ID A9CS56_ENTBH Unreviewed; 347 AA.
AC A9CS56;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
GN ORFNames=EBI_21979 {ECO:0000313|EMBL:EDQ31215.1};
OS Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC Enterocytozoon.
OX NCBI_TaxID=481877 {ECO:0000313|EMBL:EDQ31215.1, ECO:0000313|Proteomes:UP000001742};
RN [1] {ECO:0000313|EMBL:EDQ31215.1, ECO:0000313|Proteomes:UP000001742}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H348 {ECO:0000313|EMBL:EDQ31215.1,
RC ECO:0000313|Proteomes:UP000001742};
RX PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA Keeling P.J.;
RT "Patterns of genome evolution among the microsporidian parasites
RT Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT bieneusi.";
RL PLoS ONE 2:E1277-E1277(2007).
RN [2] {ECO:0000313|EMBL:EDQ31215.1, ECO:0000313|Proteomes:UP000001742}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H348 {ECO:0000313|EMBL:EDQ31215.1,
RC ECO:0000313|Proteomes:UP000001742};
RX PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT Enterocytozoon bieneusi.";
RL PLoS Pathog. 5:E1000261-E1000261(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000256|RuleBase:RU003792};
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDQ31215.1}.
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DR EMBL; ABGB01000002; EDQ31215.1; -; Genomic_DNA.
DR RefSeq; XP_001828063.1; XM_001828011.1.
DR AlphaFoldDB; A9CS56; -.
DR STRING; 481877.A9CS56; -.
DR VEuPathDB; MicrosporidiaDB:EBI_21979; -.
DR HOGENOM; CLU_021971_4_0_1; -.
DR InParanoid; A9CS56; -.
DR OMA; CDARTYT; -.
DR OrthoDB; 12038at2759; -.
DR Proteomes; UP000001742; Unassembled WGS sequence.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProt.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142:SF27; PSEUDOU_SYNTH_1 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW Lyase {ECO:0000313|EMBL:EDQ31215.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001742};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW ECO:0000256|RuleBase:RU003792}.
FT DOMAIN 180..286
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
SQ SEQUENCE 347 AA; 41196 MW; 8E30ACB32E0EA8D2 CRC64;
MKRKIAFLWG YCGITYSGIQ FNNEINTLEK KLIDILCKLQ YISEKNAESA QKIKMKSASR
TDKGVSAIFN VTICKINQEL TENINQILKN EFSKAGFILY NIARVPKTFI PYRSARSREY
KYFIPIGVLN KNIAKSQIDI MQSSENYNIS IEKFKHDTFD WLNKSTSNTQ IIDKLNFILN
LFHGNHDFHN FTTQNVQGTT IRYIKHIYAN SIKKINGIEY VEVFLHGDSF IMHQIRKMIA
FAIFMCRNYD DNTIIEQYYN IIFSKNNYYI PKAPSEYLYL YNIHFDDFNK RTPYEKLSVD
YDTIVNFETF IQSCILTTQN LSQWIEFIIN MEFKWKNVFY LNNKKEF
//
