ID A9Y8S8_STRLN Unreviewed; 382 AA.
AC A9Y8S8;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE SubName: Full=Aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme {ECO:0000313|EMBL:QMV04463.1};
DE SubName: Full=LmbS {ECO:0000313|EMBL:ABX00614.1};
GN ORFNames=GJU35_01475 {ECO:0000313|EMBL:QMV04463.1}, GJU35_43445
GN {ECO:0000313|EMBL:QMV11861.1}, SLCG_0244
GN {ECO:0000313|EMBL:AXG51399.1}, SLINC_0250
GN {ECO:0000313|EMBL:ANS62474.1};
OS Streptomyces lincolnensis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1915 {ECO:0000313|EMBL:ABX00614.1};
RN [1] {ECO:0000313|EMBL:ABX00614.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19085073; DOI=10.1007/s12223-008-0060-8;
RA Koberska M., Kopecky J., Olsovska J., Jelinkova M., Ulanova D., Man P.,
RA Flieger M., Janata J.;
RT "Sequence analysis and heterologous expression of the lincomycin
RT biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC
RT 25466.";
RL Folia Microbiol. (Praha) 53:395-401(2008).
RN [2] {ECO:0000313|EMBL:ANS62474.1, ECO:0000313|Proteomes:UP000092598}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 2936 {ECO:0000313|EMBL:ANS62474.1,
RC ECO:0000313|Proteomes:UP000092598};
RA Meng S.C.;
RT "Enhancement of antibiotic productionsby engineered nitrateutilization in
RT actinobacteria.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AXG51399.1, ECO:0000313|Proteomes:UP000253884}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LC-G {ECO:0000313|EMBL:AXG51399.1,
RC ECO:0000313|Proteomes:UP000253884};
RX PubMed=29574602; DOI=10.1007/s10295-018-2029-1;
RA Xu Y., Tan G., Ke M., Li J., Tang Y., Meng S., Niu J., Wang Y., Liu R.,
RA Wu H., Bai L., Zhang L., Zhang B.;
RT "Enhanced lincomycin production by co-overexpression of metK1 and metK2 in
RT Streptomyces lincolnensis.";
RL J. Ind. Microbiol. Biotechnol. 45:345-355(2018).
RN [4] {ECO:0000313|EMBL:QMV04463.1, ECO:0000313|Proteomes:UP000515335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B48 {ECO:0000313|EMBL:QMV04463.1,
RC ECO:0000313|Proteomes:UP000515335};
RX PubMed=32322696;
RA Wang R., Kong F., Wu H., Hou B., Kang Y., Cao Y., Duan S., Ye J., Zhang H.;
RT "Complete genome sequence of high-yield strain S. lincolnensis B48 and
RT identification of crucial mutations contributing to lincomycin
RT overproduction.";
RL Synth Syst Biotechnol 5:37-48(2020).
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933};
CC -!- SIMILARITY: Belongs to the DegT/DnrJ/EryC1 family.
CC {ECO:0000256|RuleBase:RU004508}.
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DR EMBL; EU124663; ABX00614.1; -; Genomic_DNA.
DR EMBL; CP016438; ANS62474.1; -; Genomic_DNA.
DR EMBL; CP022744; AXG51399.1; -; Genomic_DNA.
DR EMBL; CP046024; QMV04463.1; -; Genomic_DNA.
DR EMBL; CP046024; QMV11861.1; -; Genomic_DNA.
DR RefSeq; WP_067425667.1; NZ_CP046024.1.
DR AlphaFoldDB; A9Y8S8; -.
DR STRING; 1915.SLINC_0250; -.
DR KEGG; sls:SLINC_0250; -.
DR OrthoDB; 5342089at2; -.
DR Proteomes; UP000092598; Chromosome.
DR Proteomes; UP000253884; Chromosome.
DR Proteomes; UP000515335; Chromosome.
DR GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00616; AHBA_syn; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR InterPro; IPR000653; DegT/StrS_aminotransferase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR PANTHER; PTHR30244:SF34; DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE; 1.
DR PANTHER; PTHR30244; TRANSAMINASE; 1.
DR Pfam; PF01041; DegT_DnrJ_EryC1; 1.
DR PIRSF; PIRSF000390; PLP_StrS; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE 3: Inferred from homology;
KW Aminotransferase {ECO:0000313|EMBL:QMV04463.1};
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR000390-2,
KW ECO:0000256|RuleBase:RU004508};
KW Reference proteome {ECO:0000313|Proteomes:UP000092598};
KW Transferase {ECO:0000313|EMBL:QMV04463.1}.
FT ACT_SITE 183
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000390-1"
FT MOD_RES 183
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR000390-2"
SQ SEQUENCE 382 AA; 40923 MW; D4F776430A1DDB9F CRC64;
MSDYIPFAAP CFDTAEEEAV LRVVRSGWVS TGAEAQSFEE EFAAYIGVAH AVALTSCTAA
LHVALKAYGI GPGDEVIVPT MTFVATATSV VHAGAAPVLA DVGPEHLTLD PDQVKSLITE
RTKAVVPVHF GGRMAAMEPL RELCDSHGLT LLEDAAHTLP ARDGDAVAGR AGDASAFSFF
ATKPITTAEG GMLCTDDARV ADEARRWSLH GLSRGAVNRY RPGHSAAYDV DRPGHKYNMS
DLAAALGRAQ LAKAGRLHAR RTAIAEVYLR ELAGLDRLEL PAADTATNRS SWYLFPVRVH
GHRRDAFRQR LHALGVGTSV HFEPLHRFTW LRDHVVRTGQ GFPVADAAAD TLVSLPVFPA
MDDAAVSRVV AAVRRAHDEA GR
//
