ID   A8ADN2_CITK8            Unreviewed;       520 AA.
AC   A8ADN2;
DT   23-OCT-2007, integrated into UniProtKB/TrEMBL.
DT   23-OCT-2007, sequence version 1.
DT   27-MAR-2024, entry version 82.
DE   RecName: Full=diguanylate cyclase {ECO:0000256|ARBA:ARBA00012528};
DE            EC=2.7.7.65 {ECO:0000256|ARBA:ARBA00012528};
GN   OrderedLocusNames=CKO_00438 {ECO:0000313|EMBL:ABV11595.1};
OS   Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=290338 {ECO:0000313|EMBL:ABV11595.1, ECO:0000313|Proteomes:UP000008148};
RN   [1] {ECO:0000313|EMBL:ABV11595.1, ECO:0000313|Proteomes:UP000008148}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696
RC   {ECO:0000313|Proteomes:UP000008148};
RG   The Citrobacter koseri Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA   Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA   Johnson M., Thiruvilangam P., Wilson R.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 GTP = 3',3'-c-di-GMP + 2 diphosphate; Xref=Rhea:RHEA:24898,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:58805; EC=2.7.7.65;
CC         Evidence={ECO:0000256|ARBA:ARBA00034247};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- PATHWAY: Purine metabolism; 3',5'-cyclic di-GMP biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00004665}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; CP000822; ABV11595.1; -; Genomic_DNA.
DR   RefSeq; WP_012131422.1; NC_009792.1.
DR   AlphaFoldDB; A8ADN2; -.
DR   STRING; 290338.CKO_00438; -.
DR   GeneID; 45134690; -.
DR   KEGG; cko:CKO_00438; -.
DR   HOGENOM; CLU_000445_134_6_6; -.
DR   OrthoDB; 9812260at2; -.
DR   Proteomes; UP000008148; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   CDD; cd01949; GGDEF; 1.
DR   CDD; cd18773; PDC1_HK_sensor; 1.
DR   CDD; cd12912; PDC2_MCP_like; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   Gene3D; 3.30.450.20; PAS domain; 1.
DR   InterPro; IPR033479; dCache_1.
DR   InterPro; IPR000160; GGDEF_dom.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR029151; Sensor-like_sf.
DR   NCBIfam; TIGR00254; GGDEF; 1.
DR   PANTHER; PTHR45138:SF9; DIGUANYLATE CYCLASE DGCM-RELATED; 1.
DR   PANTHER; PTHR45138; REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEM; 1.
DR   Pfam; PF02743; dCache_1; 1.
DR   Pfam; PF00990; GGDEF; 1.
DR   SMART; SM00267; GGDEF; 1.
DR   SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR   SUPFAM; SSF103190; Sensory domain-like; 2.
DR   PROSITE; PS50887; GGDEF; 1.
PE   4: Predicted;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008148};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        282..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          392..520
FT                   /note="GGDEF"
FT                   /evidence="ECO:0000259|PROSITE:PS50887"
SQ   SEQUENCE   520 AA;  58108 MW;  5E824D77B59D2B43 CRC64;
     MKKKLLQMLN LRLLIIAFAF LMTLLTLFNS FYSAWRVQKQ VLIENELSEN QAYAERIAST
     IDLYLAVFME RLEYSASVIG QHFDDESIIS GEMMRMQRRD FGFDSVTLVD EKGKIIGVLP
     ESLNLKGKML TTPGGVEALK EMQPTVSSAY NSLSGNLVVY ISHPVRNAQG KYLGFIGGAI
     YLQRSNVLSA LISNHFRNDD SYIYVVDKND QLLYHPDPAR RGEKEEGNAA VKAVEQGLSG
     AMEVTNSRGV EMLAGYASIN TSGWGVVAQK PKDKTLTALD GLMWQMIRGV VPLGIVGLFI
     LWWLGNEIVR PLRQLADHAI SMDSDESAEK VRNVRSWYIE ASRLRRALLQ GMELIREKMK
     WLNDQASTDP LTGLVNRRAA EKLLAEYEHQ QRQFAVISLD IDRFKAVNDT FGHDIGDVTL
     KALAQLLKRS CRKNDTACRM GGEEFMMFFP ETSVEAAADI AERLRKEVAA AKIETVGHIT
     ISLGVTVWPS GVDSVSQALK RADELMYQAK QAGRNQVVAG
//