ID   A8TLV2_9PROT            Unreviewed;      1013 AA.
AC   A8TLV2;
DT   15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT   15-JAN-2008, sequence version 1.
DT   13-SEP-2023, entry version 66.
DE   SubName: Full=Sarcosine oxidase alpha subunit {ECO:0000313|EMBL:EDP66180.1};
GN   ORFNames=BAL199_24574 {ECO:0000313|EMBL:EDP66180.1};
OS   alpha proteobacterium BAL199.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria.
OX   NCBI_TaxID=331869 {ECO:0000313|EMBL:EDP66180.1, ECO:0000313|Proteomes:UP000003417};
RN   [1] {ECO:0000313|EMBL:EDP66180.1, ECO:0000313|Proteomes:UP000003417}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BAL199 {ECO:0000313|EMBL:EDP66180.1,
RC   ECO:0000313|Proteomes:UP000003417};
RA   Hagstrom A., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G.,
RA   Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the GcvT family.
CC       {ECO:0000256|ARBA:ARBA00008609}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EDP66180.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; ABHC01000002; EDP66180.1; -; Genomic_DNA.
DR   AlphaFoldDB; A8TLV2; -.
DR   STRING; 331869.BAL199_24574; -.
DR   eggNOG; COG0404; Bacteria.
DR   eggNOG; COG0446; Bacteria.
DR   OrthoDB; 5287468at2; -.
DR   Proteomes; UP000003417; Unassembled WGS sequence.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.20.440; 2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain; 1.
DR   Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR   InterPro; IPR042204; 2Fe-2S-bd_N.
DR   InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR006277; Sarcosine_oxidase_asu.
DR   InterPro; IPR041117; SoxA_A3.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   NCBIfam; TIGR01372; soxA; 1.
DR   PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR   PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR   Pfam; PF12831; FAD_oxidored; 1.
DR   Pfam; PF13510; Fer2_4; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   Pfam; PF17806; SO_alpha_A3; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00469; PNDRDTASEII.
DR   SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF103025; Folate-binding domain; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000003417}.
FT   DOMAIN          524..606
FT                   /note="SoxA A3"
FT                   /evidence="ECO:0000259|Pfam:PF17806"
FT   DOMAIN          621..892
FT                   /note="Aminomethyltransferase folate-binding"
FT                   /evidence="ECO:0000259|Pfam:PF01571"
FT   DOMAIN          918..1005
FT                   /note="Glycine cleavage T-protein C-terminal barrel"
FT                   /evidence="ECO:0000259|Pfam:PF08669"
SQ   SEQUENCE   1013 AA;  108662 MW;  4802899CCF957D9E CRC64;
     MIFGRLPLEQ SHRLLTGGAI DRLKPLSFSF DGRSYRGYAG DTLASALLAN GVTVMGRSFK
     YHRPRGVMSA GPEEPNALVE LRSGARREPN TRATMIELFH GLRATSQNRW PCLRFDAMEV
     NDLLSRFLVA GFYYKTFMGF PGWHFWEKRI RAAAGMGAGT VEHDPDGYET MNAHCDVLVV
     GGGPAGLAAA LTAGRTGARV ILVDETDGLG GRLRSERDTL DGKPAIAWAA SVAAELADLP
     EVRVLTRTTA FGIYDNNLVG AVERLADHMP VPPAHVARQR LWHIRPQRVI VAAGAIERPL
     VFGGNDRPGV MLAGAVRTYL NRFAVTPGKR AVVVANNDDG YRTAFDLAAA GIDVMQILDT
     RSASHGAWRE RALAAGFDVA EGSAVTNAYG RMGLVGCDIA RLDGRGGTVG DSRFVPCDLI
     ANSGGWTPSI HLHSHAGGKA EWNDQIAAFL PGSVRSDVVT IGAARGRFAL SDCLSDGFKA
     GAEAASSCGF GTGTAPDAPI TDEVPTAPLQ ALWSVPPTPG KKVKRFVDHQ DDVTVEDIEL
     AHREGFVSVE HLKRYTTLGM GTDQGKTSNI NGHALMAAAR GQSIPTVGTT TFRPPYTPVA
     MGAFAGRTTG RHLAPTRRTA LHDWHAANGV VFIEAGPWLR PQYYLQPGEA VSKETMDRAI
     TDEVVTTRTK VGISDVSSLG KIDVQGPDSA EFLNRVYSNG FAALPVGKAR YGLMLREDGL
     VDDDGTTSRL SDTHFLMTTT TVHAAKVLAD LEWYLQVVWP ELDVRVSSVT EQWAGMALPG
     PKAREVLAAA VDPGADVSNE ALPYLGVTCT ATIRGVPVRI FRISFSGELG YEINAPADWG
     IAVWEAVMEA GEDHGIRPYG LEAMGIMRIE KGHVAGPELD GRTTPDDLGL GRMASTKKWF
     VGRGMLDKPA FTDPDRPKLV GLVPVDGVSR IRAGSILVDD PDAATPVPKL GHITSSAYLS
     PTVGHPISLG LLARGRERIG QEVWAVFPLR GIRVRARVTE TCFVDPEGKK LHG
//