ID A4Z171_BRASO Unreviewed; 402 AA.
AC A4Z171;
DT 29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2007, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN OrderedLocusNames=BRADO6256 {ECO:0000313|EMBL:CAL79897.1};
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=114615 {ECO:0000313|EMBL:CAL79897.1, ECO:0000313|Proteomes:UP000001994};
RN [1] {ECO:0000313|EMBL:CAL79897.1, ECO:0000313|Proteomes:UP000001994}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278 {ECO:0000313|Proteomes:UP000001994};
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000256|ARBA:ARBA00002510}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC {ECO:0000256|RuleBase:RU362101}.
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DR EMBL; CU234118; CAL79897.1; -; Genomic_DNA.
DR RefSeq; WP_012029783.1; NC_009445.1.
DR AlphaFoldDB; A4Z171; -.
DR STRING; 114615.BRADO6256; -.
DR KEGG; bra:BRADO6256; -.
DR eggNOG; COG2215; Bacteria.
DR HOGENOM; CLU_058605_0_2_5; -.
DR OrthoDB; 9812956at2; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR Pfam; PF03824; NicO; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW Reference proteome {ECO:0000313|Proteomes:UP000001994};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362101};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362101};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..402
FT /note="Nickel/cobalt efflux system"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002676862"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 118..143
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 155..174
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 325..352
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 373..396
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT REGION 201..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..241
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..275
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 402 AA; 41463 MW; B1BC4B02304B8704 CRC64;
MTPRPSSVRP FLICAAILAA LLSADAASHV LLAKSPFGAP AGEPQVGGIV GWLLAKQSEF
YRQMSGAIRS AKEDGSAVWW LLAISFAYGI FHAAGPGHGK AVIASYLVAN QETAKRGIVL
SFASGLMQAL VAVAIVGIGA VLLNATAATM CSAEKVVEIA SYGLIALLGA RLVWVKGAAF
LRTWQPATPA LALAGGPAAA VVSSGHDHGH AHDHQHRAPQ DHHGHDHHHG HDHHGHDHHH
GHAHTQAAAA HGHAAHDHVH HDHVHHDHVH GDDCGCGHSH GPAPSELAGP GGWQRGFQAI
FAAGIRPCSG AILVLVFALA QGMFWAGVAA TIVMGMGTAI TVATIAVIAV AAKDIARRLS
AGRDGGGALI MRGLEFGAAG LVLLFGVGLL FGYMAAERTT CF
//
