ID A8PQD4_9COXI Unreviewed; 757 AA.
AC A8PQD4;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN Name=ptsP {ECO:0000313|EMBL:EDP47038.1};
GN ORFNames=RICGR_1488 {ECO:0000313|EMBL:EDP47038.1};
OS Rickettsiella grylli.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC Rickettsiella.
OX NCBI_TaxID=59196 {ECO:0000313|EMBL:EDP47038.1, ECO:0000313|Proteomes:UP000054075};
RN [1] {ECO:0000313|EMBL:EDP47038.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Seshadri R., Federici B.A.;
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDP47038.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Myers G.S.;
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDP47038.1}.
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DR EMBL; AAQJ02000001; EDP47038.1; -; Genomic_DNA.
DR RefSeq; WP_006036000.1; NZ_AAQJ02000001.1.
DR AlphaFoldDB; A8PQD4; -.
DR STRING; 59196.RICGR_1488; -.
DR eggNOG; COG3605; Bacteria.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000054075; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244:SF1; PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:EDP47038.1};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EDP47038.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 17..163
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
SQ SEQUENCE 757 AA; 84396 MW; 8AC1698DC4696609 CRC64;
MLKMLRRIVQ EISSVQGFEK ALRILVRRIR EALNIQSCTV FILDDEKNYV LLATEGLNPH
CIRKIRFNFN QGLVGLVGQT RELINIENAP EHPDFLYITD VGEERYKAFL GVPIIHNRAL
LGVLVAQQEE QRCFDETEEA FLVTMAAQLG GVLAHAEATG EILSLFKENK KKLQQDIFFQ
GIASAPGIAI GEAVVVFPFA DLEAVPCKKV DNLSAEIIQL KKAFKAARDD IHRLKKHMMV
VLPEEEQALF DVYLAILNKA SLEKEVITTI KTTHQWAQAA LQLVINKHVQ QFEKVDDDYL
RERASDLRDL GRRILMHLQN NPFRPPRYPE NTILIGEEVS PSALAEVPEG RLIGIVSVKG
SSHSHFSILA RSLGIPAVTG VENLPVNQLE AQTLIVDGSQ GRIIIAPSER VKKSFTTLMR
QQSEITNSLE ALRELPAQTL DGYNIALWVN TGLMADASLS LTAGAEGIGL YRTEVPFLIR
DCFPAEDEQY GLYRQLLAAF SPRPVVMRTL DIGGDKALPY FPIKEDNPFL GWRGIRVTLD
HPEIFLIQIR AMLRANLEFN NLRIMLPMVS CIAEADHALR LIDQAYEKVV SEDSQACKPP
IGIMIEVPSA IYQVRGLAER VEFLSVGSND LTQYMLAVDR NNPRVANLYD PFQPAVLKAL
VHIVEAAHLA KKPISICGEM ASDPLAVPLL LAMGFDALSM NSFSIPRIKS VIRQTSLSQA
KNTLIHILAM DNAADIRKYL SEAVPQFNVD CLMPVTG
//
