ID A8JNL4_DROME Unreviewed; 2081 AA.
AC A8JNL4;
DT 04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 2.
DT 27-MAR-2024, entry version 129.
DE SubName: Full=Still life, isoform N {ECO:0000313|EMBL:ABW08473.2};
GN Name=sif {ECO:0000313|EMBL:ABW08473.2,
GN ECO:0000313|FlyBase:FBgn0085447};
GN Synonyms=CG32414 {ECO:0000313|EMBL:ABW08473.2}, CG5256
GN {ECO:0000313|EMBL:ABW08473.2}, CG5406 {ECO:0000313|EMBL:ABW08473.2},
GN Dm_3L:26493 {ECO:0000313|EMBL:ABW08473.2}, Dmel\CG34418
GN {ECO:0000313|EMBL:ABW08473.2}, lincRNA.447
GN {ECO:0000313|EMBL:ABW08473.2}, RhoGEF {ECO:0000313|EMBL:ABW08473.2},
GN SIF {ECO:0000313|EMBL:ABW08473.2}, Sif {ECO:0000313|EMBL:ABW08473.2},
GN slf {ECO:0000313|EMBL:ABW08473.2}, Stilllife
GN {ECO:0000313|EMBL:ABW08473.2};
GN ORFNames=CG34418 {ECO:0000313|EMBL:ABW08473.2,
GN ECO:0000313|FlyBase:FBgn0085447}, Dmel_CG34418
GN {ECO:0000313|EMBL:ABW08473.2};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [3] {ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [5] {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [6] {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [7] {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [8] {ECO:0000313|EMBL:ABW08473.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
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DR EMBL; AE014296; ABW08473.2; -; Genomic_DNA.
DR RefSeq; NP_001097518.2; NM_001104048.2.
DR SMR; A8JNL4; -.
DR DNASU; 43892; -.
DR EnsemblMetazoa; FBtr0340654; FBpp0309505; FBgn0085447.
DR GeneID; 43892; -.
DR UCSC; CG34418-RG; d. melanogaster.
DR AGR; FB:FBgn0085447; -.
DR CTD; 43892; -.
DR FlyBase; FBgn0085447; sif.
DR VEuPathDB; VectorBase:FBgn0085447; -.
DR GeneTree; ENSGT00940000174094; -.
DR HOGENOM; CLU_000398_0_0_1; -.
DR BioGRID-ORCS; 43892; 0 hits in 3 CRISPR screens.
DR ChiTaRS; sif; fly.
DR GenomeRNAi; 43892; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0085447; Expressed in brain and 10 other cell types or tissues.
DR ExpressionAtlas; A8JNL4; baseline.
DR Genevisible; A8JNL4; DM.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0016043; P:cellular component organization; IEA:UniProt.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01230; PH1_Tiam1_2; 1.
DR CDD; cd01255; PH2_Tiam1_2; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 6.10.140.680; -; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 3.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR003116; RBD_dom.
DR InterPro; IPR043537; Tiam1/Tiam2/Sif.
DR InterPro; IPR040655; TIAM1_CC-Ex.
DR InterPro; IPR000697; WH1/EVH1_dom.
DR PANTHER; PTHR46001:SF2; PROTEIN STILL LIFE, ISOFORM SIF TYPE 1; 1.
DR PANTHER; PTHR46001; TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF02196; RBD; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF18385; Tiam_CC_Ex; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00233; PH; 2.
DR SMART; SM00455; RBD; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00461; WH1; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF50729; PH domain-like; 3.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50898; RBD; 1.
DR PROSITE; PS50229; WH1; 1.
PE 1: Evidence at protein level;
KW Proteomics identification {ECO:0007829|PeptideAtlas:A8JNL4};
KW Reference proteome {ECO:0000313|Proteomes:UP000000803}.
FT DOMAIN 29..147
FT /note="WH1"
FT /evidence="ECO:0000259|PROSITE:PS50229"
FT DOMAIN 842..955
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1121..1188
FT /note="RBD"
FT /evidence="ECO:0000259|PROSITE:PS50898"
FT DOMAIN 1204..1280
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 1445..1639
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT REGION 153..188
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 502..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 699..747
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1088..1119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1411..1434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1812..1841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1853..2048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2060..2081
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..477
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 508..553
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 561..575
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 631..655
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 699..722
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 730..747
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1097..1112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1922..1951
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1960..2000
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2001..2044
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2081 AA; 232107 MW; 9A2CB6046834E04F CRC64;
MGNKLSCSCA PLMRKAYRYE DSPWQSSRRR DGHLLSSFRL WAEVFHVSAS GAGTVKWQQV
SEDLVPVNIT CIQDSPECIF HITAYNSQVD KILDVRLVQP GTRIGQASEC FVYWKDPMTN
DTWGLNFTSP IDAKQFRECC SPSFKFSRKA SSSYSLKLDP PGKGKVKAKR KPLSTPASPS
RVRQEPQCTC MSAEQYARLR TDPRVRGSST LPRNVGSHRI TDVDGQQQVG SGKVVSAVSS
TSLYDNVASG GPGTNQGADT LPRQMKGGQQ DRQDVANSGV NTNTPGVIVT GVGNVGSDMC
GQNHVGSQVG NDDPAACQMD MDLSKSEGTQ AGGGLHQSVG TCTSSSKGTG TRNKDFGDDM
TRDAHSHDMH QHNVINNNTR RKTKSTEDMN VDTSTLKRML KPMPSTESPV TSPEMGRRRY
NYYNANAAQT LGHPPHMHQH GMAMGMGGGG GGGHHIMNNN TMGRASSQSS RFSGSRSSHE
IGRGYPPRNL YLELERERSC IEGSPPSDNV MFDNQCYATT PSSSNGNSDQ DQSYGQQQSS
GQHPQQQQGP PQRSSRHQHH HQQAPNVTPT PGSPTSRLLL EYEMHLRNTL AKGMDAESYS
LHTFEALLSQ SMENLANAKS STLPLPPHRP LSTIRDKERD RDRDGYYSDR NELIRERERE
RDRGYLSDHN SSFSNSRCAS CIGESARAQW FRHSDGWRSG SSTIGSGSGH GMMTQQIPGS
GHKRSPWDSL PSLRQDSSLN DSGYKSARAD SLEQRAEFIR QDSLRSEYLS DRESRYGIVQ
QASIESTDSR MCYLTSSEIS DDDRMSLTTA VSDEDDGESV MASPYKAKAT GTAASSFNCT
GAVRKAGFLS VKKWLLRKKH QIELARKRGW KGYWVCLKGT TLLFYPCDSR EGRSVEAAPK
HLIIVDGAIM QPIPEHPKRD YIFCLSTAFG DAYLFQAPCQ VELENWVNSI HSACAAAFAR
HRGKTGTLHL LQEEIFRLEK AIESDHKLKH MAELQQSVVT DQETRHQIQT QILQWEENLE
RLHCEQFRLR CYMASLQSGE LPNPKSLLTH VSRPTKNTLN KLGVFTVSSF HAFICARSPS
LLNNLLAGRG ATKRRPPMLS RSNSGSSRRS MQMNSRDEPE KTFKVAMPDN AYSTVYLRDA
MSVEEFLASA CARRNLNPME HFVRVKKRRD MEDHNYFVPH RNDLIENYLH NHEFVEVCMK
ILYQVELQRT TLEQMWGFSV EAELIENAER QDELCCYVSR VEDKSVAMHN GIIKGDEIMV
INGAIVSDLD MMYLESVLQE EQSLSMMMRS SRTEPPDLVG IMRVTDDMID SLVCPPPPTD
PPVMSEEMIT GLIVPAPGWN GTSKDLYSPE AESSPATSFV DPAAMAAQLA VGGVGGVVVG
GLGVAKPTSR TSSFEIENLL KTAEQVTGFC RSPQETRKSS PTGSVTSSVS TTALTPSRQL
TDAEKLRKVV MELVDTERTY VKHLNNLLEH YLEPMKRETF LSNAEINALF GNIHEIVTFQ
RQFLQNLEES LDLEPDFNKF EHCGQFRNVL FAIGSAFLYY VNHFKLYSSF CASHSKAQKV
LHPNEGNHAL QEFLAARNPK QQHSSTLESY LIKPIQRILK YPLLLQQMRN LTDTRADEHV
HLCEALKGME KVAEHINEMQ RIHEEYGAIF DHLFRQHQKS CKQPIDLSPG DLLYYGGVEW
LNISDFLGKI KKGLELHAMC FVFKSAVVFL CKERLRQKKK LMGVSSKNAT NEVEIIRYQV
LIPVTEVQVR ASSAKDMDSH FLWELIHLRS QLQRRSEKVY VLSNSTADFR NAFLKTIRQI
IRESVRNMSI PMKNFGGSSG SVSGHSSQGM GSMGYPGNSQ TLERPKQQIT IVHGSHTLGK
PKKKSGSQRH SAGNIDYDNL SGSQEADDLP PSVGVVHYAS GHTHGQQMQP AGFRGRSKTV
GDVTEITCSS PEPHQQQQQQ QQQQQQLMQQ GHAHAHPHPH PHPREPPPPP IRQPHLHHHS
SDIERIDPGT KSEGEEDSQQ GTIRPKATLG RTPNHLTLST TSTLSVGSTG SQARLIQSSH
PPASYQPVLM KDLGSPVWKP RDMINLGTDP QSTTRKDDVK N
//
