ID A8CWB5_LUTLO Unreviewed; 433 AA.
AC A8CWB5;
DT 13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2007, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=Enolase {ECO:0000256|ARBA:ARBA00017068};
DE EC=4.2.1.11 {ECO:0000256|ARBA:ARBA00012058};
DE AltName: Full=2-phospho-D-glycerate hydro-lyase {ECO:0000256|ARBA:ARBA00031125};
DE AltName: Full=2-phosphoglycerate dehydratase {ECO:0000256|ARBA:ARBA00032132};
OS Lutzomyia longipalpis (Sand fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Psychodoidea; Psychodidae;
OC Lutzomyia; Lutzomyia.
OX NCBI_TaxID=7200 {ECO:0000313|EMBL:ABV60328.1};
RN [1] {ECO:0000313|EMBL:ABV60328.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Midgut {ECO:0000313|EMBL:ABV60328.1};
RX PubMed=18194529; DOI=10.1186/1471-2164-9-15;
RA Jochim R.C., Teixeira C.R., Laughinghouse A., Mu J., Oliveira F.,
RA Gomes R.B., Elnaiem D.E., Valenzuela J.G.;
RT "The midgut transcriptome of Lutzomyia longipalpis: comparative analysis of
RT cDNA libraries from sugar-fed, blood-fed, post-digested and Leishmania
RT infantum chagasi-infected sand flies.";
RL BMC Genomics 9:15-15(2008).
RN [2] {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA, ECO:0000313|Proteomes:UP000092461}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Jacobina {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA,
RC ECO:0000313|Proteomes:UP000092461};
RA Richards S., Qu C., Dillon R., Worley K., Scherer S., Batterton M.,
RA Taylor A., Hawes A., Hernandez B., Kovar C., Mandapat C., Pham C., Qu C.,
RA Jing C., Bess C., Bandaranaike D., Ngo D., Ongeri F., Arias F., Lara F.,
RA Weissenberger G., Kamau G., Han H., Shen H., Dinh H., Khalil I., Jones J.,
RA Shafer J., Jayaseelan J., Quiroz J., Blankenburg K., Nguyen L., Jackson L.,
RA Francisco L., Tang L.-Y., Pu L.-L., Perales L., Lorensuhewa L.,
RA Munidasa M., Coyle M., Taylor M., Puazo M., Firestine M., Scheel M.,
RA Javaid M., Wang M., Li M., Tabassum N., Saada N., Osuji N., Aqrawi P.,
RA Fu Q., Thornton R., Raj R., Goodspeed R., Mata R., Najjar R., Gubbala S.,
RA Lee S., Denson S., Patil S., Macmil S., Qi S., Matskevitch T.,
RA Palculict T., Mathew T., Vee V., Velamala V., Korchina V., Cai W., Liu W.,
RA Dai W., Zou X., Zhu Y., Zhang Y., Wu Y.-Q., Xin Y., Nazarath L., Kovar C.,
RA Han Y., Muzny D., Gibbs R.;
RT "Whole Genome Assembly of Lutzomyia longipalpis.";
RL Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA}
RP IDENTIFICATION.
RC STRAIN=Jacobina {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA};
RG VectorBase;
RL Submitted (JUL-2016) to UniProtKB.
RN [4] {ECO:0000313|EMBL:MBC1171544.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Jacobina {ECO:0000313|EMBL:MBC1171544.1};
RC TISSUE=Midgut {ECO:0000313|EMBL:MBC1171544.1};
RA Coutinho-Abreu I.V., Serafim T.D., Meneses C., Kamhawi S., Oliveira F.,
RA Valenzuela J.G.;
RT "Leishmania infection induces a limited differential gene expression in the
RT sand fly midgut.";
RL BMC 21:608-608(2020).
RN [5] {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA}
RP IDENTIFICATION.
RC STRAIN=Jacobina {ECO:0000313|EnsemblMetazoa:LLOJ000219-PA};
RG EnsemblMetazoa;
RL Submitted (JAN-2021) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 4/5. {ECO:0000256|ARBA:ARBA00005031}.
CC -!- SIMILARITY: Belongs to the enolase family.
CC {ECO:0000256|ARBA:ARBA00009604}.
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DR EMBL; AJWK01000758; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; EU124610; ABV60328.1; -; mRNA.
DR EMBL; GITU01002841; MBC1171544.1; -; Transcribed_RNA.
DR AlphaFoldDB; A8CWB5; -.
DR EnsemblMetazoa; LLOJ000219-RA; LLOJ000219-PA; LLOJ000219.
DR VEuPathDB; VectorBase:LLOJ000219; -.
DR OrthoDB; 1093250at2759; -.
DR UniPathway; UPA00109; UER00187.
DR Proteomes; UP000092461; Unassembled WGS sequence.
DR GO; GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0004634; F:phosphopyruvate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03313; enolase; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR HAMAP; MF_00318; Enolase; 1.
DR InterPro; IPR000941; Enolase.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR020810; Enolase_C.
DR InterPro; IPR020809; Enolase_CS.
DR InterPro; IPR020811; Enolase_N.
DR NCBIfam; TIGR01060; eno; 1.
DR PANTHER; PTHR11902; ENOLASE; 1.
DR PANTHER; PTHR11902:SF1; ENOLASE; 1.
DR Pfam; PF00113; Enolase_C; 1.
DR Pfam; PF03952; Enolase_N; 1.
DR PIRSF; PIRSF001400; Enolase; 1.
DR PRINTS; PR00148; ENOLASE.
DR SFLD; SFLDF00002; enolase; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SMART; SM01192; Enolase_C; 1.
DR SMART; SM01193; Enolase_N; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
DR PROSITE; PS00164; ENOLASE; 1.
PE 2: Evidence at transcript level;
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842}.
FT DOMAIN 3..134
FT /note="Enolase N-terminal"
FT /evidence="ECO:0000259|SMART:SM01193"
FT DOMAIN 142..432
FT /note="Enolase C-terminal TIM barrel"
FT /evidence="ECO:0000259|SMART:SM01192"
FT ACT_SITE 210
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT ACT_SITE 344
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT BINDING 158
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 167
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 294
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 319
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 371..374
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 395
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
SQ SEQUENCE 433 AA; 46762 MW; E6584F929251DCFB CRC64;
MVIQKIVARQ IFDSRGNPTV EVDLTTNLGM FRAAVPSGAS TGVHEALELR DNDKNNYHGK
GVLKAVENIN KIIAPEVIKS GICVTAQEDM DNFMLKLDGT ENKSKFGANA ILGVSLAVCK
AGAAAKGVPL YQHIANLAGN PNIILPVPAF NVINGGSHAG NKLAMQEFMI LPTGATSFTE
AMKIGSEVYH HLKKVINNKF GLDATAVGDE GGFAPNILNN KDALFLIQDA IAKAGYTGKV
EIGMDVAASE FHKDGLYDLD FKNPNSDKSL WLSPDKLTEL YMEFVKEFPI TSIEDPFDQD
HWDAWSKMTA GTNIQIVGDD LTVTNPKRIA TAVEKKACNC LLLKVNQIGT VTESIKAHNL
AKSNGWGTMV SHRSGETEDT FIADLVVGLS TGQIKTGAPC RSERLAKYNQ ILRIEEELGA
GAKFAGKSFR KPQ
//
