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Database: UniProt
Entry: A7MSB2
LinkDB: A7MSB2
Original site: A7MSB2 
ID   RAPA_VIBCB              Reviewed;         969 AA.
AC   A7MSB2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   31-JUL-2019, entry version 73.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   OrderedLocusNames=VIBHAR_03676;
OS   Vibrio campbellii (strain ATCC BAA-1116 / BB120).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=338187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1116 / BB120;
RG   The Vibrio harveyi Genome Sequencing Project;
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N.,
RA   Pepin K., Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C.,
RA   Irgon J., Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; CP000789; ABU72590.1; -; Genomic_DNA.
DR   RefSeq; WP_012128985.1; NC_022269.1.
DR   EnsemblBacteria; ABU72590; ABU72590; VIBHAR_03676.
DR   GeneID; 5555339; -.
DR   KEGG; vca:M892_12820; -.
DR   KEGG; vha:VIBHAR_03676; -.
DR   PATRIC; fig|338187.25.peg.2559; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   HOGENOM; HOG000218482; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   OrthoDB; 291634at2; -.
DR   BioCyc; VCAM338187:G1HKJ-2601-MONOMER; -.
DR   Proteomes; UP000008152; Chromosome I.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 2.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    969       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_1000088398.
FT   DOMAIN      164    334       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      492    646       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     177    184       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       280    283       DEAH box.
SQ   SEQUENCE   969 AA;  110001 MW;  02A186DAD3DBC252 CRC64;
     MTFALGQRWI SDTESDLGLG TVVAMDARTV TLMFAASEEN RVYARNDAPV TRVTFNVGDV
     IDCQEGWSLK VEEVLEDEGL YTYFGTREDT QETAIVLREI FLSNHIRFNK PQDKLYAGQI
     DRMDNFVLRY RALQNQFEQH KSPMRGLCGM RAGLIPHQLY IAHEVGRRHA PRVLLADEVG
     LGKTIEAGMI IHQQVLSGRA ERILIVVPET LQHQWLVEMM RRFNLHFSIF DEERCIEAFA
     EAENPFDTQQ YVLCSLDFLR KSRKRFEQAL EGEWDLLVVD EAHHLEWSQD KPSREYQVVE
     GLAERTAGVL LLTATPEQLG RESHFARLRL LDPDRFYDYD AFVEEEEQYA PVADAITSLF
     SGEKLPDEAK NQITELLSEQ DVEPLFRIIE SDSDEEAKAS ARQELIDNLM DRHGTGRVLF
     RNTRAAIKGF PKRNVHLMPM DIPQQYTTSM RVSGMIGGKM TPEARAVKNL YPEEIFQEFE
     GEDSSWWQFD SRVNWLLEKV KEKRSDKVLV IASRASTALQ LEQALREREG IRATVFHEGM
     SILERDKAAA YFAQEEGGAQ VLICSEIGSE GRNFQFANQL VMFDLPFNPD LLEQRIGRLD
     RIGQKRDIDI HVPYLKDTSQ AILARWFDEG LNAFAETCPT GRAVYDKYSD ALIEMLASGD
     VSNLDEVIEE SAKMNKELKS QLEQGRDRLL EMHSNGGEKA QQIVEKISST DGDTNLVTFA
     LSLFDTIGLN QDDKGENALV VTPSEHMMVP SYPGLPYEGA TITFDRDTAL SREDMHFISW
     EHPMIQGGID LLMSEGVGTS AVSLLKNKAL PVGTILLELV YAVDAQAPKR SGITRFLPKT
     PIRMMMDARG NDLSPQVEFE GFNRQLSPVN RHLGSKLVTS VQKDIHSLIE AGDALVEQKV
     EEVRKQAHQD MQQSLNAELE RLQALKAVNP NIRDEEIESI EEQIKELTGY INQAQVQLDS
     LRLIVVSHN
//
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