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Database: UniProt
Entry: A8F8W9
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ID   UVRB_PSELT              Reviewed;         656 AA.
AC   A8F8W9;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   31-JUL-2019, entry version 78.
DE   RecName: Full=UvrABC system protein B {ECO:0000255|HAMAP-Rule:MF_00204};
DE            Short=Protein UvrB {ECO:0000255|HAMAP-Rule:MF_00204};
DE   AltName: Full=Excinuclease ABC subunit B {ECO:0000255|HAMAP-Rule:MF_00204};
GN   Name=uvrB {ECO:0000255|HAMAP-Rule:MF_00204};
GN   OrderedLocusNames=Tlet_2049;
OS   Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC
OS   107922 / TMO) (Thermotoga lettingae).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae;
OC   Pseudothermotoga.
OX   NCBI_TaxID=416591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA   Dalin E., Tice H., Pitluck S., Foster B., Bruce D., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nelson K.,
RA   Gogarten J.P., Noll K., Richardson P.;
RT   "Complete sequence of Thermotoga lettingae TMO.";
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC       processing of DNA lesions. A damage recognition complex composed
CC       of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC       binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC       DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC       binding by UvrB and probably causes local melting of the DNA
CC       helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC       strands. Then UvrB probes one DNA strand for the presence of a
CC       lesion. If a lesion is found the UvrA subunits dissociate and the
CC       UvrB-DNA preincision complex is formed. This complex is
CC       subsequently bound by UvrC and the second UvrB is released. If no
CC       lesion is found, the DNA wraps around the other UvrB subunit that
CC       will check the other stand for damage. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC       lesions. Interacts with UvrC in an incision complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- DOMAIN: The beta-hairpin motif is involved in DNA binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SIMILARITY: Belongs to the UvrB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
DR   EMBL; CP000812; ABV34603.1; -; Genomic_DNA.
DR   RefSeq; WP_012004079.1; NC_009828.1.
DR   SMR; A8F8W9; -.
DR   STRING; 416591.Tlet_2049; -.
DR   EnsemblBacteria; ABV34603; ABV34603; Tlet_2049.
DR   KEGG; tle:Tlet_2049; -.
DR   eggNOG; ENOG4105CCW; Bacteria.
DR   eggNOG; COG0556; LUCA.
DR   HOGENOM; HOG000073580; -.
DR   KO; K03702; -.
DR   OMA; RYMHSEI; -.
DR   OrthoDB; 95696at2; -.
DR   BioCyc; TLET416591:G1G9T-2117-MONOMER; -.
DR   Proteomes; UP000002016; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00204; UvrB; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   InterPro; IPR036876; UVR_dom_sf.
DR   InterPro; IPR004807; UvrB.
DR   InterPro; IPR041471; UvrB_inter.
DR   InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR   PANTHER; PTHR24029; PTHR24029; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF02151; UVR; 1.
DR   Pfam; PF12344; UvrB; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46600; SSF46600; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00631; uvrb; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW   DNA repair; Excision nuclease; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN         1    656       UvrABC system protein B.
FT                                /FTId=PRO_1000077939.
FT   DOMAIN       23    180       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      426    588       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      614    649       UVR. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   NP_BIND      36     43       ATP. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   MOTIF        89    112       Beta-hairpin.
SQ   SEQUENCE   656 AA;  75808 MW;  74A9ADE5BAC2C78F CRC64;
     MFKLVSDYEP TGDQPQAIDR LVEGIKKGYR FQTLIGVTGS GKTFTMANVI SRLNRPALVI
     SPNKTLAAQL YGEFKTFFPH NRVEYFISYY DYYQPEAYVP TKDLYIEKNA DINDVLVRMR
     MSALKSVRTR RDVVVVASVS SIYASGDPSD FDRMNIQLST GLKISPHAVA QHLAKIGYER
     SNEITVKGCF RLRGDVLEIY PTYQDEGIRI EFFGSVVDRI ETFDKLNRSP LEELQKIIIY
     PAIEFVTTEE KLKRAVESIK NELDQRLTEL KNQGKILEAQ RLQQRTMHDL ELLSALGYCP
     GIENYSRHFD GRKPGEPPYT LLDYFDDDVL VFLDESHIAV PQLRAMWRGE HSRKKSLVEY
     GFRLPSAFDN RPLTFEEFLK KVPQIIFVSA TPGPFEYQVS EQIVEQIIRP TGLIDPEVEV
     RPTKYQVDDL ISEIKKVVER GERALITVLT KKTAEKLSEY LVEMGIKSLY IHSELDAIER
     IEVLKKLRRG DVDAVVGINL LREGLDLPEV SLVAILDSDK EGFLRSETTL IQIIGRVARN
     LNGKVLMYAD RVTPAMQRAI DETNRRRKIQ MEYNEKYGIT PKTIVKPLQI EIFEKFMEKP
     EIDYHQLAKD LSKEEYLSLL EEEMYRAASE LRYEDAAKLR DEIFRLREEL KDENYL
//
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