ID A9KP66_LACP7 Unreviewed; 807 AA.
AC A9KP66;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 90.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Cphy_1352 {ECO:0000313|EMBL:ABX41728.1};
OS Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
OS (Clostridium phytofermentans).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=357809 {ECO:0000313|EMBL:ABX41728.1, ECO:0000313|Proteomes:UP000000370};
RN [1] {ECO:0000313|Proteomes:UP000000370}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700394 / DSM 18823 / ISDg
RC {ECO:0000313|Proteomes:UP000000370};
RA Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L.,
RA LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K.,
RA Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T.,
RA Bruce D., Detter J.C., Han C., Kuske C., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E.A., Richardson P.;
RT "Complete genome sequence of Clostridium phytofermentans ISDg.";
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP000885; ABX41728.1; -; Genomic_DNA.
DR RefSeq; WP_012199382.1; NC_010001.1.
DR AlphaFoldDB; A9KP66; -.
DR STRING; 357809.Cphy_1352; -.
DR KEGG; cpy:Cphy_1352; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000000370; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000000370}.
FT DOMAIN 5..292
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 293..570
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 807 AA; 91896 MW; C9446C95913DB05D CRC64;
MSIYDSLNPE QRRAVEHDRG PMLILAGAGS GKTRVLTHRI AYLIEERNVN PYQIMALTFT
NKAAKEMRER VDKIVGYGAE NIWVSTFHST CVRILRRFIE TLGYDRNFTI YDTDDQKTLI
REVLKFLQID TKQTKERVFL SAISSAKDEG ISPEEFELSA LGDYNKMKIS NVYKEYQRRL
KTNNALDFDD LIMKTVELFQ SNTEALNYYR NRFRYIMVDE YQDTNTIQFK LIRLLAHSLN
EYNEVEHNLC VVGDDDQSIY KFRGANIRNI LDFEKEFPDA VVIKLEQNYR STKNILNAAN
EVIHNNLGRK DKSLWTDNED GEQVSFCQYD TDFAEATQVV DSIKTLNDQD GISFNNFAIL
YRTNAQSRVF EEKMIQRSVP YKIIGSINFY QRKEIKDLLA YLKTIDNGLD GIAVKRIINV
PKRGIGLTTL DRVEEFSVKN NMSFYDALRH AENIPGLGRS TSKITSFVSF IETIKSKLSS
DTFTLKDLME EILEGTGYLK ELEEADDESA EDRIGNINEL INKIVTYEVE ATEPPTLSGF
LEEVALVADI DSLEEDSNHV VLMTLHSAKG LEFPYVYLCG MEDGIFPSYM SINAENPELE
IEEERRLCYV GITRAMKQIH LSAARQRMMR GETQFNRPSR FINEIPRYLL TIQSAQNKGY
LAKDSYGESP IRNASPNFST QSRGMFDSDN PFDKSSSYND TMFDKATPSM RGTTGINSMP
SFSGQAKTAR GFQPSVGGTL PTKNFGNSNL GTLAYGVGDT VKHIKFGQGV VTNLTKGGRD
YEVTVDFGEQ GIKKMLSSFA KLEKIED
//
