ID A8JCP5_CHLRE Unreviewed; 1094 AA.
AC A8JCP5;
DT 04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT 04-DEC-2007, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=proton-translocating NAD(P)(+) transhydrogenase {ECO:0000256|ARBA:ARBA00012943};
DE EC=7.1.1.1 {ECO:0000256|ARBA:ARBA00012943};
GN ORFNames=CHLRE_01g054500v5 {ECO:0000313|EMBL:PNW89009.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000313|EMBL:PNW89009.1, ECO:0000313|Proteomes:UP000006906};
RN [1] {ECO:0000313|EMBL:PNW89009.1, ECO:0000313|Proteomes:UP000006906}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503 {ECO:0000313|Proteomes:UP000006906};
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+);
CC Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349; EC=7.1.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000006};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CM008962; PNW89009.1; -; Genomic_DNA.
DR RefSeq; XP_001700222.1; XM_001700170.1.
DR AlphaFoldDB; A8JCP5; -.
DR SMR; A8JCP5; -.
DR STRING; 3055.A8JCP5; -.
DR PaxDb; 3055-EDO98337; -.
DR EnsemblPlants; PNW89009; PNW89009; CHLRE_01g054500v5.
DR GeneID; 5725773; -.
DR Gramene; PNW89009; PNW89009; CHLRE_01g054500v5.
DR KEGG; cre:CHLRE_01g054500v5; -.
DR eggNOG; ENOG502QQ0A; Eukaryota.
DR HOGENOM; CLU_003376_0_0_1; -.
DR InParanoid; A8JCP5; -.
DR OMA; EQCREVD; -.
DR OrthoDB; 313696at2759; -.
DR Proteomes; UP000006906; Chromosome 1.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008746; F:NAD(P)+ transhydrogenase activity; IBA:GO_Central.
DR GO; GO:0050661; F:NADP binding; IBA:GO_Central.
DR GO; GO:0006740; P:NADPH regeneration; IBA:GO_Central.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR CDD; cd05304; Rubrum_tdh; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR008143; Ala_DH/PNT_CS2.
DR InterPro; IPR007886; AlaDH/PNT_N.
DR InterPro; IPR007698; AlaDH/PNT_NAD(H)-bd.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR026255; NADP_transhyd_a.
DR InterPro; IPR024605; NADP_transhyd_a_C.
DR InterPro; IPR034300; PNTB-like.
DR NCBIfam; TIGR00561; pntA; 1.
DR PANTHER; PTHR10160; NAD(P) TRANSHYDROGENASE; 1.
DR PANTHER; PTHR10160:SF19; PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE; 1.
DR Pfam; PF01262; AlaDh_PNT_C; 1.
DR Pfam; PF05222; AlaDh_PNT_N; 1.
DR Pfam; PF02233; PNTB; 1.
DR Pfam; PF12769; PNTB_4TM; 1.
DR SMART; SM01002; AlaDh_PNT_C; 1.
DR SMART; SM01003; AlaDh_PNT_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52283; Formate/glycerate dehydrogenase catalytic domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00837; ALADH_PNT_2; 1.
PE 4: Predicted;
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW NAD {ECO:0000256|ARBA:ARBA00023027}; NADP {ECO:0000256|ARBA:ARBA00022857};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000006906};
KW Translocase {ECO:0000256|ARBA:ARBA00022967};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 72..207
FT /note="Alanine dehydrogenase/pyridine nucleotide
FT transhydrogenase N-terminal"
FT /evidence="ECO:0000259|SMART:SM01003"
FT DOMAIN 216..381
FT /note="Alanine dehydrogenase/pyridine nucleotide
FT transhydrogenase NAD(H)-binding"
FT /evidence="ECO:0000259|SMART:SM01002"
SQ SEQUENCE 1094 AA; 112347 MW; 1A869D2911A4122F CRC64;
MLSMLGRKGQ AMCLRSSRQV PSTRASIARL LAQTPRPNVR ANALAGTAPG TSTSGPAEPI
KDPIPYANLV VGVPRENAPG ERRVALSPAA VAGLLKQGFK TIVVEKGAGE YSQFTDAEYA
SAGATLVDST EAMGADIVLR VRPPALEEVN KIKEGAVLVS YLYPARNKEL VEALAKRRAT
AIGMDCIPRT ISRAQMFDSL SSMANIAGYR AVTEATQHFE RFLNGQITAA GRIPPAKVLV
IGGGVAGLAA VGAAKSLGAI VRVFDTRTAV KEQAKSMGAE FLTVDIQEEG ESGTGYSKEM
SPAFIEAEMK LFAAQAKEVD IIITTALIPG KGAPLLITKD MVDSMKPGSV IVDLSAEAGG
NCGYTKPGEV VKTPNRVTVV GYTDMPSRMA GQSSTLYANN ISKLLLSAGP FTGGPKGHFR
IDHADPVIRG SLVLEDGELR WPPPPGVIPQ ANTKVNETKD HKKDTGPVDL YEPTKRSALV
TTGAVAAALA LGATSPSAAF SSMLTKFGLA SICGYQTVWG VVPALHSPLM SVTNAVSGLT
AVGGMVLAGG GLLPGNTSQA LAGLAVAASA INIGGGFTIT QRMLDMFKRP TDPVEYNQLY
ALPAATLVGG YVAASLFMGG TGGDPEGVTS ASYLASSAMC IAAIACLANQ SSARTGNALG
MAGVGTGIAT TLGVMNAPAA VYAQVLTMLG AGIATGHYVG KTMKITELPQ MVAAFHSLVG
LAAVVTSIAN VMAADPGHLD MTHKVIAYLG DFIGAVTMTG SAIAFGKLHG VLKSDPLSLP
GKNAINLGLF AANLACGAAF LASDGSTDAG VLALVAAGVL AGVQGAHMTA SIGGADMPVV
ITLLNSYSGY ALCAEGFLLN NDLLTAVGAL IGSSGAILSY IMCTAMNRSL PNVILGGYGM
TAKAPADAAA VAGTHQEIDV GGVAEALTTA SKVIIVPGYG MAVANAQYPV AELAKHLTDK
GISVKFGIHP VAGRMPGQLN VLLAEAGVPY DVVFEMDEIN EEIEEADVCL VIGANDTVNS
AAVEDPNSVI AGMPVIEVWR SKQVFFMKRT MGAGYAGAEN PVFYKPNTFM FLGDAKKQTD
ALLARVNETL GIKA
//
