG FGSEILERTL
AFELKGRTQL SFNPAGMQFQ IILPLSRSFV QGPATGQR
//
