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Database: UniProt
Entry: A7ENE0
LinkDB: A7ENE0
Original site: A7ENE0 
ID   PRP5_SCLS1              Reviewed;        1114 AA.
AC   A7ENE0;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   16-OCT-2019, entry version 62.
DE   RecName: Full=Pre-mRNA-processing ATP-dependent RNA helicase prp5;
DE            EC=3.6.4.13;
GN   Name=prp5; ORFNames=SS1G_06839;
OS   Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White
OS   mold) (Whetzelinia sclerotiorum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Sclerotiniaceae; Sclerotinia.
OX   NCBI_TaxID=665079;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18683 / 1980 / Ss-1;
RX   PubMed=21876677; DOI=10.1371/journal.pgen.1002230;
RA   Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P.,
RA   Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S.,
RA   Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M.,
RA   Pradier J.-M., Quevillon E., Sharon A., Simon A., ten Have A.,
RA   Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V.,
RA   Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z.,
RA   Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C.,
RA   Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S.,
RA   Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M.,
RA   Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C.,
RA   Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H.,
RA   Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C.,
RA   Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA   Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.;
RT   "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT   sclerotiorum and Botrytis cinerea.";
RL   PLoS Genet. 7:E1002230-E1002230(2011).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved spliceosome assembly
CC       and in nuclear splicing. Catalyzes an ATP-dependent conformational
CC       change of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2
CC       snRNP association with intron RNA (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX46/PRP5
CC       subfamily. {ECO:0000305}.
DR   EMBL; CH476628; EDO04356.1; -; Genomic_DNA.
DR   RefSeq; XP_001592598.1; XM_001592548.1.
DR   SMR; A7ENE0; -.
DR   PRIDE; A7ENE0; -.
DR   GeneID; 5488511; -.
DR   KEGG; ssl:SS1G_06839; -.
DR   EuPathDB; FungiDB:SS1G_06839; -.
DR   InParanoid; A7ENE0; -.
DR   KO; K12811; -.
DR   OMA; SKFHSIR; -.
DR   Proteomes; UP000001312; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN         1   1114       Pre-mRNA-processing ATP-dependent RNA
FT                                helicase prp5.
FT                                /FTId=PRO_0000310227.
FT   DOMAIN      514    692       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      703    867       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     527    534       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       483    511       Q motif.
FT   MOTIF       640    643       DEAD box.
FT   COMPBIAS     26     72       Arg-rich.
SQ   SEQUENCE   1114 AA;  122005 MW;  2F42FA989B359915 CRC64;
     MADAIEIEEM MLEDRDIAVV LEVPTRRRRS RERDYRDRRD DSYDDRARRR REDSTDSWSR
     SRGDENRGQT PRSDSGAKVH DAPKASAPAA QTEEQKKAER LAKLEAWKKK MAEDKERKEK
     ELAAGGTRKL LDDIDQKANG SSSQPSPNTP TTPSTSAISG DLAPTNYAGK FDPKAIAKKA
     VASSSSAHAL GRDLPLKELS KTSATLTSTV KGLQADKKPT AFNSTSKVSA LPKTRGNLSV
     FGLGAKVTDN EKTYQKRALD FDEDEGSRKK LEKLPTLPMA NSKEDDAALA NGIEGQDDDD
     DAELEAAGTE EEAAAAARAA AEKREERLQE AESQPHTNGD VHMEDAPQAD STAMDEDEEI
     DPLDAFMEEM GDPFSLPKSN TTFVKNNTKS QPQEPEALFG DDDVDLKALD ADPDEILAIA
     NKARKKKDIP TINYANLNLP PFRKNFYTEP AELVDMTEAE INDLRLELDG IKVAGKDVPK
     PVQKWSQCGL DVKSLDVIKK LGYDKPTSIQ MQAIPAIMSG RDVIGVAKTG SGKTIAFLLP
     MFRHIRDQRP LKNSDGPIGL IMTPTRELAT QIHKECKPFL KAMGLRAVCA YGGAIIKDQI
     ADLKRGAEII VCTPGRMIEL LAANSGRVTN LQRVTYVVLD EADRMFDMGF EPQVMKVFNN
     IRPNRQTILF SATMPRIMDA LAKKTLQSPV EIVVGGRSVV APEITQIVEV REEKEKFHRL
     LELLGELYNA DEDARTLIFV DRQEKADDLL KDLMRKGYPC MSIHGGKDQV DRDSTIDDFK
     AGVVPIMIAT SVAARGLDVK QLKLVVNFDA PNHLEDYVHR AGRTGRAGNT GTAVTFITEE
     QEQYSVGIAK ALEQSGQEVP ERLNEMRKSY KDKVKSGAKK ESSGFGGKGL ERFDAEREAT
     KARERKIHKL NGDDDEEEKE EKDDDVLLKA ASVVQPASAS TAPPKLLGVP KGIDLDGDIK
     VHRTETAASS SGSKNPLDKV TSAIDAINAR LNKTGQLRSG VPIDNKGPDA GAFHATLEIN
     DFPQKARWAV TNRTNVAKIL EATGTSITTK GSFYPAGKEV QAGGDPKLYI LVEGDTEVVV
     TNAMRELMRL LKEGTMAAAD AEGRAPASGR YTVT
//
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