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Database: UniProt
Entry: A9WT85
LinkDB: A9WT85
Original site: A9WT85 
ID   UVRB_RENSM              Reviewed;         693 AA.
AC   A9WT85;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   18-SEP-2019, entry version 80.
DE   RecName: Full=UvrABC system protein B {ECO:0000255|HAMAP-Rule:MF_00204};
DE            Short=Protein UvrB {ECO:0000255|HAMAP-Rule:MF_00204};
DE   AltName: Full=Excinuclease ABC subunit B {ECO:0000255|HAMAP-Rule:MF_00204};
GN   Name=uvrB {ECO:0000255|HAMAP-Rule:MF_00204};
GN   OrderedLocusNames=RSal33209_2293;
OS   Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484
OS   / NBRC 15589 / NCIMB 2235).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae;
OC   Renibacterium.
OX   NCBI_TaxID=288705;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235;
RX   PubMed=18723615; DOI=10.1128/jb.00721-08;
RA   Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S.,
RA   Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J.,
RA   Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.;
RT   "Genome sequence of the fish pathogen Renibacterium salmoninarum
RT   suggests reductive evolution away from an environmental Arthrobacter
RT   ancestor.";
RL   J. Bacteriol. 190:6970-6982(2008).
CC   -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC       processing of DNA lesions. A damage recognition complex composed
CC       of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC       binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC       DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC       binding by UvrB and probably causes local melting of the DNA
CC       helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC       strands. Then UvrB probes one DNA strand for the presence of a
CC       lesion. If a lesion is found the UvrA subunits dissociate and the
CC       UvrB-DNA preincision complex is formed. This complex is
CC       subsequently bound by UvrC and the second UvrB is released. If no
CC       lesion is found, the DNA wraps around the other UvrB subunit that
CC       will check the other stand for damage. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC       lesions. Interacts with UvrC in an incision complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- DOMAIN: The beta-hairpin motif is involved in DNA binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SIMILARITY: Belongs to the UvrB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
DR   EMBL; CP000910; ABY24024.1; -; Genomic_DNA.
DR   RefSeq; WP_012245689.1; NC_010168.1.
DR   SMR; A9WT85; -.
DR   STRING; 288705.RSal33209_2293; -.
DR   PRIDE; A9WT85; -.
DR   EnsemblBacteria; ABY24024; ABY24024; RSal33209_2293.
DR   KEGG; rsa:RSal33209_2293; -.
DR   eggNOG; ENOG4105CCW; Bacteria.
DR   eggNOG; COG0556; LUCA.
DR   HOGENOM; HOG000073580; -.
DR   KO; K03702; -.
DR   OMA; RYMHSEI; -.
DR   OrthoDB; 95696at2; -.
DR   BioCyc; RSAL288705:G1GAP-2042-MONOMER; -.
DR   Proteomes; UP000002007; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00204; UvrB; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   InterPro; IPR036876; UVR_dom_sf.
DR   InterPro; IPR004807; UvrB.
DR   InterPro; IPR041471; UvrB_inter.
DR   InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR   PANTHER; PTHR24029; PTHR24029; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF02151; UVR; 1.
DR   Pfam; PF12344; UvrB; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46600; SSF46600; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00631; uvrb; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW   DNA repair; Excision nuclease; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN         1    693       UvrABC system protein B.
FT                                /FTId=PRO_1000077912.
FT   DOMAIN       35    188       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      438    600       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      648    683       UVR. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   NP_BIND      48     55       ATP. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   MOTIF       101    124       Beta-hairpin.
SQ   SEQUENCE   693 AA;  78025 MW;  606C7F3149AC6D7E CRC64;
     MSLAQQINRV VAPFEVISEY QPAGDQPAAI ADLTERINNG EKDVVLLGAT GTGKSATTAW
     LIEQVQRPTL VMVQNKTLAA QLANEFRELL PNNAVEYFVS YYDYYQPEAY VPQTDTFIEK
     DSSVNEEVER LRYSATNSLL TRRDVIVVAT VSCIYGLGTP EEYVAGMVTL KQGDRVDRDQ
     LLRQFVGIQY TRNDIDFHRG TFRVRGDTVE ITPMYEEQAL RIEFFGDEIE KIFTLHPLTG
     EIIREENEMY VFPASHYVAG PERMAKAITR IEDELAVRLK ELEGQNKLLE AQRLRMRTTY
     DLEMMQQMGF CNGIENYSRH IDGRDSGSAP SCLIDYFPDD FLLVIDESHV TLPQIGAMYE
     GDMSRKRTLV DHGFRLPSAM DNRPLKWDEF LERVGQTVYL SATPGKYEMA KADGVVQQII
     RPTGLIDPEV IIKPTKGQID DLLGEIRTRV ERDERVLVTT LTKRMAEDLT EYLLGHGVKV
     QYLHSDVDTL RRVELLRELR LGVFDVLVGI NLLREGLDLP EVSLVSILDA DKEGFLRSAT
     SLIQTIGRAA RNVSGEVHMY ADKITDSMAK AIDETNRRRA IQVAYNTEHG VDPTPLRKRI
     ADITDSLARE DADTKSLLES AGKGRSRGKA PVPVRHDGLA AVPAEDLVDL IEQLTAQMHS
     AAGELQFELA ARLRDEVGDL KKELRQMQSA GHA
//
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