ID A6RBU7_AJECN Unreviewed; 2591 AA.
AC A6RBU7;
DT 21-AUG-2007, integrated into UniProtKB/TrEMBL.
DT 21-AUG-2007, sequence version 1.
DT 27-MAR-2024, entry version 76.
DE RecName: Full=alpha-1,3-glucan synthase {ECO:0000256|ARBA:ARBA00012688};
DE EC=2.4.1.183 {ECO:0000256|ARBA:ARBA00012688};
GN ORFNames=HCAG_07105 {ECO:0000313|EMBL:EDN10644.1};
OS Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus)
OS (Histoplasma capsulatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma.
OX NCBI_TaxID=2059318 {ECO:0000313|EMBL:EDN10644.1, ECO:0000313|Proteomes:UP000009297};
RN [1] {ECO:0000313|Proteomes:UP000009297}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NAm1 / WU24 {ECO:0000313|Proteomes:UP000009297};
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC alpha-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:19749, Rhea:RHEA-
CC COMP:11150, Rhea:RHEA-COMP:11151, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28100, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.183; Evidence={ECO:0000256|ARBA:ARBA00000687};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR602640-2};
CC Note=Binds 2 calcium ions per subunit. {ECO:0000256|PIRSR:PIRSR602640-
CC 2};
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|ARBA:ARBA00006122}.
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DR EMBL; CH476662; EDN10644.1; -; Genomic_DNA.
DR STRING; 339724.A6RBU7; -.
DR VEuPathDB; FungiDB:HCAG_07105; -.
DR HOGENOM; CLU_000488_0_0_1; -.
DR OMA; NIGPGPI; -.
DR OrthoDB; 141134at2759; -.
DR Proteomes; UP000009297; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0047657; F:alpha-1,3-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0004064; F:arylesterase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11323; AmyAc_AGS; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.120.10.30; TolB, C-terminal domain; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR002640; Arylesterase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR PANTHER; PTHR47182; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED; 1.
DR PANTHER; PTHR47182:SF2; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK13; 1.
DR Pfam; PF01731; Arylesterase; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF63829; Calcium-dependent phosphotriesterase; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR602640-2};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR602640-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000009297};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..2591
FT /note="alpha-1,3-glucan synthase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002699960"
FT TRANSMEM 1896..1913
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1920..1943
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1955..1975
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1987..2007
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2096..2116
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2123..2145
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2165..2187
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2224..2243
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 65..488
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT BINDING 2334
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR602640-2"
FT BINDING 2398
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR602640-2"
FT BINDING 2445
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR602640-2"
FT BINDING 2446
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR602640-2"
SQ SEQUENCE 2591 AA; 292788 MW; 3035789924EA9AAD CRC64;
MRRWLVGVLL ALLAARATSW PYDPQFEGYN LNENKSAISP ADYYGEWSGH QYHPSPDNWR
FPFYSLFLDR FVNGDPTNDN INGTSYEHDL NSNQMRHGGD VAGLMDTLDY LQGMGIKHFG
TIESFRMTIT EIHRRGMYVI MDNTLATLGD LIGFKGHLNG TTPFDTNEHQ VVWKSSRRYA
DFRIGNEYNE TCDYPRFYWE NGYPVGTDVT DQLIGCYDSD FDQYGDIEAF GVYPDWQRQL
SKFASVQDRL REWIPSVRER LMRHSCILMR QLDIDGFRYD KALQATVDAL GDMSEHYREC
ARSLGKHNFF LPGEITGGNT LGAIYIGRGR QPNMLPDSLE AAVKMTHRSN ENYFIREQGK
SALDAGAFHY SVYRSLTRFL GMDGNLAAGF DTPVNWVDAW NEMILTNDFV NSQGHFDPRH
MFGATNQDVF RWPTIREGTQ RMLLAFYITT LHLPGIPLLL WGEEQAFYVL DNTAQNYIFG
RQAMSPATAW QTHGCYKLGS QQYYQWPIES GKYGCHDETV SYDHRDPSHP VRNILKTMYQ
MRENYRTLND GFFIQQLSNQ TWQIFLPGSN NTPTETGMWS VHRGRFETAQ DFGKESNLPV
WLVYQNYNKT HKYEFDCSDE KKALISNFDS GTTVKNLFFP FDELTLKRGP KKLGIEQSRE
FNGCLDSLEL KPWDFRAYVP RERFIRPRPM VTKFSPGHDA RLLSTVSPDK DERVPIELYY
SEAMDCNSVT ASITLNSTTD NGKIPSIDYS TVQCQSLNPQ PTKYIGELPN FWRWSATLVG
VYNGVHQLSI KDALSADRRT NTSSMDHFLF RIGQSDNPMV FTRAANYSSS FLHTDKRGSL
YVSHHAAGAD LYRYSLNWGS SFSDWLPYTG GNTTLERQPW SGTKLQRWKG EHVIVEYWNR
MTGSSSHVQH GDLDWPHNKP RRFPHLYWNG PYNQYGYDAG LRNHMVQDDK GIWRFRFMTE
WPALAQVNVW GINPDGKPDQ TFVFGDIDGD SVLDRLPPSS LSNIVINITN PPPHPYLSWL
FLLNDGTLHF ELYPIGNQYH QLALYILLWV IPILTAAIGI YVFKKSFYQV KFNQVGISDK
RGFIPLAIRR KFNKRVLEES HSMNPLVKLA NKSRFLQSTS ALVADSGSRR TTLIATLEYH
IQDWGIKIKI GGLGVMAQLM GEHLGHQNLI WVIPCVSGID YPIDQVAEPM TVTVLGNPYE
VKVQYHVVRN ITYVLLDAPV FRQQTSAEPY PARMDDLDSA IYYSAWNQCI AFAIKRFPVD
LYHINDYHGS LAPIYLLPQT IPICLSLHNA EFQGLVHPLC QFGSIQLLHA GASYLRIHQQ
GFGAVGVSKK YGKRSYARYP IFWGLKKVGT LPNPDPSDTG EWNGELPNKS DIDVNPQFEA
DRLELKRQAQ EWAGLDQNPN ADLLVFVGRW SMQKGIDLIA DVFPAILEAR KDVQLLCVGP
IIDLYGKFAA LKLDKLMKLY PGRVFSRPQF TALPPFIFSG AEFALIPSRD EPFGLVAVEF
GRKGALGIGA RVGGLGQMPG WWYTVESTTT SHLLNQFKLA IDSALNSKYE TRAMMRARSA
KQRFPVAQWV EDLEILQSTA IRIHNKESAK STGQPHYTPS AYSSSGILGS PIGLGSMTPV
SPMSQSRNQS CINTNRLSAF GPQARNTVVY HQEVCPGADI DNENQPTGLS RKLSLGVRSG
PGHTRAAPRM PRKLRKILAP MDLPENVHEN EPDRLGVAVT TDVDDDSDDD TVAQYFGDDE
YTLTREQAEA GRGNDMSPFH PGFNELRIPG ESRPPSPAGM EGFLSPSRAL ADSANCFGST
SVLSLGSVVG GKRDFKLQKV DPFFTDSNGE FYRAFEKKLE DLNSHNSESD LCIEDYLVKS
EKKWFNRFRD ARLRPPPRQS RSHCSLVKLA QSATKLYAVA TTYLITSIIW WFIFRWFKSV
IPLSLPFFFY GLAFMFIGVA HFAPTDDGVG WIQNIGAALY AAASSSGSIF FALNFGDESG
APVKDWVFRA CVIQGTQQIY VVALWYWGSK LTKNIALGII DPDPISSTWK LTIFFSAHLT
VETGPYPPTA TDVWLIIAIY LGAPRWAQIW WGTSGIGQHV PWAGGYLPGA LISRSLWLWL
GVLDSMQGVG FGMILLQTLT RFHICFTLLA AQFLGSIATM CARAFSPNRI GPGDVHPDIS
GGIDTIWNAW FWICLFCQLA LCSLLFYPQL TLFVPRKDLS QVTSTSVVQA VMAISVIGKF
NYQIAATFAI IIGTISTVFY KPLVTKIHTL HNLPGEWHPF DGGSADIRFS DTIKFSEDIV
IDNDRGIAII SFDPGRSGWN TVMVNTVFDI DYDLYQAKYI TTVSDNNETI VSPNSIAPVS
YTQFYVTNDH HYLARTRPLL NKIETYFSMP WGWVTFVDFS STEHLKCEIV ATNISFANGI
VMTPTGKEVV VASSGHDAVY IYQRDSETNS LSSTHETVHV NFHPDNVNFD HSLVISDPTV
FDADGRFLRG LTVGGHPSII KLVQAVHNPE SVKAPSWVAE IRRGAGVDPA PCPAAPQQPK
FYARTLYQSN GSAFFDQRYR GSRFQTRPFT DCFFIRERWK RLSRGDSALA RGLFKWNWSD
LDSKATADHV F
//
