ID A7RV47_NEMVE Unreviewed; 422 AA.
AC A7RV47;
DT 02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 02-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 {ECO:0000256|ARBA:ARBA00021134};
DE EC=2.1.1.296 {ECO:0000256|ARBA:ARBA00012770};
DE Flags: Fragment;
GN ORFNames=NEMVEDRAFT_v1g163068 {ECO:0000313|EMBL:EDO44686.1};
OS Nematostella vectensis (Starlet sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Edwardsiidae; Nematostella.
OX NCBI_TaxID=45351 {ECO:0000313|EMBL:EDO44686.1, ECO:0000313|Proteomes:UP000001593};
RN [1] {ECO:0000313|EMBL:EDO44686.1, ECO:0000313|Proteomes:UP000001593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CH2 X CH6 {ECO:0000313|Proteomes:UP000001593};
RX PubMed=17615350; DOI=10.1126/science.1139158;
RA Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA Technau U., Martindale M.Q., Rokhsar D.S.;
RT "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT genomic organization.";
RL Science 317:86-94(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-
CC ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine =
CC a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-
CC ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + H(+) + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:67024, Rhea:RHEA-COMP:17169,
CC Rhea:RHEA-COMP:17170, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:167612, ChEBI:CHEBI:167614;
CC EC=2.1.1.296; Evidence={ECO:0000256|ARBA:ARBA00024560};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS469542; EDO44686.1; -; Genomic_DNA.
DR RefSeq; XP_001636749.1; XM_001636699.1.
DR AlphaFoldDB; A7RV47; -.
DR STRING; 45351.A7RV47; -.
DR EnsemblMetazoa; EDO44686; EDO44686; NEMVEDRAFT_v1g163068.
DR GeneID; 5516727; -.
DR KEGG; nve:5516727; -.
DR eggNOG; KOG3674; Eukaryota.
DR HOGENOM; CLU_019427_1_1_1; -.
DR InParanoid; A7RV47; -.
DR OMA; MFEERTI; -.
DR OrthoDB; 5488054at2759; -.
DR PhylomeDB; A7RV47; -.
DR Proteomes; UP000001593; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProt.
DR GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IEA:UniProt.
DR Gene3D; 3.40.50.12760; -; 1.
DR InterPro; IPR025807; Adrift-typ_MeTrfase.
DR InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR16121:SF2; CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2; 1.
DR PANTHER; PTHR16121; UNCHARACTERIZED; 1.
DR Pfam; PF01728; FtsJ; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51614; SAM_MT_ADRIFT; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|PROSITE-ProRule:PRU00946};
KW Reference proteome {ECO:0000313|Proteomes:UP000001593};
KW S-adenosyl-L-methionine {ECO:0000256|PROSITE-ProRule:PRU00946};
KW Transferase {ECO:0000256|PROSITE-ProRule:PRU00946}.
FT DOMAIN 114..325
FT /note="Adrift-type SAM-dependent 2'-O-MTase"
FT /evidence="ECO:0000259|PROSITE:PS51614"
FT ACT_SITE 278
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 153
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 172
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 238
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT NON_TER 422
FT /evidence="ECO:0000313|EMBL:EDO44686.1"
SQ SEQUENCE 422 AA; 48525 MW; B5852C92AB98DBFC CRC64;
MAPKRRNKQR YHPYGESALL SAETLQFIEE ETTRLYNKRF TLKAKDKQCE LPSSELLFTT
TPETINVLQE MKRNLNESKS MLNDIDIVEW HQHTRRTHKA GLVVKTLRET MRAEMCTQAF
AKLYEVLASY NVVPTEAVSS KKLYGVHLCE APGAFVAATN HYLRNHHPDL EWVWLGATLN
PYYEGHSPKA LLDDDRFIVE TLQNWDFGAD GTGDLMQLEN LNHLAEACQG KVHLVTADGS
IDCSDEPEEQ ENAVSQLIFC EAVTALTVLA TGGCYVFKMF TTLEHHMVCL MYLMCCLFEE
VHVIKPGTSK GGNSEVYITC CGYTGNLSPQ YHEILYTAYQ QDTAEKVLFP LNSIPREFLY
LIKECQEYFI NLQNQEISMN IQYFHHMNRL EKKQLFHLRK QAAGYYMEKF GVQPIAEEER
VV
//
