ID A9CIP6_AGRFC Unreviewed; 193 AA.
AC A9CIP6;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=Putative NAD(P)H nitroreductase {ECO:0000256|PIRNR:PIRNR000232};
DE EC=1.-.-.- {ECO:0000256|PIRNR:PIRNR000232};
GN OrderedLocusNames=Atu1744 {ECO:0000313|EMBL:AAK87513.2};
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299 {ECO:0000313|EMBL:AAK87513.2, ECO:0000313|Proteomes:UP000000813};
RN [1] {ECO:0000313|EMBL:AAK87513.2, ECO:0000313|Proteomes:UP000000813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970 {ECO:0000313|Proteomes:UP000000813};
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F.Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D.Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.Y., Dolan M., Chumley F., Tingey S.V., Tomb J.F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2] {ECO:0000313|EMBL:AAK87513.2, ECO:0000313|Proteomes:UP000000813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970 {ECO:0000313|Proteomes:UP000000813};
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3] {ECO:0007829|PDB:3K6H}
RP X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) IN COMPLEX WITH FMN.
RA Tan K., Xu X., Cui H., Savchenko A., Edwards A., Joachimiak A.;
RT "Crystal structure of a nitroreductase family protein from Agrobacterium
RT tumefaciens str. C58.";
RL Submitted (OCT-2009) to the PDB data bank.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|PIRSR:PIRSR000232-1};
CC Note=Binds 1 FMN per subunit. {ECO:0000256|PIRSR:PIRSR000232-1};
CC -!- SIMILARITY: Belongs to the nitroreductase family.
CC {ECO:0000256|ARBA:ARBA00007118, ECO:0000256|PIRNR:PIRNR000232}.
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DR EMBL; AE007869; AAK87513.2; -; Genomic_DNA.
DR RefSeq; NP_354728.2; NC_003062.2.
DR RefSeq; WP_010971840.1; NC_003062.2.
DR PDB; 3K6H; X-ray; 3.05 A; A/B=1-193.
DR PDBsum; 3K6H; -.
DR AlphaFoldDB; A9CIP6; -.
DR SMR; A9CIP6; -.
DR STRING; 176299.Atu1744; -.
DR EnsemblBacteria; AAK87513; AAK87513; Atu1744.
DR KEGG; atu:Atu1744; -.
DR PATRIC; fig|176299.10.peg.1756; -.
DR eggNOG; COG0778; Bacteria.
DR HOGENOM; CLU_070764_5_0_5; -.
DR OrthoDB; 9804207at2; -.
DR PhylomeDB; A9CIP6; -.
DR EvolutionaryTrace; A9CIP6; -.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-UniRule.
DR CDD; cd02135; YdjA-like; 1.
DR Gene3D; 3.40.109.10; NADH Oxidase; 1.
DR InterPro; IPR029479; Nitroreductase.
DR InterPro; IPR000415; Nitroreductase-like.
DR InterPro; IPR026021; YdjA-like.
DR PANTHER; PTHR43821; NAD(P)H NITROREDUCTASE YDJA-RELATED; 1.
DR PANTHER; PTHR43821:SF1; NAD(P)H NITROREDUCTASE YDJA-RELATED; 1.
DR Pfam; PF00881; Nitroreductase; 1.
DR PIRSF; PIRSF000232; YdjA; 1.
DR SUPFAM; SSF55469; FMN-dependent nitroreductase-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3K6H};
KW Flavoprotein {ECO:0000256|PIRNR:PIRNR000232, ECO:0007829|PDB:3K6H};
KW FMN {ECO:0000256|PIRNR:PIRNR000232, ECO:0000256|PIRSR:PIRSR000232-1};
KW NAD {ECO:0000256|PIRNR:PIRNR000232}; NADP {ECO:0000256|PIRNR:PIRNR000232};
KW Nucleotide-binding {ECO:0007829|PDB:3K6H};
KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR000232};
KW Reference proteome {ECO:0000313|Proteomes:UP000000813}.
FT DOMAIN 23..169
FT /note="Nitroreductase"
FT /evidence="ECO:0000259|Pfam:PF00881"
FT BINDING 14..16
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000256|PIRSR:PIRSR000232-1"
FT BINDING 14
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 15
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 16
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 41
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000256|PIRSR:PIRSR000232-1"
FT BINDING 44
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 45
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000256|PIRSR:PIRSR000232-1"
FT BINDING 138
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 139..141
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000256|PIRSR:PIRSR000232-1"
FT BINDING 141
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 178
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
FT BINDING 180
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0007829|PDB:3K6H"
SQ SEQUENCE 193 AA; 21486 MW; 4DAD8CE98EBB7073 CRC64;
MTSDIKLLDY LRVRRSTPAL QLSEPGPSKG EIEEILRLAV RVPDHGKLAP WRFVVYRGEE
RVRLSEAALR IALEKNPDLD LQQQEAERTR FTRAPVVIAV ISTAKPHFKI PEWEQVMSAG
AVCLNVIFAA NASGFAANWL TEWLAFDPAF LAEIGVSAEE KVAGYIHIGS TTFPPVERPR
PELADVVTWV GDV
//
