ID A8XLQ0_CAEBR Unreviewed; 555 AA.
AC A8XLQ0;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 2.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 {ECO:0000256|ARBA:ARBA00021134};
DE EC=2.1.1.296 {ECO:0000256|ARBA:ARBA00012770};
GN ORFNames=CBG15196 {ECO:0000313|EMBL:CAP33554.2,
GN ECO:0000313|WormBase:CBG15196}, CBG_15196
GN {ECO:0000313|EMBL:CAP33554.2};
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238 {ECO:0000313|EMBL:CAP33554.2, ECO:0000313|Proteomes:UP000008549};
RN [1] {ECO:0000313|EMBL:CAP33554.2, ECO:0000313|Proteomes:UP000008549}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16 {ECO:0000313|EMBL:CAP33554.2,
RC ECO:0000313|Proteomes:UP000008549};
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-
CC ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine =
CC a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-
CC ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + H(+) + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:67024, Rhea:RHEA-COMP:17169,
CC Rhea:RHEA-COMP:17170, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:167612, ChEBI:CHEBI:167614;
CC EC=2.1.1.296; Evidence={ECO:0000256|ARBA:ARBA00024560};
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DR EMBL; HE601055; CAP33554.2; -; Genomic_DNA.
DR AlphaFoldDB; A8XLQ0; -.
DR STRING; 6238.A8XLQ0; -.
DR WormBase; CBG15196; CBP38227; WBGene00035518; -.
DR eggNOG; KOG3674; Eukaryota.
DR eggNOG; KOG3935; Eukaryota.
DR HOGENOM; CLU_432275_0_0_1; -.
DR InParanoid; A8XLQ0; -.
DR OMA; PYFENRS; -.
DR OrthoDB; 5488054at2759; -.
DR Proteomes; UP000008549; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004483; F:mRNA (nucleoside-2'-O-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IBA:GO_Central.
DR GO; GO:0097309; P:cap1 mRNA methylation; IBA:GO_Central.
DR GO; GO:0097310; P:cap2 mRNA methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.12760; -; 1.
DR InterPro; IPR025807; Adrift-typ_MeTrfase.
DR InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR16121:SF2; CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2; 1.
DR PANTHER; PTHR16121; UNCHARACTERIZED; 1.
DR Pfam; PF01728; FtsJ; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51614; SAM_MT_ADRIFT; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|PROSITE-ProRule:PRU00946};
KW Reference proteome {ECO:0000313|Proteomes:UP000008549};
KW S-adenosyl-L-methionine {ECO:0000256|PROSITE-ProRule:PRU00946};
KW Transferase {ECO:0000256|PROSITE-ProRule:PRU00946}.
FT DOMAIN 91..300
FT /note="Adrift-type SAM-dependent 2'-O-MTase"
FT /evidence="ECO:0000259|PROSITE:PS51614"
FT ACT_SITE 253
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 127
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 147
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
FT BINDING 213
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00946"
SQ SEQUENCE 555 AA; 64595 MW; 66CB73DE0B58B790 CRC64;
MSENTKLDAN PIEFMSNQEM DNYYNNHVFT FLDHQKMDSK LDTFEFQNHS KRLEEVALKI
RSINNSKSYD EHTDLMHDYW RCPNILRNHF ICKNKAFLKM LEILESGFLN HLPWDNLNSF
HLCEGPGYFL DAIYVHRLRT TSQHSEWHWG ANTLNPYFEN RSCFEKLIDD SHIRGHERNW
YFGPSDDGDV AKLTEDYLIE KGLKGTFDLV TADGSTNTQG KEGQIEEIVG PLIRAEVNAA
LLLLKPGGSL ILKTYRFCEK QIQDVMFLLA DNFSEIRVVK PMASRPGSSE RYLICNGFGG
HLPLPMDLLV LCDEFFIIRQ VSRMDLHVKT FENEHGNQKL SWKNRKRFME QMKSYVFEEK
IPKNLKKMFP SGTSPWLSLY GHDLIRRNTL ENQTFAIQNY INSANLFPKD NFDPEWIMDQ
ILGQSKMLVT SDIKLESKDS LFIEPIALQA LLSNQNLELT NLPTNGFWLS RISASMFVLL
KLVFQNMESG EGWLKWQGRI SNNRIHDLLS RLLLELSLLP DGTSIRCFVP IDVLDLFHQH
ICFQNVMILH SMKCL
//
