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Database: UniProt
Entry: A5F5Z2
LinkDB: A5F5Z2
Original site: A5F5Z2 
ID   RIMK_VIBC3              Reviewed;         301 AA.
AC   A5F5Z2; C3M3N8;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   16-JAN-2019, entry version 67.
DE   RecName: Full=Probable alpha-L-glutamate ligase {ECO:0000255|HAMAP-Rule:MF_01552};
DE            EC=6.3.2.- {ECO:0000255|HAMAP-Rule:MF_01552};
GN   Name=rimK {ECO:0000255|HAMAP-Rule:MF_01552};
GN   OrderedLocusNames=VC0395_A1871, VC395_2397;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y.,
RA   Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the
RT   cholera pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01552};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01552};
CC       Note=Binds 2 magnesium or manganese ions per subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_01552};
CC   -!- SIMILARITY: Belongs to the RimK family. {ECO:0000255|HAMAP-
CC       Rule:MF_01552}.
DR   EMBL; CP000627; ABQ22059.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP10387.1; -; Genomic_DNA.
DR   RefSeq; WP_000689982.1; NC_012582.1.
DR   ProteinModelPortal; A5F5Z2; -.
DR   SMR; A5F5Z2; -.
DR   STRING; 345073.VC0395_A1871; -.
DR   EnsemblBacteria; ABQ22059; ABQ22059; VC0395_A1871.
DR   EnsemblBacteria; ACP10387; ACP10387; VC395_2397.
DR   KEGG; vco:VC0395_A1871; -.
DR   KEGG; vcr:VC395_2397; -.
DR   PATRIC; fig|345073.21.peg.2311; -.
DR   eggNOG; ENOG4105D9I; Bacteria.
DR   eggNOG; COG0189; LUCA.
DR   HOGENOM; HOG000293092; -.
DR   KO; K05844; -.
DR   OMA; YEHKVFY; -.
DR   BioCyc; VCHO345073:G1GU4-2419-MONOMER; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006464; P:cellular protein modification process; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   HAMAP; MF_01552; RimK; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013651; ATP-grasp_RimK-type.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR023533; RimK.
DR   InterPro; IPR004666; RpS6_RimK/Lys_biosynth_LsyX.
DR   Pfam; PF08443; RimK; 1.
DR   TIGRFAMs; TIGR00768; rimK_fam; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Protein biosynthesis.
FT   CHAIN         1    301       Probable alpha-L-glutamate ligase.
FT                                /FTId=PRO_1000073566.
FT   DOMAIN      104    287       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_01552}.
FT   NP_BIND     178    179       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   NP_BIND     211    213       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       248    248       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       260    260       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       260    260       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       262    262       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   BINDING     141    141       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   BINDING     187    187       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
SQ   SEQUENCE   301 AA;  32442 MW;  A33E4C1FBCCA7BED CRC64;
     MKIGILSRNA SLYSTKRLIE ACKQRGHEVR VIDALRCYMN INSDKPEIHY KGEELAGFDA
     VIPRIGASVT FYGTAVLRQF EMMGVYPANE SVAITRSRDK LRSMQLLSRR GIGMPITGFA
     SKPDDVKDLL DMVGGAPVVI KLLEGTQGIG VVLAETRTAA ESVIEAFMGL KANIMVQEYI
     KEAGGADIRC FVIGDKVIAA MKRQGADGEF RSNLHRGGTA SLVKITPQER KTAIEAAKIM
     GLNVAGVDLL RSARGPLVME VNSSPGLEGI EAATGKDIAG MIVEFIEKNA ASKRTKTRGK
     G
//
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