ID AB9A_ARATH Reviewed; 950 AA.
AC Q9FLT5; F4K3L7;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 24-JAN-2024, entry version 132.
DE RecName: Full=ABC transporter A family member 9;
DE Short=ABC transporter ABCA.9;
DE Short=AtABCA9;
DE AltName: Full=ABC2 homolog 11;
GN Name=ABCA9; Synonyms=ATH11; OrderedLocusNames=At5g61730; ORFNames=MAC9.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, BIOTECHNOLOGY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=23269834; DOI=10.1073/pnas.1214159110;
RA Kim S., Yamaoka Y., Ono H., Kim H., Shim D., Maeshima M., Martinoia E.,
RA Cahoon E.B., Nishida I., Lee Y.;
RT "AtABCA9 transporter supplies fatty acids for lipid synthesis to the
RT endoplasmic reticulum.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:773-778(2013).
CC -!- FUNCTION: Mediates the transport of acyl-CoAs and/or free fatty acids
CC to the endoplasmic reticulum. Has no effect on the selectivity of fatty
CC acid incorporation into triacylglycerol or further desaturation steps.
CC {ECO:0000269|PubMed:23269834}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:23269834}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:23269834}.
CC -!- TISSUE SPECIFICITY: Highly expressed in siliques. Detected in
CC seedlings, rosette leaves, stems and flowers.
CC {ECO:0000269|PubMed:23269834}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the middle and late stages of
CC seed development. {ECO:0000269|PubMed:23269834}.
CC -!- DISRUPTION PHENOTYPE: Reduced seed size with abnormal morphology and
CC reduced triacylglycerol content. Retarded growth on medium lacking
CC sucrose. {ECO:0000269|PubMed:23269834}.
CC -!- BIOTECHNOLOGY: Overexpression of ABCA9 increases seed oil content.
CC {ECO:0000269|PubMed:23269834}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCA family.
CC CPR flippase (TC 3.A.1.211) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AED97510.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010069; BAB10073.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97510.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; ANM69292.1; -; Genomic_DNA.
DR RefSeq; NP_001330984.1; NM_001345493.1.
DR RefSeq; NP_200981.1; NM_125567.2.
DR AlphaFoldDB; Q9FLT5; -.
DR SMR; Q9FLT5; -.
DR STRING; 3702.Q9FLT5; -.
DR TCDB; 3.A.1.211.20; the atp-binding cassette (abc) superfamily.
DR iPTMnet; Q9FLT5; -.
DR PaxDb; 3702-AT5G61730-1; -.
DR ProteomicsDB; 244532; -.
DR EnsemblPlants; AT5G61730.2; AT5G61730.2; AT5G61730.
DR GeneID; 836295; -.
DR Gramene; AT5G61730.2; AT5G61730.2; AT5G61730.
DR KEGG; ath:AT5G61730; -.
DR Araport; AT5G61730; -.
DR TAIR; AT5G61730; ABCA9.
DR eggNOG; KOG0059; Eukaryota.
DR HOGENOM; CLU_000604_19_5_1; -.
DR InParanoid; Q9FLT5; -.
DR OrthoDB; 6951at2759; -.
DR PRO; PR:Q9FLT5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLT5; baseline and differential.
DR Genevisible; Q9FLT5; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015245; F:fatty acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005319; F:lipid transporter activity; IBA:GO_Central.
DR GO; GO:0006869; P:lipid transport; IBA:GO_Central.
DR GO; GO:0048316; P:seed development; IMP:UniProtKB.
DR CDD; cd03263; ABC_subfamily_A; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013525; ABC2_TM.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR026082; ABCA.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR19229:SF219; ABC TRANSPORTER A FAMILY MEMBER 9; 1.
DR PANTHER; PTHR19229; ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA; 1.
DR Pfam; PF12698; ABC2_membrane_3; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Endoplasmic reticulum; Lipid transport; Membrane;
KW Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..950
FT /note="ABC transporter A family member 9"
FT /id="PRO_0000240330"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 520..765
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 566..573
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 950 AA; 105431 MW; BEC9BE912192DB5E CRC64;
MTLREGLPLF HQQFTALFKK NLLLSWRNKR ATCLHLFSSF FFILLIFSIE ESSKASDLTS
TRHKNVTDPK ALVSLPILPC EDKFFVRLPC FDFVWSGNQS RRVTDIVSAI MANNPGRPIP
TNKVQSFTKP EEVDAWFMSH PSQVTGALHF VEKNATVISY GIQTNSSSEK KRGRREDPTF
KFLVPLQIAA EREIARSLIG DPKFSWDFGF KEFARPAIGG EVIISAFYLM GPVFFLAFSM
FGFVLQLGSV VTEKELKLRE AMTTMGVYES AYWLSWLIWE GILTFVSSLF LVLFGMMFQF
EFFLKNSFVL VFLLFFLFQF NMIGLAFALS SIISKSSSAT TVGFLVFLVG FITQIVTTAG
FPYSSAYSIG SRVIWSLFPP NTFSAGLQLL LEATSSPGDS GISWSERAIC AGGESTCVIT
TNKIYIWLVG TFFFWFVLAL YFDNIIPNAS GVRKSIFYFL KPSYWTGKEG NKVEEGSICS
CIGSVPPVEH ITPEDEDVLE EEILVKQQAM DGRVDPNIAV QIHGLAKTYP GTTKLGCCKC
TKTSPFHAVK GLWMNIAKDQ LFCLLGPNGA GKTTTISCLT GINPVTGGDA KIYGNSIRSS
VGMSNIRKMI GVCPQFDILW DALSSEEHLH LFASIKGLPP SSIKSIAEKL LVDVKLTGSA
KIRAGSYSGG MKRRLSVAIA LIGDPKLVFL DEPTTGMDPI TRRHVWDIIQ ESKKGRAIIL
TTHSMEEADI LSDRIGIMAK GRLRCIGTSI RLKSRFGTGF VATVSFIENK KDGAPEPLKR
FFKERLKVEP TEENKAFMTF VIPHDKEQLL KGFFAELQDR ESEFGIADIQ LGLATLEEVF
LNIARRAELE SATVEGTMVT LELESGIAVE IPVGARFVGI PGTENAENPR GLMVEVYWQQ
DGSGSMCISG HSAEMRIPEN VSVIYEPSSQ VLGHGQRRVR GIVIDYESNN
//
