ID ATG12_PICAN Reviewed; 167 AA.
AC A7KAJ7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 22-FEB-2023, entry version 50.
DE RecName: Full=Ubiquitin-like protein ATG12;
DE AltName: Full=Autophagy-related protein 12;
GN Name=ATG12;
OS Pichia angusta (Yeast) (Hansenula polymorpha).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Ogataea.
OX NCBI_TaxID=870730;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC Y-5445;
RX PubMed=17204848; DOI=10.4161/auto.3595;
RA Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W.;
RT "ATG genes involved in non-selective autophagy are conserved from yeast to
RT man, but the selective Cvt and pexophagy pathways also require organism-
RT specific genes.";
RL Autophagy 3:106-116(2007).
CC -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC transport (Cvt), autophagy vesicles formation, mitophagy, and
CC nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating
CC system involving also ATG7 as an E1-like activating enzyme and ATG10 as
CC an E2-like conjugating enzyme, is essential for its function. The
CC ATG12-ATG5 conjugate functions as an E3-like enzyme which is required
CC for lipidation of ATG8 and ATG8 association to the vesicle membranes
CC (By similarity). {ECO:0000250, ECO:0000269|PubMed:17204848}.
CC -!- SUBUNIT: Forms a conjugate with ATG5. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR EMBL; EF107719; ABO31057.1; -; Genomic_DNA.
DR AlphaFoldDB; A7KAJ7; -.
DR SMR; A7KAJ7; -.
DR PhylomeDB; A7KAJ7; -.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd01612; Ubl_ATG12; 1.
DR InterPro; IPR007242; Atg12.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13385; AUTOPHAGY PROTEIN 12; 1.
DR PANTHER; PTHR13385:SF0; UBIQUITIN-LIKE PROTEIN ATG12; 1.
DR Pfam; PF04110; APG12; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport; Transport;
KW Ubl conjugation pathway.
FT CHAIN 1..167
FT /note="Ubiquitin-like protein ATG12"
FT /id="PRO_0000317938"
FT CROSSLNK 167
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-135 in ATG5)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 167 AA; 18587 MW; 9DD9CBE36AF93575 CRC64;
MSDKDLASSL MELNIKVGTT DIPQTIAEEQ NQVTDTKEEV LDDETTIKND PQKTKITLNQ
SMMLSKLPAK TTSAVLSVSK PTESKIQIRF KSIGSVDQVS PAVFKISKSS KFSSILRFLE
LKLGKKVYCY LNNSVSPNPD EELENLYNIF RVGDELIVSY CNIVAFG
//
