ID A7TE15_VANPO Unreviewed; 816 AA.
AC A7TE15;
DT 02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 02-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE SubName: Full=Tkp5 protein {ECO:0000313|EMBL:EDO19635.1};
GN ORFNames=Kpol_1018p175 {ECO:0000313|EMBL:EDO19635.1};
OS Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX NCBI_TaxID=436907 {ECO:0000313|Proteomes:UP000000267};
RN [1] {ECO:0000313|EMBL:EDO19635.1, ECO:0000313|Proteomes:UP000000267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 /
RC NRRL Y-8283 / UCD 57-17 {ECO:0000313|Proteomes:UP000000267};
RX PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT species descended from a whole-genome duplication.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC -!- FUNCTION: Integrase (IN) targets the VLP to the nucleus, where a
CC subparticle preintegration complex (PIC) containing at least integrase
CC and the newly synthesized dsDNA copy of the retrotransposon must
CC transit the nuclear membrane. Once in the nucleus, integrase performs
CC the integration of the dsDNA into the host genome.
CC {ECO:0000256|ARBA:ARBA00025615}.
CC -!- FUNCTION: Reverse transcriptase/ribonuclease H (RT) is a
CC multifunctional enzyme that catalyzes the conversion of the retro-
CC elements RNA genome into dsDNA within the VLP. The enzyme displays a
CC DNA polymerase activity that can copy either DNA or RNA templates, and
CC a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-
CC DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA
CC primers. The conversion leads to a linear dsDNA copy of the
CC retrotransposon that includes long terminal repeats (LTRs) at both
CC ends. {ECO:0000256|ARBA:ARBA00025590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000077};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49;
CC Evidence={ECO:0000256|ARBA:ARBA00024557};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; DS480378; EDO19635.1; -; Genomic_DNA.
DR RefSeq; XP_001647493.1; XM_001647443.1.
DR AlphaFoldDB; A7TE15; -.
DR STRING; 436907.A7TE15; -.
DR GeneID; 5548000; -.
DR KEGG; vpo:Kpol_1018p175; -.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_346197_0_0_1; -.
DR InParanoid; A7TE15; -.
DR OMA; CRSITTS; -.
DR OrthoDB; 1909432at2759; -.
DR PhylomeDB; A7TE15; -.
DR Proteomes; UP000000267; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR035179; DUF5314.
DR InterPro; IPR025724; GAG-pre-integrase_dom.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR42648; TRANSPOSASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR42648:SF11; TRANSPOSON TY4-P GAG-POL POLYPROTEIN; 1.
DR Pfam; PF13976; gag_pre-integrs; 1.
DR Pfam; PF17241; Retrotran_gag_4; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Reference proteome {ECO:0000313|Proteomes:UP000000267};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transposable element {ECO:0000256|ARBA:ARBA00022464};
KW Transposition {ECO:0000256|ARBA:ARBA00022578}.
FT DOMAIN 512..682
FT /note="Integrase catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50994"
SQ SEQUENCE 816 AA; 91159 MW; 859275B08008177B CRC64;
MDQHINNNSF ETANSTFSVW NLRQQVMSPE AVNDTVIPIK LLGVNNFNSW LQDLTDHVSE
INPIWGDYVE TGNIHSITQE FNLTTDTVNS IISTLDKALR RLIVKSISPE VKIKVDEYYQ
NKFRTRNSLE LLSYLEDLYG KKCVQHIAPV YFEMNSIHSK SLEEKTIWTR KLVKSMFDLD
QLDDEIPDPT MKNTVTEYVR KLQDSAAAMV LMGITPDMKE RFFDLFGQQD TVTFKEVYSM
IKNSRSSASD VTATPLVAKK KWYGNKQKHL CKTGHYNVTD SISNAQVASV WMATEFQGLC
YNSSTLSSPS LTSEDKSIWV LDTGSSTHIS CNRESFSNFR PSTNNGTISG VGVAVKIRGY
GDVMVGELTL CDVAYVPDLP INLISIRQAS KKSGCSFSFD ETGAYVLSAN NKLTKIGSLY
KKLYVLDQSL SKIEETQMSF VAISDPLLTS DVKPVSTAIR WHARFGHPGL DRYNTVARQF
SLPRIVPEQV SVCPTCALAK GVMRKGKPSD TKLTCPLQLV QVGLCGGFRY KEFKDNKYFM
TIRDAFTRYY VVIHLKDKKD APVQLINWIK KTEKYFASRG GYRVGSLCTN NGGEFRNKLL
HDFFIEQGIT HELTIPYDSF QNGGIERGHR TIEETTRCLL ISGRVPPSLW SEAVSCAVYL
INRIPVTSRQ NKIPVAEWYQ MKLPLDGFSH LRTFGCAAFA TIPHQLGDGK FSPATINGVM
VGYDSEQKGY RIFHPESGNV FVSNQVTFDE SSFPLEGLDV NVDAHKIATG TLNGSPSSSS
SASTFTGGTL SSVFLTDFSH SHPPSSPNKG TSKGYI
//
