GenomeNet

Database: UniProt
Entry: A6RW79
LinkDB: A6RW79
Original site: A6RW79 
ID   PRP5_BOTFB              Reviewed;        1151 AA.
AC   A6RW79;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   16-OCT-2019, entry version 67.
DE   RecName: Full=Pre-mRNA-processing ATP-dependent RNA helicase prp5;
DE            EC=3.6.4.13;
GN   Name=prp5; ORFNames=BC1G_04866;
OS   Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis
OS   cinerea).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Sclerotiniaceae; Botrytis.
OX   NCBI_TaxID=332648;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B05.10;
RX   PubMed=21876677; DOI=10.1371/journal.pgen.1002230;
RA   Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P.,
RA   Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S.,
RA   Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M.,
RA   Pradier J.-M., Quevillon E., Sharon A., Simon A., ten Have A.,
RA   Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V.,
RA   Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z.,
RA   Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C.,
RA   Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S.,
RA   Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M.,
RA   Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C.,
RA   Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H.,
RA   Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C.,
RA   Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA   Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.;
RT   "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT   sclerotiorum and Botrytis cinerea.";
RL   PLoS Genet. 7:E1002230-E1002230(2011).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved spliceosome assembly
CC       and in nuclear splicing. Catalyzes an ATP-dependent conformational
CC       change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2
CC       snRNP association with intron RNA (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX46/PRP5
CC       subfamily. {ECO:0000305}.
DR   EMBL; CH476861; EDN23597.1; -; Genomic_DNA.
DR   RefSeq; XP_001556848.1; XM_001556798.1.
DR   SMR; A6RW79; -.
DR   PRIDE; A6RW79; -.
DR   EuPathDB; FungiDB:Bcin11g03310; -.
DR   OMA; SKFHSIR; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW   Nucleotide-binding; Nucleus.
FT   CHAIN         1   1151       Pre-mRNA-processing ATP-dependent RNA
FT                                helicase prp5.
FT                                /FTId=PRO_0000310226.
FT   DOMAIN      585    763       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      774    938       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     598    605       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       554    582       Q motif.
FT   MOTIF       711    714       DEAD box.
FT   COMPBIAS      3    142       Arg-rich.
SQ   SEQUENCE   1151 AA;  127330 MW;  EB7CBF3EC2E6F1FE CRC64;
     MARHRDSRSP SPTGSHHSSR RNRRDDNGRR DRDRRDDVRG QRRSRSPDRR ERDRDMNRRR
     DRSVGRLNDD YHRSGRRERS RDRRSYADRE RSPDRRRRRS RERDYRDRRD DSYDDRARRR
     REDSTDSWSR SRGDETRGQT PRSDAGAKVN DAPKVSTPAV QTEEQKKAER LAKLEAWKKK
     MAEDKERKEK ELAAGGTRKL LDAIDQKANG SPSLTSPNSP TTPATPATPG DLAPPTNYAG
     KFDPKAIAKK AVASSSSAHT LGKDLPLKEL SKASATLTST VKGLQADKKP TAFSSTSKVS
     ALPKTRGNLS VFGLGAKVTD NEKTSQKRAL DFDEDEGSRK KLEKLPSLPM ANTEEDDAAL
     ANGVEEQDDD DDADLEAAGT EEEAAAAARA AAEKREERLQ EAEAQPHTNG DVQMEDAPQP
     DSTAMDEDEE IDPLDAFMEE MGDPFSLPKN NATFIKDNIK SQPQEPEPLF GDDDVDLKAL
     DADPDEILAI ANKARKKKDI PTINYSALDL PPFRKNFYTE PTELAEMTEA EIADLRLELD
     GIKVAGKDVP KPVQKWSQCG LDVKSLDVIT KLGYERPTSI QMQAIPAIMS GRDVIGVAKT
     GSGKTIAFLL PMFRHIRDQR PLKGSDGPIG LIMTPTRELA TQIHKECKPF LKAMGLRAVC
     AYGGAIIKDQ IADLKRGAEI IVCTPGRMIE LLAANSGRVT NLQRVTYVVL DEADRMFDMG
     FEPQVMKVFN NIRPNRQTIL FSATMPRIMD ALAKKTLQSP VEIVVGGRSV VAPEITQIVE
     VREEKEKFHR LLELLGELYN TDEDARTLIF VDRQEKADDL LKDLMRKGYP CMSIHGGKDQ
     VDRDSTIDDF KAGVVPIMIA TSVAARGLDV KQLKLVVNFD APNHLEDYVH RAGRTGRAGN
     TGTAVTFITE EQEQYSVGIA KALEQSGQEV PDRLNEMRKS YKDKVKSGAK KESSGFRGKG
     LERFDAEREA TKARELVQPA ASTAPPKLLG VPKGIDLDGD IKVHRTEPAA TTGSKLDKVT
     SAIDAINARL NKTGQLRSGV PIDNKGPDAG AFHATLEIND FPQKARWAVT NRTNVAKILE
     ATGTSITTKG SFYPAGKEVQ ADPKLYILVE GDTEVVVTNA MRELMRLLKE GTMAAADAEG
     RAPVGGRYTV T
//
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