GenomeNet

Database: UniProt
Entry: A5F3R4
LinkDB: A5F3R4
Original site: A5F3R4 
ID   RHLB_VIBC3              Reviewed;         438 AA.
AC   A5F3R4; C3M3W6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   11-DEC-2019, entry version 76.
DE   RecName: Full=ATP-dependent RNA helicase RhlB {ECO:0000255|HAMAP-Rule:MF_00661};
DE            EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_00661};
GN   Name=rhlB {ECO:0000255|HAMAP-Rule:MF_00661};
GN   OrderedLocusNames=VC0395_A2697, VC395_0349;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: DEAD-box RNA helicase involved in RNA degradation. Has RNA-
CC       dependent ATPase activity and unwinds double-stranded RNA.
CC       {ECO:0000255|HAMAP-Rule:MF_00661}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00661};
CC   -!- SUBUNIT: Component of the RNA degradosome, which is a multiprotein
CC       complex involved in RNA processing and mRNA degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_00661}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00661}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. RhlB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00661}.
DR   EMBL; CP000627; ABQ21168.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08374.1; -; Genomic_DNA.
DR   RefSeq; WP_000750771.1; NC_012582.1.
DR   SMR; A5F3R4; -.
DR   STRING; 345073.VC395_0349; -.
DR   PRIDE; A5F3R4; -.
DR   EnsemblBacteria; ABQ21168; ABQ21168; VC0395_A2697.
DR   EnsemblBacteria; ACP08374; ACP08374; VC395_0349.
DR   KEGG; vco:VC0395_A2697; -.
DR   KEGG; vcr:VC395_0349; -.
DR   PATRIC; fig|345073.21.peg.336; -.
DR   eggNOG; ENOG4105C1J; Bacteria.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268807; -.
DR   KO; K03732; -.
DR   OMA; TRFHDFK; -.
DR   BioCyc; VCHO345073:G1GU4-361-MONOMER; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00661; DEAD_helicase_RhlB; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR023554; RNA_helicase_ATP-dep_RhlB.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding;
KW   RNA-binding.
FT   CHAIN           1..438
FT                   /note="ATP-dependent RNA helicase RhlB"
FT                   /id="PRO_1000082876"
FT   DOMAIN          40..219
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00661"
FT   DOMAIN          245..390
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00661"
FT   NP_BIND         53..60
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00661"
FT   MOTIF           9..37
FT                   /note="Q motif"
FT   MOTIF           165..168
FT                   /note="DEAD box"
SQ   SEQUENCE   438 AA;  49072 MW;  CB786E75162BC125 CRC64;
     MKKTHITEHK FADFGLQPQV IDGLEKKGFV YCTPIQALAL PVLLSGQDIA GQAQTGTGKT
     LAFLTATFNH LLTTPAAEGR AETQPRAIIM APTRELAIQI FNDAEPLLAS TGLKAALAYG
     GESYDKQLAK LQSGVDILIG TTGRIIDFYK QRVFNLNHIQ AVVLDEADRM FDLGFIKDIR
     FLFRRMPEPK DRLNMLFSAT LSYRVQELAF EHMNNPEHVV VEPEQKTGHR IQEELFYPSN
     EHKMALLQTL IEEEWPDRAI IFANTKHRCE QIWAHLAADN HRVGLLTGDV PQKKRERILE
     QFTQGDVDIL VATDVAARGL HIPQVTHVFN YDLPDDCEDY VHRIGRTGRA GASGHSISFA
     CEEYAINLPA IESYIEHAIP TSDYDPSALL TDLPAPLSLR SSPQQRRTNT AGSRNSNNGG
     NRKPQQRRPR APRPKKEA
//
DBGET integrated database retrieval system