ID A9CJ11_AGRFC Unreviewed; 277 AA.
AC A9CJ11;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=S-formylglutathione hydrolase {ECO:0000256|ARBA:ARBA00012479, ECO:0000256|RuleBase:RU363068};
DE EC=3.1.2.12 {ECO:0000256|ARBA:ARBA00012479, ECO:0000256|RuleBase:RU363068};
GN OrderedLocusNames=Atu1476 {ECO:0000313|EMBL:AAK87266.1};
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299 {ECO:0000313|EMBL:AAK87266.1, ECO:0000313|Proteomes:UP000000813};
RN [1] {ECO:0000313|EMBL:AAK87266.1, ECO:0000313|Proteomes:UP000000813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970 {ECO:0000313|Proteomes:UP000000813};
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F.Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D.Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.Y., Dolan M., Chumley F., Tingey S.V., Tomb J.F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2] {ECO:0000313|EMBL:AAK87266.1, ECO:0000313|Proteomes:UP000000813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970 {ECO:0000313|Proteomes:UP000000813};
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3] {ECO:0007829|PDB:3E4D}
RP X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.
RX PubMed=19653299; DOI=10.1002/pro.216;
RA van Straaten K.E., Gonzalez C.F., Valladares R.B., Xu X., Savchenko A.V.,
RA Sanders D.A.;
RT "The structure of a putative S-formylglutathione hydrolase from
RT Agrobacterium tumefaciens.";
RL Protein Sci. 18:2196-2202(2009).
CC -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC formaldehyde. {ECO:0000256|RuleBase:RU363068}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + S-formylglutathione = formate + glutathione + H(+);
CC Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000080,
CC ECO:0000256|RuleBase:RU363068};
CC -!- SIMILARITY: Belongs to the esterase D family.
CC {ECO:0000256|ARBA:ARBA00005622, ECO:0000256|RuleBase:RU363068}.
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DR EMBL; AE007869; AAK87266.1; -; Genomic_DNA.
DR PIR; A97539; A97539.
DR PIR; AD2758; AD2758.
DR RefSeq; NP_354481.1; NC_003062.2.
DR RefSeq; WP_010971651.1; NC_003062.2.
DR PDB; 3E4D; X-ray; 2.01 A; A/B/C/D/E/F=1-277.
DR PDBsum; 3E4D; -.
DR AlphaFoldDB; A9CJ11; -.
DR SMR; A9CJ11; -.
DR STRING; 176299.Atu1476; -.
DR ESTHER; agrt5-a9cj11; A85-EsteraseD-FGH.
DR MEROPS; S09.940; -.
DR EnsemblBacteria; AAK87266; AAK87266; Atu1476.
DR GeneID; 66221685; -.
DR KEGG; atu:Atu1476; -.
DR PATRIC; fig|176299.10.peg.1500; -.
DR eggNOG; COG0627; Bacteria.
DR HOGENOM; CLU_056472_0_0_5; -.
DR OrthoDB; 9782200at2; -.
DR PhylomeDB; A9CJ11; -.
DR BioCyc; AGRO:ATU1476-MONOMER; -.
DR EvolutionaryTrace; A9CJ11; -.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000801; Esterase-like.
DR InterPro; IPR014186; S-formylglutathione_hydrol.
DR NCBIfam; TIGR02821; fghA_ester_D; 1.
DR PANTHER; PTHR10061; S-FORMYLGLUTATHIONE HYDROLASE; 1.
DR PANTHER; PTHR10061:SF0; S-FORMYLGLUTATHIONE HYDROLASE; 1.
DR Pfam; PF00756; Esterase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3E4D};
KW Hydrolase {ECO:0000256|RuleBase:RU363068};
KW Metal-binding {ECO:0007829|PDB:3E4D};
KW Reference proteome {ECO:0000313|Proteomes:UP000000813};
KW Serine esterase {ECO:0000256|RuleBase:RU363068}.
FT ACT_SITE 147
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT ACT_SITE 223
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT ACT_SITE 256
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT BINDING 90
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3E4D"
FT BINDING 91
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3E4D"
FT BINDING 94
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3E4D"
FT BINDING 97
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3E4D"
SQ SEQUENCE 277 AA; 31268 MW; F3010875E9C8FE9B CRC64;
MNIISQNTAF GGMQGVFSHQ SETLKSEMTF AVYVPPKAIH EPCPVVWYLS GLTCTHANVM
EKGEYRRMAS ELGLVVVCPD TSPRGNDVPD ELTNWQMGKG AGFYLDATEE PWSEHYQMYS
YVTEELPALI GQHFRADMSR QSIFGHSMGG HGAMTIALKN PERFKSCSAF APIVAPSSAD
WSEPALEKYL GADRAAWRRY DACSLVEDGA RFPEFLIDQG KADSFLEKGL RPWLFEEAIK
GTDIGLTLRM HDRYDHSYYF ISTFMDDHLK WHAERLG
//
