ID A9D0J2_HOEPD Unreviewed; 657 AA.
AC A9D0J2;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 24-JAN-2024, entry version 64.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=HPDFL43_02380 {ECO:0000313|EMBL:EDQ35007.1};
OS Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Phyllobacteriaceae; Hoeflea.
OX NCBI_TaxID=411684 {ECO:0000313|EMBL:EDQ35007.1, ECO:0000313|Proteomes:UP000004291};
RN [1] {ECO:0000313|EMBL:EDQ35007.1, ECO:0000313|Proteomes:UP000004291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DFL-43 {ECO:0000313|EMBL:EDQ35007.1,
RC ECO:0000313|Proteomes:UP000004291};
RA Wagner-Dobler I., Ferriera S., Johnson J., Kravitz S., Beeson K.,
RA Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDQ35007.1, ECO:0000313|Proteomes:UP000004291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DFL-43 {ECO:0000313|EMBL:EDQ35007.1,
RC ECO:0000313|Proteomes:UP000004291};
RA Fiebig A.;
RL Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDQ35007.1}.
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DR EMBL; ABIA03000002; EDQ35007.1; -; Genomic_DNA.
DR RefSeq; WP_007196268.1; NZ_CM002917.1.
DR AlphaFoldDB; A9D0J2; -.
DR STRING; 411684.HPDFL43_02380; -.
DR eggNOG; COG1874; Bacteria.
DR HOGENOM; CLU_012430_1_0_5; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000004291; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:EDQ35007.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:EDQ35007.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000004291}.
FT DOMAIN 8..392
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 405..595
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 144
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 316
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 105
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 324
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 657 AA; 73880 MW; F1D395799FCF8EB4 CRC64;
MNRTLGVCYY PEHWPEAQWV EDAARMASLG IVHVRIGEFA WSRLEPRRGV YEFDWLGRAI
DVLHQAGLKV VLGTPTATPP KWLVDEMPDM LPVGADGLTR KFGSRRHYDF AHDGYLEECK
RITLALAKAF GAHPGIAAWQ TDNEYDCHNT TLSYSPAAKA GFQAWLQRKY QSPEALNRAW
GNVFWSMEIS EFSEVELPNL TVTEPNPTHV MDFRRFASDM VVRFNKAQAD IIRAHSPGRD
VIHNFMGRTL VFDHFDVGAD LDISSWDSYP LGFLEDRSDQ NDDWKRQFAH AGDPDFQAFH
HDLYRATSKG RWWVMEQQPG PVNWAPHNPA PRNGMVRLWS WEAFAHGAET VSYFRWRQAP
FAQEQMHAGL LRSDSVEAQG FHEAAQVSSE LGALDRELSD SAGKADVAIV FDYPSAWAWE
IQPQGREFDY FRLVFDFYRT MRRLGLSIDI VPASAPDLEG YGLVTIPGLF TWTPALIEAV
RAYQGHVLIG PRSGSKTADF AIPDSLPPAL PKDILDVRVT HVESLREDTP VYLAEGGSFQ
YWREFLEAGP ETENILTTGD GVPGLVAQGN RRYLGGWPDQ GLLYDIIMRL ASRGGLVTKE
LRDGLRLRRH GNHVFAFNYG ATAFDLSALG WTGKPLLGDL MIEPSGVAIT PLSRAGS
//
