ID A5N066_CLOK5 Unreviewed; 547 AA.
AC A5N066;
DT 10-JUL-2007, integrated into UniProtKB/TrEMBL.
DT 10-JUL-2007, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=PABS domain-containing protein {ECO:0000259|PROSITE:PS51006};
GN OrderedLocusNames=CKL_2500 {ECO:0000313|EMBL:EDK34512.1};
OS Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=431943 {ECO:0000313|EMBL:EDK34512.1, ECO:0000313|Proteomes:UP000002411};
RN [1] {ECO:0000313|EMBL:EDK34512.1, ECO:0000313|Proteomes:UP000002411}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680
RC {ECO:0000313|Proteomes:UP000002411};
RX PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA Hagemeier C., Thauer R.K., Gottschalk G.;
RT "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT metabolic features.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}.
CC -!- SIMILARITY: Belongs to the spermidine/spermine synthase family.
CC {ECO:0000256|ARBA:ARBA00007867}.
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DR EMBL; CP000673; EDK34512.1; -; Genomic_DNA.
DR RefSeq; WP_012102846.1; NC_009706.1.
DR AlphaFoldDB; A5N066; -.
DR STRING; 431943.CKL_2500; -.
DR KEGG; ckl:CKL_2500; -.
DR eggNOG; COG4262; Bacteria.
DR HOGENOM; CLU_008530_1_0_9; -.
DR Proteomes; UP000002411; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006596; P:polyamine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR030374; PABS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; NF037959; MFS_SpdSyn; 1.
DR PANTHER; PTHR43317:SF9; POLYAMINE AMINOPROPYLTRANSFERASE 2; 1.
DR PANTHER; PTHR43317; THERMOSPERMINE SYNTHASE ACAULIS5; 1.
DR Pfam; PF01564; Spermine_synth; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51006; PABS_2; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Polyamine biosynthesis {ECO:0000256|ARBA:ARBA00023115, ECO:0000256|PROSITE-
KW ProRule:PRU00354}; Reference proteome {ECO:0000313|Proteomes:UP000002411};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00354}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 84..108
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 155..179
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 185..206
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 213..235
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 216..461
FT /note="PABS"
FT /evidence="ECO:0000259|PROSITE:PS51006"
FT ACT_SITE 374
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00354"
SQ SEQUENCE 547 AA; 61190 MW; 706D13E99E82C956 CRC64;
METRTILKNK FYLYMTEFFA GMSVMAVELA ASRLLAPYFS SSQIVWTIII GTIMIAIALG
NIWGGRSADK NPNPDKLYMR MIIAAIWIAA IPLLGKYIIL AVSAALVLTI SNNFLILATF
LVCMIIFVFP LFLLGTVTPS LVKYTVSSLS DNGKIVGMLG AINTIGSIIG TFLPTFVTIP
AVGTSVTFLI FSGILLMLGI IYFISIKAKK VQCIISVILF IFCSVFGSSD SFAFWEKNLL
YEGESIYNYL QVKDTNDSII LSTNVLFGVQ SIMKKDNSLT GMYYDYALAA PVMSGIHKKK
NAKILVLGMG TGTYAKQCEK YFPSASIEGV EIDQKITNLA SKYFKLPNTI KVNTYDGRAY
LNSENKKYDV IMVDAYQDIT IPFQMSSKEF FTIVKNHLVK DGVMVVNMNM HTEKKGNITQ
HLSDTIANVF NKVYTVNVDG STNRELFATQ NMEAAKTFEQ NTRELRNNEL KTLMYNIEDN
MDTYVKGNYL LTDDKAPVEL LGMRVIDELI QDELSYYKKI FKEKYKDLII QGGEIDRAEA
GGLDFPN
//
