ID A9CXS2_HOEPD Unreviewed; 562 AA.
AC A9CXS2;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=HPDFL43_21002 {ECO:0000313|EMBL:EDQ35713.1};
OS Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Phyllobacteriaceae; Hoeflea.
OX NCBI_TaxID=411684 {ECO:0000313|EMBL:EDQ35713.1, ECO:0000313|Proteomes:UP000004291};
RN [1] {ECO:0000313|EMBL:EDQ35713.1, ECO:0000313|Proteomes:UP000004291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DFL-43 {ECO:0000313|EMBL:EDQ35713.1,
RC ECO:0000313|Proteomes:UP000004291};
RA Wagner-Dobler I., Ferriera S., Johnson J., Kravitz S., Beeson K.,
RA Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDQ35713.1, ECO:0000313|Proteomes:UP000004291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DFL-43 {ECO:0000313|EMBL:EDQ35713.1,
RC ECO:0000313|Proteomes:UP000004291};
RA Fiebig A.;
RL Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDQ35713.1}.
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DR EMBL; ABIA03000001; EDQ35713.1; -; Genomic_DNA.
DR AlphaFoldDB; A9CXS2; -.
DR STRING; 411684.HPDFL43_21002; -.
DR eggNOG; COG0389; Bacteria.
DR HOGENOM; CLU_028184_1_1_5; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000004291; Chromosome.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000004291};
KW Transferase {ECO:0000313|EMBL:EDQ35713.1}.
FT DOMAIN 48..186
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 273..381
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
SQ SEQUENCE 562 AA; 61912 MW; ED90CBE7DE7F61A1 CRC64;
MPQTIPTSKT HRQRFLSIAF THLPTDRIQR LRWGKSWITD RISDAHPETQ PLVVTGTVKN
AIRILALDSR ARAHGIRKGQ SLSDARALVH DLDCHQADAD ADHAALLRIA SWCERYTPLV
GLGANPDAER ETGVGGDHGL FMDITGCAHL MGGEAALVAD LEARLLAQGF HARLCLADTP
GAAWAMARHG AARIISEDSH GEAILPLSLS GLRLQPALVA SLGRVGLKTI GCITSLPRAP
LAARFGAGLM QRLDQALGRE TEAISPLMPV AELSTERRFA DPVTHHDAVT HVISALAAAM
IEPLERRGLG IRRCRLRLFR VDGHVAALEV QTARPLRDAR MIERLFAERL AGLHEDLDAG
FGFDLIRLDI LHADPFEAAQ GDMVAGGRAS EGHDALVDRL GARLGLDRVQ RFVMADTHIP
ERSFARIQVA RLNGGPGNGS GRWSGAHATA LAMELLPDQV EPDPLITRPL ILFDRPELLS
TIAEVPDGPP LRFRWRGVAY EVARAQGPER IACEWWRDGR GARTRDYFRV EDRQGYRFWM
FRHGLYGRET NEPAWYMHGL FA
//
