ID A7EWG9_SCLS1 Unreviewed; 4951 AA.
AC A7EWG9;
DT 11-SEP-2007, integrated into UniProtKB/TrEMBL.
DT 11-SEP-2007, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=SS1G_09678 {ECO:0000313|EMBL:EDN93811.1};
OS Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold)
OS (Whetzelinia sclerotiorum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Sclerotiniaceae; Sclerotinia.
OX NCBI_TaxID=665079 {ECO:0000313|EMBL:EDN93811.1, ECO:0000313|Proteomes:UP000001312};
RN [1] {ECO:0000313|Proteomes:UP000001312}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 18683 / 1980 / Ss-1 {ECO:0000313|Proteomes:UP000001312};
RX PubMed=21876677; DOI=.1371/journal.pgen.1002230;
RA Amselem J., Cuomo C.A., van Kan J.A., Viaud M., Benito E.P., Couloux A.,
RA Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E.,
RA Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.M.,
RA Quevillon E., Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P.,
RA Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I.,
RA Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P.,
RA Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C.,
RA Grossetete S., Guldener U., Henrissat B., Howlett B.J., Kodira C.,
RA Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E.,
RA Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N.,
RA Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E.,
RA Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA Zeng Q., Rollins J.A., Lebrun M.H., Dickman M.;
RT "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT sclerotiorum and Botrytis cinerea.";
RL PLoS Genet. 7:E1002230-E1002230(2011).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CH476634; EDN93811.1; -; Genomic_DNA.
DR RefSeq; XP_001589045.1; XM_001588995.1.
DR STRING; 665079.A7EWG9; -.
DR GeneID; 5485513; -.
DR KEGG; ssl:SS1G_09678; -.
DR VEuPathDB; FungiDB:sscle_01g000550; -.
DR InParanoid; A7EWG9; -.
DR OMA; ILEQWHR; -.
DR OrthoDB; 1221059at2759; -.
DR Proteomes; UP000001312; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IBA:GO_Central.
DR CDD; cd00009; AAA; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 9.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340};
KW Reference proteome {ECO:0000313|Proteomes:UP000001312}.
FT DOMAIN 4735..4939
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 752..771
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4095..4558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1422..1456
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 4126..4140
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4141..4155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4156..4201
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4202..4246
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4247..4265
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4271..4306
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4307..4329
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4342..4390
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4391..4406
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4437..4452
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4471..4485
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4497..4525
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4951 AA; 553450 MW; DCBA513E0FB97CD0 CRC64;
MEVVSNGNRP DIAMEANQQP NLPEEILEIV QLQQSPHFLE TLAISALKPK QTLKLFTYFN
TLIADSAARW VSNSHRGVQK LEIIEAFSRI LYLAPHLTTF LEKYLFETTH SAEYGVSRSI
LCLKDGVSAN GMSDLELQRI LLATWRLLNF DKQAYSGTIS GSTIQSLLGH KDIAVRYLAV
RIFCQLQFAS DDRLDAMVAK HVGNTRAVMG DFDGELVDYG FLSIFEEKRL KNAASELRTW
AGMTQFQKQP WEFSPLVVQY SDVLLPRPNG HPSQSSKLIS TITTSQNRES FAKALMSSAP
ILLHGLAGAG KTSLVNDFAR ELGMDSSMVT LHLNEQTDAK MLIGMYATGS TPGSFTWRAG
VLTTAVKEGR WVFIEDLDRA PNDVISVILP LVERGELLIP SRGETIKAKA GFKLLASIRT
SLNVSGGEIP PALHMLGARM WQRVPILMPA QDEFREIIEG TYPVLRQFLP GIISVYERLY
NLSVKPSLAS KNRTSSGRPI SPRDLLKWCR RLASVLTSSG SKTGSEPISD STKYEMFLEA
ADCFAGSLQT QDARGSLISC IAEEMHIDPQ TMEHFLDAHV PRYDDGENDL LIGRVRLSKK
KTSRVTKPIK KSRPFANTTH AKRLLEQVGV AVRMSEPVLL VGETGIGKTT VVQQLADSLG
FKLTAVNLSQ QSEVGDLLGG FKPVNVRSLA IPLKDEFDDL FTSTGISATK NQKYIDQLAK
CVAKSQWSKA SKLWREAPKM FEKIISELAK KEEANMRTNS EQPTKRRKTE SKLQSLLKLK
SRWSRFSQSL DQFDIQLSAG SKGFAFSFVE GNIVKAARNG EWVLLDEINL ASPDTLESIA
DLLHGGTGSP SILLSETGEI ERIQAHPDFR IFGAMNPATD VGKRDLPMGL RSRFTELYVD
SPDKNLDDLL AVIKAYLKGT SSNDDRAAHD VARLYMNTKR LAEEKRVVDG ANEVPHFSLR
TLTRVLSYVT EIAPSYGLRR ALYEGFAMGF LTLLDRESEK MLMPLIEHHL LDSHGNPQAL
LAQTPKHPDD GKQYVRFMNK KRDRQYWMLQ GYETPQEQPH YIITPFVERN MLNLVRATST
RRFPVLIQGP TSSGKTSMIE YLAKFSGNKF VRINNHEHTD LQEYLGTYVS DSDGKLRFQE
GLLVQALRQG HWIVLDELNL APTDVLEALN RLLDDNRELL IPETQEIVRP HENFMLFATQ
NPPGLYGGRK TLSRAFRNRF LELHFDDIPE DELDFILEKR SQKVAPSDCR RIVAVYKELS
RLRQSTRLFE QKDSFATLRD LFRWALRDAD NREQLAANGY MLLAERVRNS EERAAVKEII
ERVMKVKIDI DDLYSNGLAE IRAYNATTNS QGVVWTQAMR RLYVLVAHAL RNNEPVLLVG
ETGCGKTTVC QMLAEAFGKT LHIVNAHQNT ETGDLIGAQR PVRNRAAVLE QLKQDLKQLL
NEIGEEQKDS EDLDSLWAAY KSLPETTISQ IPTELSLRIQ LNQIKAKALF EWSDGSLVQA
LKEGQFFLLD EISLADDSVL ERLNSVLESH RTILLAEKGA EDSFVQAVEG FQFFATMNPG
GDYGKRELSP ALRNRFTEIW VPALSDHADV IQIVGAKLNP DLKQFSEPMV KFAEWFGQTY
RSTSSTSISV RDILAWIKFM NGCPTTDPYY AVLHGAAMVY IDTLGANPAA LLAINPETIQ
EERAKCLHQL GRLLNHDVST IYFMPIQVQD QDNHITMGGF SVPKQAGSDS DPGFAFDAPT
TKLNGMRVLR ALQVQKPILI EGSPGVGKTT LIAALARACN RPLTRINLSE QTDLMDLFGS
DVPVEGAEAG HFAWRDAPFL QAMQRGEWVL LDEMNLASQS VLEGLNACLD HRGEVYISEL
DQTFKRHPNF SVFAAQNPHH QGGGRKGLPS SFVNRFTVVY ADVFRNDDMK LICKHNFPSM
PQQLITTIID FVTRLEDEIV HKRKFGSQGG PWEFNLRDIL RWLHLLTSSA PFLTNRGPAD
FLNLIFRQRF RTIKDREELD GIFAQAFGFD VPFRQFFHNM SSTAYQVGIA YMPRHILYQR
TPFPQVEPVN RLPELESVIM CIQQNLPCIL VGSSGSGKST ILEHVAAASG KSLTVFPLNA
DIDTMDLVGG FEQVDPQRAA SRFLQELLEF MESRILGTLP EYVPDDAIKV LDILRKDTMN
SSFSFQEISG HLRQLEKASS LPEFGTLAET CETFASRPMA VENARFEWVD GVLIKALEQG
KWLVLDNANL CSASVLDRLN SLLEPNGFLS INEHCGPDGE PKIVKPHVDF RIFLTMDPRF
GELSRAMRNR AVEIFLQPLA LEIEPCKLRE FSIRPEASLE RYRQVSNILS IGDSNSRQAD
LINSIALDNL AWSDLPLLTR FSESVVRSLQ QQGLPTAKLT TISQQYVQLY QSGDCRELRN
AVGDLLNGVA KKTLLPAGFG DAQIIHPLQN SPVVPLLQSS SNQSQIYWVA ACLEYKLQIA
AALEAHQEQE SVIPTLKPSQ MNRLQRSIVS DRVASVAKDS TVQVSRFLMH TLKSINSYLN
AHLMETDHWK FRKQTITSLI RYWWSTFNLV TASTFEEVTF QSHLAIGNTV LEGLIKESPL
ADEQDSIIKG FVSDLQVNFA SGFKLTTGLS MELLWKKLRP VVTTKFEVMQ IVEQVEKLAV
RFDALRWRTS ISVAELGGIM ASFVKAHQLL LSSDVDGTAL IQSLTLELDN LEKSVDADEE
SPRPFLHDQF EALRQFQVLA ALASNKQPGD IVDVETVVLA DHPITSQMRL STSSPTSWLL
QNIDYLWSGE HGLQPISGSF STDFLHQMNV IDEVDLKSLR LLESELPVMA EKLARSSGVL
SSSQLAAINQ VLSNLMIGII GAHGEKSSND HLRRKEFFAL QASSKAWQSI TLDNFYEAVA
AHQAKHSSGQ PLVDTDMNFD IDNAMFAVEA ANNSTNSKMQ YSALAWVHFA VGCIALYVPD
RAFDPDKRQR LERQRHEEVI QGLEDKLDAL QKFERLFSGQ ESNLRCQLIG AEIAEIGEPP
EASQQVYRPE VSELDKLQGE FKNLLKVVLP SPAITSKIFQ YLINDDQDIL NEINLIQNNI
LQIIRRLSER FRAYSDLTTP VINILRCLQI GLYMAALVSK DPSNNSKAVL ALSKATPFLG
GTPKSIDEDI ASAQPLEYLT LMATTTSVEP IHSLDPQIRE SLFTSIHSCY MQWTKKLDAD
RLEGETKSGL YRFRGSAEDE DEIDQDQFNE LFPSYDEESP SASSAIPKQT NVRDIAVSLA
LVHEDIFLGG ALPSVSIMSL IKHISTHIGK LRGSDAAFED HNGVKAFLPG SLLLLSDKID
ALSSSATVPE NYNFYTDANL PETRKLVSLI HQVQTRFREL QSVDEIAHMQ PLEDVMVSCK
ELLQFRHTEP LAKIITKVEK VHGFMHEWQF GGWASRANSA LSLYDNLTAT IVSWRRLELS
TWAKLFDMEN RHCDDDARSW WFVAYQVIVA GPLSICESVE ELKAYAQKLL QDLEAYFSTA
IMGQYVQRLK LLKQLKKHVG LLVLDYPNLA IIGSALANFI ALYSRYEKSV EDTLKRGRVS
LEKAMRDVLL LASWKDTNIV ALRDSAKRSH HKLFKIVRKY RALLGQPMDS LIKQGLPDEV
VGDETGISYL APAQFPTVDQ SALALCASTV PSWTQKPKRF INVSKTVSMM VDKSQLPSST
VQGSSYLESF LENIITSTSE LQKATPSILT EDNKDTVKHL KSQKRKLFAD TLKDLRQMGI
KYNLGANALA KQDSLSIVLA NTDYIPSNDP QDSEYYFHKT VELAPRAREA SRQHSDDLSG
AEVARSTGFL EGLLQVLLSQ RNILSSTMTS LNLFRDVVKM AKGLWAPGIY DVRVPTTTSN
HKHVLQWLPN ILSVGLELVN THAKLGKIDA KSVVEILSSW KDILVDLRER WFSLSILPSG
IMSTQRREAE MAINDVTKQL SIDLSKLIEQ RPDLEFIIEQ ILPWTVIGES ATTAISTQAT
LEDLDQKLST VCNTVLAAME KYHKSIGDLP TSTEDQGWLI RSDTILANSI KALHSENVAD
QIKQSFAILR SLNLLDDNIS RGAGAIFAVA FPIIQQYYNI LEESITRYAI LHRSTSKTSY
ILAKTFTQIA SQGFCTPSEK QDAQDGKTEK LEGGTGLGDG EGAEDISKDI GEDEDLDELA
QEPNKEERGE IEDEKDAVDM ADGEMEGEMG EAEEKEDDEG SGDEEKDGDE MDEEAGDVDD
LDPNAVDEKM WDGDNEQAEK DQEGDDSKGK ASKDEQVAAQ DNNKEAGEGD EGEEAEEEEE
MAGAEQSEEV QQQDDVEKHD PHADEGDALD LPDDMQLDGE EKYGSDGSDD GMEDEMDDLP
DMEDEAKETD EVANDGKEEA DAENADGQED QDMGDEMDII DLDEDKDEEG EKTEEAGEKA
EEEPEEQEPE DQDGLLRGRE DEATVDTDDV VPSDVQGVGD GQDENEADEN ADSNSKAQRD
DGGQGGDSSE NKQSAAEDGE KGRQAAGDAP QDAQDDTQDS STAQPFKKLG DALEKWHRQQ
SQISESTEQK DQTQDQNIDM GTESNEFQHL QDEDAEADTQ ALGTATEEQA HALDESMAID
PEIGDMPETF QPDEVEKDED NVMDLENDAP EPEELSNAYE GRAGASIHQA KNNMDEDLND
NTRAQANENM DSDVEEVDTQ LDNTHLDPTT TISVRSAADA RAQWTHYESI TRPLSLSLTE
QLRLILAPTL ATKMRGDFRT GKRLNIKRII PYIASSFKRD KIWMRRSIPS KRSYQIMLAV
DDSKSMGESG SGSLALETLV MISRSLAMLE VGEICVVGFG EDVKVAHEFD VPFSSEAGPV
IFENFGFEQQ RTDITRLIRE SINLFRTARQ KASSSATDLW QISLIISDGV CSSSEHDNIR
RLLRQAEEER ILMVFVIVDD VRTKKKGESV LDLKEARFVK NEMTGQSDVR IERYLDTFPF
RYYIVVGDVG ELPGVLAGVL RQWFGEVVGS G
//
