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Database: UniProt
Entry: A7TK63
LinkDB: A7TK63
Original site: A7TK63 
ID   DBP8_VANPO              Reviewed;         431 AA.
AC   A7TK63;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   16-OCT-2019, entry version 57.
DE   RecName: Full=ATP-dependent RNA helicase DBP8;
DE            EC=3.6.4.13;
GN   Name=DBP8; ORFNames=Kpol_1062p5;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294)
OS   (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
OC   Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M.,
RA   Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two
RT   yeast species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in 40S ribosomal
CC       subunit biogenesis and is required for the normal formation of 18S
CC       rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required
CC       for vegetative growth (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX49/DBP8
CC       subfamily. {ECO:0000305}.
DR   EMBL; DS480406; EDO17298.1; -; Genomic_DNA.
DR   RefSeq; XP_001645156.1; XM_001645106.1.
DR   SMR; A7TK63; -.
DR   STRING; 36033.XP_001645156.1; -.
DR   PRIDE; A7TK63; -.
DR   EnsemblFungi; EDO17298; EDO17298; Kpol_1062p5.
DR   GeneID; 5545504; -.
DR   KEGG; vpo:Kpol_1062p5; -.
DR   eggNOG; KOG0340; Eukaryota.
DR   eggNOG; ENOG410XPYC; LUCA.
DR   InParanoid; A7TK63; -.
DR   KO; K14778; -.
DR   OMA; TEHPIDQ; -.
DR   OrthoDB; 744428at2759; -.
DR   PhylomeDB; A7TK63; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    431       ATP-dependent RNA helicase DBP8.
FT                                /FTId=PRO_0000310237.
FT   DOMAIN       33    209       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      242    389       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      46     53       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF         2     30       Q motif.
FT   MOTIF       155    158       DEAD box.
SQ   SEQUENCE   431 AA;  47794 MW;  4B684CBEC8AD958D CRC64;
     MQDFKSLGLS RWLVESLNAM RITHPTAIQK HCIPEILKGR DCIGGAKTGS GKTIAFAGPM
     LSQWSDDPSG MFGVVLTPTR ELAIQIAEQF TALGSSMNIR VCLVVGGESI VKQALELQKK
     PHFIIATPGR LAHHILSSGE EVVGGLSRVK YLVLDEADLI LTQTFAADLS TCIAKLPPKQ
     KRQTLLFTAT ITDQVRALQN APAQDSKPPL FAYEVENVDN VAVPSTLKLE YLLVPEHVKE
     AYLYQLLTCE DYKDSSVIVF VNRTTAAEVL RRTLRSLEVR VASLHSQMPQ SERINSLQRF
     RANAARVLIA TDVAARGLDI PTVELVINYD IPQDPDTFIH RSGRTARAGR SGDAISFVTP
     RDVSRIEAIE ERINKKMDEC KKVHDTAVIR KALTKVTKAK RESLMDMEKA NFGEKRKTQK
     KKNLAEKSLR A
//
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