ID A9QPK0_METI4 Unreviewed; 562 AA.
AC A9QPK0;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 93.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=ftfL {ECO:0000313|EMBL:ABX56658.1};
GN Synonyms=fhs {ECO:0000256|HAMAP-Rule:MF_01543}, mIS1
GN {ECO:0000313|EMBL:ACD82431.1};
GN OrderedLocusNames=Minf_0373 {ECO:0000313|EMBL:ACD82431.1};
OS Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain
OS V4)).
OC Bacteria; Verrucomicrobiota; Methylacidiphilae; Methylacidiphilales;
OC Methylacidiphilaceae; Methylacidiphilum (ex Ratnadevi et al. 2023).
OX NCBI_TaxID=481448 {ECO:0000313|EMBL:ABX56658.1};
RN [1] {ECO:0000313|EMBL:ABX56658.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=V4 {ECO:0000313|EMBL:ABX56658.1};
RX PubMed=18004300; DOI=10.1038/nature06411;
RA Dunfield P.F., Yuryev A., Senin P., Smirnova A.V., Stott M.B., Hou S.,
RA Ly B., Saw J.H., Zhou Z., Ren Y., Wang J., Mountain B.W., Crowe M.A.,
RA Weatherby T.M., Bodelier P.L.E., Liesack W., Feng L., Wang L., Alam M.;
RT "Methane oxidation by an extremely acidophilic bacterium of the phylum
RT Verrucomicrobia.";
RL Nature 450:879-882(2007).
RN [2] {ECO:0000313|EMBL:ACD82431.1, ECO:0000313|Proteomes:UP000009149}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate V4 {ECO:0000313|Proteomes:UP000009149}, and V4
RC {ECO:0000313|EMBL:ACD82431.1};
RX PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V.,
RA Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.,
RA Feng L., Wang L., Alam M.;
RT "Complete genome sequence of the extremely acidophilic methanotroph isolate
RT V4, Methylacidiphilum infernorum, a representative of the bacterial phylum
RT Verrucomicrobia.";
RL Biol. Direct 3:26-26(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; EU223916; ABX56658.1; -; Genomic_DNA.
DR EMBL; CP000975; ACD82431.1; -; Genomic_DNA.
DR AlphaFoldDB; A9QPK0; -.
DR STRING; 481448.Minf_0373; -.
DR KEGG; min:Minf_0373; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_0; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000009149; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}.
FT BINDING 71..78
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 562 AA; 61082 MW; 8F55499A3F046708 CRC64;
MKEKNLSSDL KIAREKKPLG IEEVAKKLSI PHDALERYGA FKAKISWEYL KELFCRPRRG
KLVLVTATTP TPAGEGKTTT AIGLTDGLSR LGQKAALCLR EPSMGPVFGA KGAATGSGLA
QLIPREEINL HFTGDFAAVA AAHNLLAALI DNHLYHGNDL GIDPRKISWG RVVDINDRAL
RKILVGLESK KSFPRFSFFD IVAASEVMAI LCLAQSYKDL RQRIADICIG RGRDDKNIDA
EELGAAGAMS VLLVHAFKPN LVQTLENNPA FVHGGPFGNI AHGCSSVVSL NAALRLADWV
VTEAGFGSDL GGEKFVNIVC RQSGLKPDCA VIVTTVRALK FHGGMTLDCL NQRDLYSLEK
GFPNLLRHIQ IVEEVLGIPA VVALNRFSAD SGEEIDWLGK RLGSLGYPFA VCNHWAQGGE
GAVELARRVL ERSRQLNCKL NFTYADGDPL IKKIEKIALN VYKAGSIALH PHAQEEIRVI
ERQGLDQLPV CMAKTQYSFS VDPRLRGAPE GHELFVRELR VAAGAKYILV LCGEINTMPG
LPKVPASSSM DIDEEGKVIG TI
//
